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Transfection of the Human Malaria Parasite Plasmodium falciparum
Abstract
Methods to transiently and stably transfect blood stages of the human malaria parasite Plasmodium falciparum have been developed and adapted for gene-knockout, allelic replacement, and transgene expression in this organism. These methods are detailed in this chapter, as are approaches used to monitor transfectants during the selection process. The different plasmid vectors that are currently used for gene targeting and transgene expression (including green fluorescent protein expression) are also described.
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Citations
Cited by
An FKBP destabilization domain modulates protein levels in Plasmodium falciparum
Armstrong, C. M., & Goldberg, D. E. (2007) · Nature Methods · doi:10.1038/nmeth1132 · Cited by 261
Cellular adhesive phenomena in apicomplexan parasites of red blood cells
Cooke, B. M., Mohandas, N., Cowman, A. F., & Coppel, R. L. (2005) · Veterinary Parasitology · doi:10.1016/j.vetpar.2005.07.002 · Cited by 51
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Metadata
- Authors
- Crabb, B. S., Rug, M., Gilberger, T.-W., Thompson, J. K., Triglia, T., Maier, A. G., & Cowman, A. F.
- Venue
- Parasite Genomics Protocols
- Publisher
- Humana Press
- Published
- 2004-05-20
- Volume
- 270
- Pages
- 263-276
- OpenAlex
- W2009106952
- Cited by
- 178
- References
- 0
- FWCI
- 59.27
- Type
- book chapter
- Language
- en
- Open access
- closed
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- Crabb, B. S., Rug, M., Gilberger, T.-W., Thompson, J. K., Triglia, T., Maier, A. G., & Cowman, A. F. (2004). Transfection of the Human Malaria Parasite Plasmodium falciparum. Parasite Genomics Protocols. https://doi.org/10.1385/1-59259-793-9%3A263
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