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Eradication of Mycoplasma Contaminations
Abstract
Mycoplasma contaminations have a multitude of effects on cultured cell lines that can potentially influence the results of experiments and pollute bioactive substances used in human medicine. The elimination of mycoplasma contaminations in cell cultures has become a practical alternative to discarding and re-establishing important or irreplaceable cell lines. Different quinolones, tetracyclins, and macrolides shown to have strong antimycoplasma properties are employed for the decontamination. We provide detailed descriptions to assure eradication of mycoplasma, to prevent formation of resistant mycoplasma strains, and to cure heavily contaminated and damaged cells. To date, no consistent and permanent alterations that affect the eukaryotic cells during or after the treatment have been detected.
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Of all existing methods, antibiotic therapy remains the most widespread and relatively effective for decontaminating cell cultures from mycoplasmas.
“Of all the existing methods, antibiotic therapy remains the most widespread and relatively effective [52].”
From Mycoplasma Contamination of Primary Cell Cultures (2026) · cites this paper as [52]
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Cited by 13
Metadata
- Authors
- Uphoff, C. C., & Drexler, H. G.
- Affiliations
- Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures (DE)
- Venue
- Basic Cell Culture Protocols
- Publisher
- Humana Press
- Published
- 2004-09-10
- Volume
- 290
- Pages
- 025-034
- OpenAlex
- W4229707959
- Cited by
- 13
- References
- 0
- Citations by year
- 2026: 1 · 2017: 1 · 2016: 1 · 2012: 4
- FWCI
- 7.00
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Humana Press eBooks (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
- Keywords
- mycoplasma contamination, quinolones, tetracycline, macrolides
- MeSH terms
- Anti-Bacterial Agents, Cell Line, Mycoplasma
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