The DOI matches its Crossref record.
The HaloTag™: A Novel Technology for Cell Imaging and Protein Analysis
Abstract
The ability to specifically label proteins with a wide range of optical properties and functionalities can help reveal information about protein functions and dynamics in living cells. Here, we describe a technology for covalent tethering of organic probes directly to a specially designed reporting protein expressed in live cells. The reporting protein can be used in a manner similar to green fluorescent protein, except that the fluorophore might be interchanged among a variety of standard dyes. This allows living cells to be imaged at different wavelengths without requiring changes to the underlying genetic constructs, and the colors can be rapidly switched to allow temporal analysis of protein fate. The stability of the bond permits imaging of live cells during long time periods, imaging of fixed cells, and multiplexing with different cell/protein analysis techniques. The dyes can also be exchanged with other functional molecules, such as biotin to serve as an affinity handle, or even solid supports for direct covalent immobilization. The technology complements existing methods and provides new options for cell imaging and protein analysis.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis
Los, G. V., Encell, L. P., McDougall, M. G., Hartzell, D. D., Karassina, N., Zimprich, C., Wood, M. G., Learish, R., Ohana, R. F., Urh, M., et al. (2008) · ACS Chemical Biology · doi:10.1021/cb800025k · Cited by 2,624
Self-citationInduced protein degradation: an emerging drug discovery paradigm
Lai, A. C., & Crews, C. M. (2017) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2016.211 · Cited by 1,486
Detection of immunogenic cell death and its relevance for cancer therapy
Fucikova, J., Kepp, O., Kasikova, L., Petroni, G., Yamazaki, T., Liu, P., Zhao, L., Spisek, R., Kroemer, G., & Galluzzi, L. (2020) · Cell Death & Disease · doi:10.1038/s41419-020-03221-2 · Cited by 1,138
Radiation fosters dose-dependent and chemotherapy-induced immunogenic cell death
Golden, E. B., Frances, D., Pellicciotta, I., Demaria, S., Helen Barcellos-Hoff, M., & Formenti, S. C. (2014) · OncoImmunology · doi:10.4161/onci.28518 · Cited by 624
Gassen, N. C., Niemeyer, D., Muth, D., Corman, V. M., Martinelli, S., Gassen, A., Hafner, K., Papies, J., Mösbauer, K., Zellner, A., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-13659-4 · Cited by 356
Metadata
- Authors
- Los, G. V., & Wood, K.
- Venue
- High Content Screening
- Publisher
- Humana Press
- Published
- 2006-09-20
- Volume
- 356
- Pages
- 195-208
- OpenAlex
- W73139964
- Cited by
- 228
- References
- 0
- FWCI
- 14.48
- Type
- book chapter
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: The HaloTag™: A Novel Technology for Cell Imaging and Protein Analysis. https://app.cochat.ai/papers/10.1385/1-59745-217-3:195
- The paper
- Los, G. V., & Wood, K. (2006). The HaloTag™: A Novel Technology for Cell Imaging and Protein Analysis. High Content Screening. https://doi.org/10.1385/1-59745-217-3%3A195
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
