Cited by 2,892Carcinogens and Genotoxicity AssessmentDNA Repair MechanismsEffects of Radiation Exposure

The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.

The Comet Assay for DNA Damage and Repair: Principles, Applications, and Limitations

Collins, Andrew R.·2004·Molecular Biotechnology

Open this paper in CoChat — free account

Abstract

The comet assay (single-cell gel electrophoresis) is a simple method for measuring deoxyribonucleic acid (DNA) strand breaks in eukaryotic cells. Cells embedded in agarose on a microscope slide are lysed with detergent and high salt to form nucleoids containing supercoiled loops of DNA linked to the nuclear matrix. Electrophoresis at high pH results in structures resembling comets, observed by fluorescence microscopy; the intensity of the comet tail relative to the head reflects the number of DNA breaks. The likely basis for this is that loops containing a break lose their supercoiling and become free to extend toward the anode. The assay has applications in testing novel chemicals for genotoxicity, monitoring environmental contamination with genotoxins, human biomonitoring and molecular epidemiology, and fundamental research in DNA damage and repair. The sensitivity and specificity of the assay are greatly enhanced if the nucleoids are incubated with bacterial repair endonucleases that recognize specific kinds of damage in the DNA and convert lesions to DNA breaks, increasing the amount of DNA in the comet tail. DNA repair can be monitored by incubating cells after treatment with damaging agent and measuring the damage remaining at intervals. Alternatively, the repair activity in a cell extract can be measured by incubating it with nucleoids containing specific damage.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1385/mb:26:3:249

Cites

46 of 46 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

A simple technique for quantitation of low levels of DNA damage in individual cells
Singh, N. P., McCoy, M. T., Tice, R. R., & Schneider, E. L. (1988) · Experimental Cell Research · doi:10.1016/0014-4827(88)90265-0 · Cited by 10,828
Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing
Tice, R. R., Agurell, E., Anderson, D. C., Burlinson, B., Hartmann, A., Kobayashi, H., Miyamae, Y., Rojas, E., Ryu, J., & Sasaki, Y. F. (2000) · Environmental and Molecular Mutagenesis · doi:10.1002/(sici)1098-2280(2000)35:3 · Cited by 4,885
Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells
Östling, O., & Johanson, K. J. (1984) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(84)90411-x · Cited by 1,689
Heterogeneity in Radiation-Induced DNA Damage and Repair in Tumor and Normal Cells Measured Using the "Comet" Assay
Olive, P. L., Banáth, J. P., Durand, R. E., & Banáth, J. P. (1990) · Radiation Research · doi:10.2307/3577587 · Cited by 1,095
Recommendations for conducting the in vivo alkaline Comet assay
Hartmann, A. (2002) · Mutagenesis · doi:10.1093/mutage/18.1.45 · Cited by 1,064
Heterogeneity in Radiation-Induced DNA Damage and Repair in Tumor and Normal Cells Measured Using the “Comet” Assay
Olive, P. L., Banáth, J. P., & Durand, R. E. (2012) · Radiation Research · doi:10.1667/rrav04.1 · Cited by 1,031
Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA
Collins, A. R., Duthie, S. J., & Dobson, V. L. (1993) · Carcinogenesis · doi:10.1093/carcin/14.9.1733 · Cited by 822
Self-citation
The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cells
Collins, A. R., Aiguo, M., & Duthie, S. J. (1995) · Mutation Research/DNA Repair · doi:10.1016/0921-8777(94)00043-6 · Cited by 701
Self-citation
The comet assay: what can it really tell us?
Collins, A. R., Dobson, V. L., Dušinská, M., Kennedy, G., & Štětina, R. (1997) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/s0027-5107(97)00013-4 · Cited by 667
Self-citation
Oxidative Damage to DNA: Do We Have a Reliable Biomarker?
Collins, A. R., Dušinská, M., Gedik, C. M., & Štětina, R. (1996) · Environmental Health Perspectives · doi:10.1289/ehp.96104s3465 · Cited by 657
Self-citation

Show all 46 references

Research works printed but not linked: 1

[36]36. Novotná, B. (2000) Increased DNA fragmentation detected by comet assay in peripheral blood of heterozygotes for a frameshift mutation in the DNA repair protein nibrin. DNA Repair Workshop Smolenice, Slovakia, October 2000, Abstract p. 33.

Sorted by citations

Items 901–1,000 of 1,004

In Vitro Safety/Protection Assessment of Resveratrol and Pterostilbene in a Human Hepatoma Cell Line (HepG2)
Lombardi, G., Vannini, S., Blasi, F., Marcotullio, M. C., Dominici, L., Villarini, M., Cossignani, L., & Moretti, M. (2015) · Natural Product Communications · doi:10.1177/1934578x1501000823 · Cited by 28
Are the damaging effects induced by the imazethapyr formulation Pivot® H in Boana pulchella (Anura) reversible upon ceasing exposure?
Iglesias, J. M. P., Natale, G. S., Soloneski, S., & Larramendy, M. L. (2017) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2017.10.009 · Cited by 28
Radiosensitizing effect of the fucoidan from brown alga Fucus evanescens and its derivative in human cancer cells
Malyarenko, O. S., Zdobnova, E. V., Silchenko, A. S., Игоревич, К. М., & Ermakova, S. P. (2018) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2018.10.083 · Cited by 29
Chemopreventive role of Copaifera reticulata Ducke oleoresin in colon carcinogenesis
Senedese, J. M., Neto, F. R., Furtado, R. A., Nicollela, H. D., de Souza, L. D. R., Ribeiro, A. B., Ferreira, L. S., Magalhães, G. M., Carlos, I. Z., da Silva, J. J. M., et al. (2018) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2018.12.091 · Cited by 28
Lipopolysaccharide‐induced DNA damage response activates nuclear factor κB signalling pathway via GATA4 in dental pulp cells
Qiao, W., Huang, Y., Bian, Z., Sun, X., Wang, X. W., Gao, Q., Peng, Y., & Meng, L. (2019) · International Endodontic Journal · doi:10.1111/iej.13180 · Cited by 27
Additive effects of a small molecular PCNA inhibitor PCNA-I1S and DNA damaging agents on growth inhibition and DNA damage in prostate and lung cancer cells
Lu, S., & Dong, Z. (2019) · PLoS ONE · doi:10.1371/journal.pone.0223894 · Cited by 27
Classification of DNA damages on segmented comet assay images using convolutional neural network
Atila, Ü., Baydi̇lli̇, Y. Y., Şehirli, E., & Turan, M. K. (2019) · Computer Methods and Programs in Biomedicine · doi:10.1016/j.cmpb.2019.105192 · Cited by 30
Application of Negative Design To Design a More Desirable Virtual Screening Library
Yang, Z., He, J.-H., Lü, A., Hou, T., & Cao, D. (2020) · Journal of Medicinal Chemistry · doi:10.1021/acs.jmedchem.9b01476 · Cited by 29
Genetic damage in coal and uranium miners
Júnior, F. M. R. D. S., Tavella, R. A., Fernandes, C. L. F., & dos Santos, M. (2021) · Mutation Research/Genetic Toxicology and Environmental Mutagenesis · doi:10.1016/j.mrgentox.2021.503348 · Cited by 28
The miniaturized enzyme-modified comet assay for genotoxicity testing of nanomaterials
Yamani, N. E., Rundén‐Pran, E., Collins, A. R., Longhin, E., Elje, E., Hoet, P., Vrček, I. V., Doak, S. H., Fessard, V., & Dušinská, M. (2022) · Frontiers in Toxicology · doi:10.3389/ftox.2022.986318 · Cited by 28
Self-citation
Associations of subjective vitality with DNA damage, cardiovascular risk factors and physical performance
Maynard, S., Keijzers, G., Hansen, Å. M., Osler, M., Molbo, D., Bendix, L., Möller, P., Loft, S. H., Moreno‐Villanueva, M., Bürkle, A., et al. (2014) · Acta Physiologica · doi:10.1111/apha.12296 · Cited by 29
Sensitivity and specificity of the empirical lymphocyte genome sensitivity (LGS) assay: implications for improving cancer diagnostics
Anderson, D. C., Najafzadeh, M., Gopalan, R. C., Ghaderi, N., Scally, A., Britland, S. T., Jacobs, B. K., Reynolds, P., Davies, J. D., Wright, A. L., et al. (2014) · The FASEB Journal · doi:10.1096/fj.14-254748 · Cited by 28
Blood mononucleocytes are sensitive to the DNA damaging effects of iron overload—In vitro and ex vivo results with human and rat cells
Park, E., Glei, M., Knöbel, Y., & Pool-Zobel, B. L. (2007) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/j.mrfmmm.2007.01.012 · Cited by 28
DNA Damaging Effects of the Dyes Used in Sentinel Node Biopsy: Possible Implications for Clinical Practice
Masannat, Y., Hanby, A. M., Horgan, K. J., & Hardie, L. J. (2008) · Journal of Surgical Research · doi:10.1016/j.jss.2008.07.039 · Cited by 28
Quantification of metal ion induced DNA damage with single cell array based assay
Qiao, Y., & Ma, L. (2013) · The Analyst · doi:10.1039/c3an00967j · Cited by 28
Structure–activity relationship and role of oxygen in the potential antitumour activity of fluoroquinolones in human epithelial cancer cells
Perucca, P., Savio, M., Cazzalini, O., Mocchi, R., Maccario, C., Sommatis, S., Ferraro, D., Pizzala, R., Pretali, L., Fasani, E., et al. (2014) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/j.jphotobiol.2014.07.006 · Cited by 28
Experiences with the in vivo and in vitro comet assay in regulatory testing
Frötschl, R. (2014) · Mutagenesis · doi:10.1093/mutage/geu069 · Cited by 28
Composite-induced toxicity in human gingival and pulp fibroblast cells
Tadin, A., Marović, D., Galić, N., Kovacic, I., & Želježić, D. (2013) · Acta Odontologica Scandinavica · doi:10.3109/00016357.2013.824607 · Cited by 28
A classification framework and practical guidance for establishing a mode of action for chemical carcinogens
Butterworth, B. E. (2006) · Regulatory Toxicology and Pharmacology · doi:10.1016/j.yrtph.2006.01.011 · Cited by 28
Usage of the standard and modified comet assay in assessment of DNA damage in human lymphocytes after exposure to ionizing radiation
Mikloš, M., Gajski, G., & Garaj‐Vrhovac, V. (2009) · Radiology and Oncology · doi:10.2478/v10019-009-0015-y · Cited by 28
Alleviation of Aflatoxin B1‐Induced Genomic Damage by Proanthocyanidins via Modulation of DNA Repair
Bakheet, S. A., Alhuraishi, A. M., Al-Harbi, N. O., Alhosaini, K. A., AlSharari, S. D., Attia, M. M., Alhoshani, A., Al‐Shabanah, O. A., Al‐Harbi, M. M., Imam, F., et al. (2016) · Journal of Biochemical and Molecular Toxicology · doi:10.1002/jbt.21823 · Cited by 28
Clinical and genomic safety of treatment with Ginkgo biloba L. leaf extract (IDN 5933/Ginkgoselect®Plus) in elderly: a randomised placebo-controlled clinical trial [GiBiEx]
Bonassi, S., Prinzi, G., Lamonaca, P., Russo, P., Paximadas, I., Rasoni, G., Rossi, R., Ruggi, M., Malandrino, S., Sánchez-Flores, M., et al. (2018) · BMC Complementary and Alternative Medicine · doi:10.1186/s12906-018-2080-5 · Cited by 28
Ecotoxicological evaluation of gilthead seabream (Sparus aurata) exposed to the antibiotic oxytetracycline using a multibiomarker approach
Rodrigues, S., Antunes, S. C., Correia, A. T., & Nunes, B. (2018) · Marine Environmental Research · doi:10.1016/j.marenvres.2018.09.009 · Cited by 27
Effect of organic and in-organic chromium supplementation on growth performance and genotoxicity of Labeo rohita
Asad, F., Mubarik, M. S., Ali, T., Zahoor, M. K., Ashrad, R., & Qamer, S. (2018) · Saudi Journal of Biological Sciences · doi:10.1016/j.sjbs.2018.12.015 · Cited by 28
Mercuric dichloride induces DNA damage in human salivary gland tissue cells and lymphocytes
Schmid, K., Sassen, A. W., Staudenmaier, R., Kroemer, S., Reichl, F., Harréus, U., Hagen, R., & Kleinsasser, N. H. (2007) · Archives of Toxicology · doi:10.1007/s00204-007-0208-3 · Cited by 28
Oxidative damage induced genotoxic effects in human fibroblasts from Xeroderma Pigmentosum group A patients
Low, G. K. M., Fok, E. D. Z., Ting, A. P. L., & Hande, M. P. (2008) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2008.05.009 · Cited by 27
3,5,7,3′,4′-Pentamethoxyflavone, a quercetin derivative protects DNA from oxidative challenges: Potential mechanism of action
Jakhar, R., Paul, S., Park, Y. R., Han, J., & Kang, S. C. (2014) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/j.jphotobiol.2014.01.003 · Cited by 27
Effect of Chokeberry (Aronia melanocarpa) Juice on the Metabolic Activation and Detoxication of Carcinogenic N-Nitrosodiethylamine in Rat Liver
Krajka‐Kuźniak, V., Szaefer, H., Ignatowicz, E., Adamska, T., Oszmiański, J., & Baer‐Dubowska, W. (2009) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf803973y · Cited by 27
Effects of Tapinanthus globiferus and Zanthoxylum zanthoxyloides extracts on human leukocytes in vitro
Ogunbolude, Y., Ibrahim, M., Elekofehinti, O. O., Adeniran, A., Abolaji, A. O., Rochab, J., & Kamdem, J. P. (2014) · Journal of Intercultural Ethnopharmacology · doi:10.5455/jice.20140826110059 · Cited by 27
A novel drug conjugate, NEO212, targeting proneural and mesenchymal subtypes of patient-derived glioma cancer stem cells
Jhaveri, N., Agasse, F., Armstrong, D. L., Peng, L., Commins, D., Wang, W., Rosenstein-Sisson, R., Vaikari, V. P., Santiago, S., Santos, T., et al. (2015) · Cancer Letters · doi:10.1016/j.canlet.2015.11.040 · Cited by 27
Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin
Kračun‐Kolarević, M., Kolarević, S., Jovanović, J., Marković, V., Ilić, M., Simonović, P. D., Simić, V., Gačić, Z. M., Diamantini, E., Stella, E., et al. (2016) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2016.07.099 · Cited by 27
Synthesis methods influence characteristics, behaviour and toxicity of bare CuO NPs compared to bulk CuO and ionic Cu after in vitro exposure of Ruditapes philippinarum hemocytes
Volland, M., Hampel, M., Katsumiti, A., Yeste, M. P., Gatica, J. M., Cajaraville, M. P., & Blasco, J. (2018) · Aquatic Toxicology · doi:10.1016/j.aquatox.2018.04.007 · Cited by 27
Cytogenotoxic effects of Adenium obesum seeds extracts on breast cancer cells
Ali, A. Q., Farah, M. A., Abou-Tarboush, F. M., Al‐Anazi, K. M., Ali, M. A., Lee, J., Hailan, W. A. Q., & Mahmoud, A. H. (2018) · Saudi Journal of Biological Sciences · doi:10.1016/j.sjbs.2018.12.014 · Cited by 27
Genetic and epigenetic alterations induced by the small-molecule panobinostat: A mechanistic study at the chromosome and gene levels
Al‐Hamamah, M. A., Alotaibi, M. R., Ahmad, S. F., Ansari, M. A., Attia, M. S. M., Nadeem, A., Bakheet, S. A., Sobeai, H. M. A., & Attia, S. M. (2019) · DNA repair · doi:10.1016/j.dnarep.2019.03.008 · Cited by 27
Jujube honey induces apoptosis in human hepatocellular carcinoma HepG2 cell via DNA damage, p53 expression, and caspase activation
Cheng, N., Zhao, H., Chen, S., He, Q., & Cao, W. (2019) · Journal of Food Biochemistry · doi:10.1111/jfbc.12998 · Cited by 27
Testicular protective effects of ellagic acid on monosodium glutamate-induced testicular structural alterations in male rats
Hamza, R. Z., & Albaqami, N. M. (2019) · Ultrastructural Pathology · doi:10.1080/01913123.2019.1671569 · Cited by 27
Cytotoxic, Genotoxic, and Apoptotic Effects of Nickel Oxide Nanoparticles in Intestinal Epithelial Cells
Abudayyak, M., Meydanlı, E. G., & Özhan, G. (2020) · Turkish Journal of Pharmaceutical Sciences · doi:10.4274/tjps.galenos.2019.76376 · Cited by 27
Ongoing repair of migration-coupled DNA damage allows planarian adult stem cells to reach wound sites
Sahu, S., Sridhar, D., Abnave, P., Kosaka, N., Dattani, A., Thompson, J. M., Hill, M. A., & Aboobaker, A. A. (2021) · eLife · doi:10.7554/elife.63779 · Cited by 31
Molecular simulation of adsorption properties of thiol-functionalized titanium dioxide (TiO2) nanostructure for heavy metal ions removal from aqueous solution
Chen, B., Li, L., Liu, L., & Cao, J. (2021) · Journal of Molecular Liquids · doi:10.1016/j.molliq.2021.118281 · Cited by 27
RETRACTED: Can spike fragments of SARS-CoV-2 induce genomic instability and DNA damage in the guppy, Poecilia reticulate? An unexpected effect of the COVID-19 pandemic
Gonçalves, S. D. O., da Luz, T. M., Silva, A. M., de Souza, S. S., Montalvão, M. F., Guimarães, A. T. B., Ahmed, M. A. I., Araújo, A. P. D. C., Karthi, S., & Malafaia, G. (2022) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2022.153988 · Cited by 27
Retracted
Combining Fluorescent In Situ Hybridization with the Comet Assay for Targeted Examination of DNA Damage and Repair
Shaposhnikov, S. A., Thomsen, P. D., & Collins, A. R. (2010) · Methods in molecular biology · doi:10.1007/978-1-60327-409-8_10 · Cited by 27
Self-citation
Attenuation of KBrO3‐Induced Renal and Hepatic Toxicity By Cloudy Apple Juice In Rat
Kujawska, M., Ignatowicz, E., Ewertowska, M., Adamska, T., Markowski, J., & Jodynis‐Liebert, J. (2012) · Phytotherapy Research · doi:10.1002/ptr.4848 · Cited by 27
Genotoxicity of doxorubicin in F344 rats by combining the comet assay, flow‐cytometric peripheral blood micronucleus test, and pathway‐focused gene expression profiling
Manjanatha, M. G., Bishop, M. E., Pearce, M. G., Kulkarni, R. M., Lyn‐Cook, L. E., & Ding, W. (2013) · Environmental and Molecular Mutagenesis · doi:10.1002/em.21822 · Cited by 27
Genotoxic Effects in Erythrocytes of Oreochromis niloticus Exposed to Nanograms-per-Liter Concentration of 17β-Estradiol (E2): An Assessment Using Micronucleus Test and Comet Assay
Sponchiado, G., Reynaldo, E. M. F. D. L., de Andrade, A. C. B., de Vasconcelos, E. C., Adam, M. L., & de Oliveira, C. M. R. (2010) · Water Air & Soil Pollution · doi:10.1007/s11270-010-0649-9 · Cited by 27
Evaluation of the protective effect of Ilex paraguariensis and Camellia sinensis extracts on the prevention of oxidative damage caused by ultraviolet radiation
Barg, M., Rezin, G. T., Leffa, D. D., Balbinot, F., Gomes, L. M., Carvalho‐Silva, M., Vuolo, F. S., Petronilho, F. C., Dal‐Pizzol, F., STRECK, E. L., et al. (2013) · Environmental Toxicology and Pharmacology · doi:10.1016/j.etap.2013.11.028 · Cited by 27
Certain Imidazotetrazines Escape O6-Methylguanine-DNA Methyltransferase and Mismatch Repair
Zhang, J., Stevens, M. F. G., Hummersone, M., Madhusudan, S., Laughton, C. A., & Bradshaw, T. D. (2011) · Oncology · doi:10.1159/000327837 · Cited by 27
A Rapid Assay for Measuring Nucleotide Excision Repair by Oligonucleotide Retrieval
Shen, J.-C., Fox, E., Ahn, E. H., & Loeb, L. A. (2014) · Scientific Reports · doi:10.1038/srep04894 · Cited by 27
DNA damage induced by industrial solid waste leachates in Drosophila melanogaster: A mechanistic approach
Siddique, H. R., Sharma, A., Gupta, S. C., Murthy, R. C., Dhawan, A., Saxena, D. K., & Chowdhuri, D. K. (2008) · Environmental and Molecular Mutagenesis · doi:10.1002/em.20373 · Cited by 27
Detection of xenobiotic-induced DNA damage by the comet assay applied to human and rat precision-cut liver slices
Plazar, J., Hreljac, I., Pirih, P., Filipič, M., & Groothuis, G. M. M. (2007) · Toxicology in Vitro · doi:10.1016/j.tiv.2007.02.012 · Cited by 27
Cytogenetic toxicity and gonadal effects of 17 α-methyltestosterone in Astyanax bimaculatus (Characidae) and Oreochromis niloticus (Cichlidae)
Rivero-Wendt, C. L. G., Miranda‐Vilela, A. L., Ferreira, M. F. N., Borges, A. M., & Grisólia, C. K. (2013) · Genetics and Molecular Research · doi:10.4238/2013.september.23.4 · Cited by 27
Evidences of DNA and chromosomal damage induced by the mancozeb-based fungicide Mancozan® in fish (Anguilla anguilla L.)
Marques, A., Rego, A., Guilherme, S., Gaivão, I., Santos, M. A., & Pacheco, M. (2016) · Pesticide Biochemistry and Physiology · doi:10.1016/j.pestbp.2016.03.004 · Cited by 27
Folate deficiency facilitates recruitment of upstream binding factor to hot spots of DNA double-strand breaks of rRNA genes and promotes its transcription
Xie, Q., Li, C., Song, X., Wu, L., Jiang, Q., Qiu, Z., Cao, H., Yu, K., Wan, C., Li, J., et al. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw1208 · Cited by 27
Characterization of Adipose-Derived Stem Cells Following Burn Injury
Prasai, A., Ayadi, A. E., Mifflin, R. C., Wetzel, M. D., Andersen, C. R., Redl, H. R., Herndon, D. N., & Finnerty, C. C. (2017) · Stem Cell Reviews and Reports · doi:10.1007/s12015-017-9721-9 · Cited by 29
Chemopreventive Activities of Sulforaphane and Its Metabolites in Human Hepatoma HepG2 Cells
Liu, P., Wang, W., Zhou, Z., Smith, A. J. O., Bowater, R. P., Wormstone, I. M., Chen, Y., & Bao, Y. (2018) · Nutrients · doi:10.3390/nu10050585 · Cited by 27
Gliadin effect on the oxidative balance and DNA damage: An in-vitro, ex-vivo study
Monguzzi, E., Marabini, L., Elli, L., Vaira, V., Ferrero, S., Ferretti, F., Branchi, F., Gaudioso, G., Scricciolo, A., Lombardo, V., et al. (2018) · Digestive and Liver Disease · doi:10.1016/j.dld.2018.06.020 · Cited by 27
Comet assay for assessment of DNA damage in greenhouse workers exposed to pesticides
Çayır, A., Çoşkun, M., Çoşkun, M., & Çobanoğlu, H. (2019) · Biomarkers · doi:10.1080/1354750x.2019.1610498 · Cited by 27
Detection of Alu sequences and mtDNA in comets using padlock probes
Shaposhnikov, S. A. (2006) · Mutagenesis · doi:10.1093/mutage/gel022 · Cited by 27
Indigo dyes: Toxicity, teratogenicity, and genotoxicity studies in zebrafish embryos
Porkodi, M., Brahmane, M. P., Pathan, M. A., Poojary, N., Singh, S., Harshavarthini, M., & Nagpure, N. S. (2024) · Mutation Research/Genetic Toxicology and Environmental Mutagenesis · doi:10.1016/j.mrgentox.2024.503752 · Cited by 26
Epigenetic and Genotoxic Mechanisms of PFAS-Induced Neurotoxicity: A Molecular and Transgenerational Perspective
Kebieche, N., Yim, S., Lambert, C., & Soulimani, R. (2025) · Toxics · doi:10.3390/toxics13080629 · Cited by 29
Diet carotenoid lutein modulates the expression of genes related to oxygen transporters and decreases DNA damage and oxidative stress in mice
Serpeloni, J. M., Cólus, I. M. D. S., Oliveira, F. S., Aissa, A. F., Mercadante, A. Z., Bianchi, M. L. P., & Antunes, L. M. G. (2014) · Food and Chemical Toxicology · doi:10.1016/j.fct.2014.05.018 · Cited by 26
N-nitroso-N-ethylurea activates DNA damage surveillance pathways and induces transformation in mammalian cells
Bodakuntla, S., Anandi, L., V, Sural, S., Trivedi, P., & Lahiri, M. (2014) · BMC Cancer · doi:10.1186/1471-2407-14-287 · Cited by 26
Changes in expression profiles of genes associated with DNA repair following induction of DNA damage in larval zebrafish Danio rerio
Reinardy, H. C., Dharamshi, J. E., Jha, A. N., & Henry, T. B. (2013) · Mutagenesis · doi:10.1093/mutage/get038 · Cited by 26
Genomic damage as a biomarker of chronic kidney disease status
Corredor, Z., Stoyanova, E., Rodríguez‐Ribera, L., Coll, E., Silva, I., Díaz, J. M., Ballarín, J., Marcos, R., & Pastor, S. (2014) · Environmental and Molecular Mutagenesis · doi:10.1002/em.21911 · Cited by 26
Sinuleptolide inhibits proliferation of oral cancer Ca9-22 cells involving apoptosis, oxidative stress, and DNA damage
Chang, Y., Huang, C., Li, K.-T., Li, R., Liaw, C., Wu, S., Liu, J., Sheu, J., & Chang, H. (2016) · Archives of Oral Biology · doi:10.1016/j.archoralbio.2016.02.019 · Cited by 26
Exposure to sorbitol during lactation causes metabolic alterations and genotoxic effects in rat offspring
Cardoso, F. S., Araújo-Lima, C. F., Aiub, C. A. F., & Felzenszwalb, I. (2016) · Toxicology Letters · doi:10.1016/j.toxlet.2016.08.018 · Cited by 26
Cisplatin resistance in cell models: evaluation of metallomic and biological predictive biomarkers to address early therapy failure
Espina, M., Corte‐Rodríguez, M., Aguado, L., Montes‐Bayón, M., Sierra, M., Martínez‐Camblor, P., Blanco‐González, E., & Sierra, L. M. (2017) · Metallomics · doi:10.1039/c7mt00014f · Cited by 26
Genotoxicity of gemfibrozil in the gilthead seabream (Sparus aurata)
Barreto, Â., Luis, L. G., Soares, A. M. V. M., Paíga, P., Santos, L. H. M. L. M., Delerue-Matos, C. M., Hylland, K., Loureiro, S., & Oliveira, M. (2017) · Mutation Research/Genetic Toxicology and Environmental Mutagenesis · doi:10.1016/j.mrgentox.2017.05.011 · Cited by 26
Mobile phone specific electromagnetic fields induce transient DNA damage and nucleotide excision repair in serum-deprived human glioblastoma cells
Al-Serori, H., Ferk, F., Kundi, M., Bileck, A., Gerner, C., Mišík, M., Nersesyan, A. K., Waldherr, M., Murbach, M., Lah, T. T., et al. (2018) · PLoS ONE · doi:10.1371/journal.pone.0193677 · Cited by 26
Self-citation
Exposure characterization and estimation of benchmark dose for cancer biomarkers in an occupational cohort of diesel engine testers
Niu, Y., Xiao, Z., Meng, T., Wang, H., Bin, P., Shen, M., Chen, W. L., Yu, S., Leng, S., & Zheng, Y. (2018) · Journal of Exposure Science & Environmental Epidemiology · doi:10.1038/s41370-018-0061-x · Cited by 26
Metabolic signatures of germination triggered by kinetin in Medicago truncatula
Araújo, S. D. S., Pagano, A., Dondi, D., Lazzaroni, S., Pinela, E., Macovei, A., & Balestrazzi, A. (2019) · Scientific Reports · doi:10.1038/s41598-019-46866-6 · Cited by 26
Environmental exposure to mineral coal and by-products: Influence on human health and genomic instability
de Souza, M. R., Garcia, A. L. H., Dalberto, D., Martins, G. D. S., Picinini, J., de Souza, G. M. S., Chytry, P., Dias, J. F., Bobermin, L. D., Quincozes‐Santos, A., et al. (2021) · Environmental Pollution · doi:10.1016/j.envpol.2021.117346 · Cited by 26
Farmers exposed to pesticides have almost five times more DNA damage: a meta-analysis study
Nascimento, F. D. A., Silva, D. D. M. E., Pedroso, T. M. A., Ramos, J. S. A., & Parise, M. R. (2021) · Environmental Science and Pollution Research · doi:10.1007/s11356-021-15573-z · Cited by 26
Toxicological Comparison of Mancozeb and Zoxamide Fungicides at Environmentally Relevant Concentrations by an In Vitro Approach
Lori, G., Tassinari, R., Narciso, L., Udroiu, I., Sgura, A., Maranghi, F., & Tait, S. (2021) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph18168591 · Cited by 26
Effects of 2′,4′-Dihydroxy-6′-methoxy-3′,5′-dimethylchalcone from Syzygium nervosum Seeds on Antiproliferative, DNA Damage, Cell Cycle Arrest, and Apoptosis in Human Cervical Cancer Cell Lines
Utama, K., Khamto, N., Meepowpan, P., Aobchey, P., Kantapan, J., Sringarm, K., Roytrakul, S., & Sangthong, P. (2022) · Molecules · doi:10.3390/molecules27041154 · Cited by 26
Toxicity mechanisms of aflatoxin M1 assisted with molecular docking and the toxicity-limiting role of trans-resveratrol
Güç, İ., Yalçın, E., Çavuşoğlu, K., & Acar, A. (2022) · Scientific Reports · doi:10.1038/s41598-022-18791-8 · Cited by 26
Biological effects of decabromodiphenyl ether (BDE209) and Pb on earthworm (Eisenia fetida) in a soil system
Li, J., Zhang, W., Chen, L., Liang, J., & Lin, K. (2015) · Environmental Pollution · doi:10.1016/j.envpol.2015.09.034 · Cited by 26
Synergism Between Propolis and Hyperthermal Intraperitoneal Chemotherapy with Cisplatin on Ehrlich Ascites Tumor in Mice
Oršolić, N., Car, N., Lisičić, D., Benković, V., Knežević, A. H., Đikić, D., & Petrik, J. (2013) · Journal of Pharmaceutical Sciences · doi:10.1002/jps.23755 · Cited by 26
DNA damage induction and antiproliferative activity of vanadium(V) oxido monoperoxido complex containing two bidentate heteroligands
Andrezálová, L., Gbelcová, H., & Ďuračková, Z. (2012) · Journal of Trace Elements in Medicine and Biology · doi:10.1016/j.jtemb.2012.04.007 · Cited by 26
Evaluation of the nuclear DNA Diffusion Assay to detect apoptosis and necrosis
Gichner, T., Mukherjee, A., Wagner, E. D., & Plewa, M. J. (2005) · Mutation Research/Genetic Toxicology and Environmental Mutagenesis · doi:10.1016/j.mrgentox.2005.05.010 · Cited by 26
Single-walled carbon nanotubes induce cytotoxicity and DNA damage via reactive oxygen species in human hepatocarcinoma cells
Alarifi, S. A., Ali, D., Verma, A., Almajhdi, F. N., & Al-Qahtani, A. A. (2014) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-014-9760-3 · Cited by 26
Evaluation of genotoxicity and cytotoxicity induced by different molecular weights of polyethylenimine/DNA nanoparticles
Gholami, L., Sadeghnia, H. R., Darroudi, M., & Oskuee, R. K. (2014) · TURKISH JOURNAL OF BIOLOGY · doi:10.3906/biy-1309-51 · Cited by 26
Genotoxic Potential of the Insecticide Imidacloprid in a Non-Target Organism (Oreochromis niloticus-Pisces)
Ansoar-Rodríguez, Y., Christofoletti, C. A., Marcato, A. C. D. C., Correia, J. E., Bueno, O. C., Malaspina, O., & Fontanetti, C. S. (2015) · Journal of Environmental Protection · doi:10.4236/jep.2015.612118 · Cited by 27
Analysis of possible genotoxicity of the herbicide flurochloridone and its commercial formulations: Endo III and Fpg alkaline comet assays in Chinese hamster ovary (CHO-K1) cells
Soloneski, S., Nikoloff, N., & Larramendy, M. L. (2016) · Mutation Research/Genetic Toxicology and Environmental Mutagenesis · doi:10.1016/j.mrgentox.2016.01.004 · Cited by 26
Role of Macronutrients and Micronutrients in DNA Damage: Results From a Food Frequency Questionnaire
Ladeira, C., Carolino, E., Gomes, M. C., & Brito, M. (2017) · Nutrition and Metabolic Insights · doi:10.1177/1178638816684666 · Cited by 26
Analysis of DNA Damage via Single-Cell Electrophoresis
Anderson, D. C., & Laubenthal, J. (2013) · Methods in molecular biology · doi:10.1007/978-1-62703-565-1_14 · Cited by 26
Combined effect of Benzophenone-2 and ultraviolet radiation promote photogenotoxicity and photocytotoxicity in human keratinocytes
Amar, S. K., Goyal, S., Srivastav, A. K., Chopra, D., & Ray, R. S. (2018) · Regulatory Toxicology and Pharmacology · doi:10.1016/j.yrtph.2018.04.003 · Cited by 26
An investigation of some Schiff base derivatives as chemosensors for Zn(II): The performance characteristics and potential applications
Ergun, E., Ergun, Ü., İleri, Ö., & Küçükmüzevir, M. F. (2018) · Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy · doi:10.1016/j.saa.2018.05.116 · Cited by 26
Lack of potential carcinogenicity for sucralose – Systematic evaluation and integration of mechanistic data into the totality of the evidence
Chappell, G. A., Borghoff, S. J., Pham, L., Doepker, C. L., & Wikoff, D. S. (2019) · Food and Chemical Toxicology · doi:10.1016/j.fct.2019.110898 · Cited by 26
Genotoxic and cytotoxic properties of two medical plants (Teucrium arduini L.and Teucrium flavum L.) in relation to their polyphenolic contents
Grujičić, D., Marković, A., Vukajlović, J. T., Stanković, M. S., Jakovljević, M. R., Ćirić, A., Djordjević, K., Planojević, N. S., Milutinović, M. G., & Milošević-Djordjević, O. (2020) · Mutation Research/Genetic Toxicology and Environmental Mutagenesis · doi:10.1016/j.mrgentox.2020.503168 · Cited by 26
DNA damage in Spodoptera exigua after multigenerational cadmium exposure - A trade-off between genome stability and adaptation
Augustyniak, M., Tarnawska, M., Dziewięcka, M., Kafel, A., Rost-Roszkowska, M. M., & Babczyńska, A. I. (2020) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2020.141048 · Cited by 25
Genotoxic potential and heart rate disorders in the Mediterranean mussel Mytilus galloprovincialis exposed to Superdispersant-25 and dispersed diesel oil
Martinović, R., Kolarević, S., Kračun‐Kolarević, M., Kostić, J., Marković, S., Gačić, Z. M., Kljajić, Z., & Vuković‐Gačić, B. (2015) · Marine Environmental Research · doi:10.1016/j.marenvres.2015.05.001 · Cited by 26
Sensitive voltammetric detection of DNA damage at carbon electrodes using DNA repair enzymes and an electroactive osmium marker
Havran, L., Vacek, J., Cahová, K., & Fojta, M. (2008) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-008-1850-1 · Cited by 26
Effect of dietary meat and fish on endogenous nitrosation, inflammation and genotoxicity of faecal water
Joosen, A. M. C. P., Lecommandeur, E., Kuhnle, G., Aspinall, S. M., Kap, L., & Rodwell, S. A. (2010) · Mutagenesis · doi:10.1093/mutage/gep070 · Cited by 26
Laser-Induced Radiation Microbeam Technology and Simultaneous Real-Time Fluorescence Imaging in Live Cells
Botchway, S. W., Reynolds, P., Parker, A. W., & O’Neill, P. (2012) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/b978-0-12-391857-4.00001-x · Cited by 26
Trimethyltin chloride induces apoptosis and DNA damage via ROS/NF-κB in grass carp liver cells causing immune dysfunction
Li, L., Gao, M., Yang, N., Ai, L., Guo, L., Xue, X., & Sheng, Z. (2023) · Fish & Shellfish Immunology · doi:10.1016/j.fsi.2023.109082 · Cited by 25
Interaction of Cold Atmospheric Pressure Plasma with Soybean Seeds: Effect on Germination and DNA, Seed Surface Characteristics and Plasma Diagnostics
Tomeková, J., Švubová, R., Slováková, Ľ., Holubová, Ľ., Kyzek, S., Gálová, E., & Zahoranová, A. (2023) · Plasma Chemistry and Plasma Processing · doi:10.1007/s11090-023-10398-9 · Cited by 25
Parental exposure to methyl methane sulfonate of three-spined stickleback: contribution of DNA damage in male and female germ cells to further development impairment in progeny
Santos, R., Ladeiro, M. P., Besnard, A. G., Reggio, J., Vulliet, E., Porcher, J., Bony, S., Sánchez, W., & Devaux, A. (2013) · Ecotoxicology · doi:10.1007/s10646-013-1088-3 · Cited by 25
DNA damage induced by phenylalanine and its analogue p-chlorophenylalanine in blood and brain of rats subjected to a model of hyperphenylalaninemia
Simon, K. R., Santos, R. M. D., Scaini, G., Leffa, D. D., Damiani, A. P., Furlanetto, C. B., Machado, J. L., Cararo, J. H., Macan, T. P., STRECK, E. L., et al. (2013) · Biochemistry and Cell Biology · doi:10.1139/bcb-2013-0023 · Cited by 25
A combined γ-H2AX and 53BP1 approach to determine the DNA damage-repair response to exercise in hypoxia
Williamson, J., Hughes, C., Burke, G. A., & Davison, G. W. (2020) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2020.04.026 · Cited by 25
Immunomodulatory, trypanocide, and antioxidant properties of essential oil fractions of Lippia alba (Verbenaceae)
Quintero, W. L., Moreno, É. M., Pinto, S. M. L., Sanabria, S. M., Stashenko, E. E., & García, L. T. (2021) · BMC Complementary Medicine and Therapies · doi:10.1186/s12906-021-03347-6 · Cited by 25

Authors: Collins, A. R.
Affiliations: University of Oslo (NO)
Corresponding author: Collins, Andrew R.
ORCID: Collins, Andrew R.
Venue: Molecular Biotechnology
Publisher: Springer Science and Business Media LLC
Published: 2004
Volume: 26
Issue: 3
Pages: 249-261
ISSN: 1073-6085 (ISSN-L)
DOI: 10.1385/mb:26:3:249
OpenAlex: W2034485370
Cited by: 2,892
References: 46
Citations by year: 2026: 91 · 2025: 104 · 2024: 119 · 2023: 134 · 2022: 138 · 2021: 157 · 2020: 146 · 2019: 168 · 2018: 164 · 2017: 174 · 2016: 161 · 2015: 179 · 2014: 156 · 2013: 162 · 2012: 171
FWCI: 27.05
Type: journal article
Language: en
Open access: closed
Locations: Molecular Biotechnology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
Keywords: comet assay, DNA strand breaks, DNA damage, DNA repair, genotoxicity testing, genotoxins, human biomonitoring, molecular epidemiology, DNA supercoiling
MeSH terms: Animals, DNA, DNA Damage, DNA Mutational Analysis, DNA Repair, Environmental Monitoring, Environmental Pollutants, Humans, Mutagens, Apoptosis, Molecular Epidemiology, Comet Assay

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

The Comet Assay for DNA Damage and Repair: Principles, Applications, and Limitations

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks