Large-Scale transfection of mammalian cells for the fast production of recombinant protein

Pham, Phuong Lan, Kamen, Amine, Durocher, Yves·2006·Molecular Biotechnology

Cited by (236)

Items 101–200 of 236

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  6. Optimized Recombinant Production of Secreted Proteins Using Human Embryonic Kidney (HEK293) Cells Grown in Suspension
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  7. Transient gene expression optimization and expression vector comparison to improve HIV-1 VLP production in HEK293 cell lines
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  8. Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells
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  10. Augmenting recombinant antibody production in HEK293E cells: optimizing transfection and culture parameters
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  11. Enhanced plasmid DNA utilization in transiently transfected CHO–DG44 cells in the presence of polar solvents
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  12. When less becomes more: Optimization of protein expression in HEK293–EBNA1 cells using plasmid titration – A case study for NLRs
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  13. PEI-Mediated Transient Transfection of High Five Cells at Bioreactor Scale for HIV-1 VLP Production
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  15. FMD empty capsids combined with the Immunostant Particle Adjuvant -ISPA or ISA206 induce protective immunity against foot and mouth disease virus
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  16. Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells
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  17. Monoclonal Antibody Expression in Mammalian Cells
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  18. Extracellular vesicle depletion and UGCG overexpression mitigate the cell density effect in HEK293 cell culture transfection
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  26. Perfusion Cell Culture Processes for Biopharmaceuticals
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  27. Transient expression of rabies virus glycoprotein (RVGP) in Drosophila melanogaster Schneider 2 (S2) cells
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  30. Effect of addition of ‘carrier’ DNA during transient protein expression in suspension CHO culture
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  33. Large-Scale Production of Recombinant Noggin and R-Spondin1 Proteins Required for the Maintenance of Stem Cells in Intestinal Organoid Cultures
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  39. The significance of chemical transfection/transduction enhancers in promoting the viral vectors-assisted gene delivery approaches: A focus on potentials for inherited retinal diseases
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  41. Intensification of rAAV Production Based on HEK293 Cell Transient Transfection
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  42. Algal autolysate medium to label proteins for NMR in mammalian cells
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  43. Generation of a recombinant chickenized monoclonal antibody against the neuraminidase of H9N2 avian influenza virus
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  44. Manufacture of Recombinant Therapeutic Proteins Using Chinese Hamster Ovary Cells in Large‐Scale Bioreactors
    Florian Maria Wurm, Maria de Jesus · 2016
  45. Development of novel lipoplex formulation methodologies to improve large-scale transient transfection for lentiviral vector manufacture
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  46. Roles of the Linker Region of RNA Helicase A in HIV-1 RNA Metabolism
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  47. Combined TGE-SGE Expression of Novel PAI-1-Resistant t-PA in CHO DG44 Cells Using Orbitally Shaking Disposable Bioreactors
    Fatemeh Davami · 2011 · Journal of Microbiology and Biotechnology
  48. Adeno-Associated Virus Type 2 Induces Apoptosis in Human Papillomavirus-Infected Cell Lines but Not in Normal Keratinocytes
    Samina Alam, Craig Meyers · 2009 · Journal of Virology
  49. Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures
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  50. Internalization of PEI-based complexes in transient transfection of HEK293 cells is triggered by coalescence of membrane heparan sulfate proteoglycans like Glypican-4
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  51. Development of an alternating tangential flow (ATF) perfusion‐based transient gene expression (TGE) bioprocess for universal influenza vaccine
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  52. Promoter CAG is more efficient than hepatocyte‑targeting TBG for transgene expression via rAAV8 in liver tissues
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  53. Development of apoptosis-resistant CHO cell line expressing PyLT for the enhancement of transient antibody production
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  54. Optimization of gene delivery to HEK293T cells by microporation using a central composite design methodology
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  55. Gadd45-induced cell cycle G2/M arrest for improved transient gene expression in Chinese hamster ovary cells
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  56. Lentiviral expression system for the purification of secreted proteins from human cell cultures
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  57. Comparison of different transient gene expression systems for the production of a new humanized anti-HER2 monoclonal antibody (Hersintuzumab)
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  62. The role of A-kinase anchoring protein 95-like protein in annealing of tRNALys3to HIV-1 RNA
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