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Virulence genes detection of Salmonella serovars isolated from pork and slaughterhouse environment in Ahmedabad, Gujarat
Open accessAbstract
AIM: The aim was to detect virulence gene associated with the Salmonella serovars isolated from pork and Slaughterhouse environment. MATERIALS AND METHODS: Salmonella isolates (n=37) used in this study were isolated from 270 pork and slaughter house environmental samples collected from the Ahmedabad Municipal Corporation Slaughter House, Ahmedabad, Gujarat, India. Salmonella serovars were isolated and identified as per BAM USFDA method and serotyped at National Salmonella and Escherichia Centre, Central Research Institute, Kasauli (Himachal Pradesh, India). Polymerase chain reaction technique was used for detection of five genes, namely invA, spvR, spvC, fimA and stn among different serovars of Salmonella. RESULTS: Out of a total of 270 samples, 37 (13.70%) Salmonella were isolated with two serovars, namely Enteritidis and Typhimurium. All Salmonella serovars produced 284 bp invA gene, 84 bp fimA and 260 bp amplicon for enterotoxin (stn) gene whereas 30 isolates possessed 310 bp spvR gene, but no isolate possessed spvC gene. CONCLUSION: Presence of invA, fimA and stn gene in all isolates shows that they are the specific targets for Salmonella identification and are capable of producing gastroenteric illness to humans, whereas 20 Typhimurium serovars and 10 Enteritidis serovars can able to produce systemic infection.
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“This frequency of spvR is significantly higher than those reported by several authors [24, 31, 48].”
From Characterization of virulence factors of Salmonella isolated from human stools and street food in urban areas of Burkina Faso (2021) · cites this paper as [31]
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“Elsewhere, published papers [31, 46–48] reveal that invA gene has already been detected in 100% of Salmonella strains.”
From Characterization of virulence factors of Salmonella isolated from human stools and street food in urban areas of Burkina Faso (2021) · cites this paper as [31]
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Nikiema, M. E. M., Kakou-ngazoa, S., Ky/Ba, A., Sylla, A., Bako, E., Addablah, A. Y. A., Ouoba, J. B., Sampo, E., Gnada, K., Zongo, O., et al. (2021) · BMC Microbiology · doi:10.1186/s12866-021-02398-6 · Cited by 47
Metadata
- Authors
- Chaudhary, J. H., Nayak, J. B., Brahmbhatt, M. N., & Makwana, P. P.
- Affiliations
- Anand Agricultural University (IN)
- Corresponding author
- Chaudhary, J. H.
- ORCID
- Chaudhary, J. H.
- Venue
- Veterinary World
- Publisher
- Veterinary World
- Published
- 2015-01
- Volume
- 8
- Issue
- 1
- Pages
- 121-124
- ISSN
- 0972-8988 (ISSN-L)
- 2231-0916
- PMC
- PMC4777800
- OpenAlex
- W1972654157
- Cited by
- 77
- References
- 0
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 13 · 2023: 11 · 2022: 15 · 2021: 11 · 2020: 6 · 2019: 5 · 2018: 5 · 2017: 3 · 2016: 1 · 2015: 1
- FWCI
- 4.45
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Veterinary World (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Salmonella and Campylobacter epidemiology
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Life Sciences › Immunology and Microbiology › Microbiology › Enterobacteriaceae and Cronobacter Research
- Keywords
- Salmonella, virulence genes, pork, slaughterhouse environment, Salmonella serotypes, Salmonella Enteritidis, Salmonella typhimurium, invA gene, fimA gene, stn gene, foodborne pathogens, Ahmedabad
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