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Equid herpesvirus type 1 (EHV-1) disrupts actin cytoskeleton during productive infection in equine leukocytes
Open accessAbstract
Equid herpesvirus type 1 (EHV-1) is a prevalent causative agent of equine diseases worldwide. After primary replication in the respiratory epithelium the virus disseminates systemically through a peripheral blood mononuclear cell (PBMC)-associated viraemia. EHV-1 is the only alphaherpes- virus known so far which is capable of establishing latent infection not only in neurons but also in immune system cells (mainly in lymphocytes and macrophages). Since leukocytes are not the target cells for viral replication but are used to transport EHV-1 to the internal organs, the questionremains how the virus avoids the immune response and whether it could potentially be associated with virus-induced cytoskeletal rearrangements. Therefore, the aim of this study was to investigate the progress of EHV-1 replication in leukocytes stimulated by phytohemagglutinin and the impact of EHV-1 infection on the actin cytoskeleton. Using the real-time PCR method we evaluated the quantity of viral DNA from samples collected at indicated time points post infection. In order to examine possible changes in actin cytoskeleton organization due to EHV-1 infection, we performed immunofluorescent staining using TRITC-phalloidin conjugate. The results showed that EHV-1 rep- licates in leukocytes at a restricted level but with the accompaniment of chromatin degradation. Simultaneously, infection with EHV-1 caused disruption of the actin cytoskeleton; this was particularly apparent in further stages of infection. Disruption of the actin cytoskeleton may lead to the limited release of the virus from the cells, but may be also beneficial for the virus, since at the same time it potentially impairs the immune function of leukocytes.
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“Most remarkably, no EHV-1-productive infection in PBMC was observed, which, however, does not exclude unambiguously restricted productive virus replication albeit at low levels (Drebert et al., 2015; Laval et al., 2015; Spiesschaert et al., 2015a).”
From Equid Herpesvirus-1 Exploits the Extracellular Matrix of Mononuclear Cells to Ensure Transport to Target Cells (2020) · cites this paper as Drebert et al., 2015
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Metadata
- Authors
- Drebert, Z., Golke, A., Cymerys, J., Słońska, A., Chmielewska, A., Tucholska, A., & Bańbura, M. W.
- Affiliations
- Warsaw University of Life Sciences (PL)
- Corresponding author
- Golke, A.
- Venue
- Polish Journal of Veterinary Sciences
- Publisher
- Walter de Gruyter GmbH
- Published
- 2015-03-01
- Volume
- 18
- Issue
- 1
- Pages
- 107-112
- ISSN
- 1505-1773 (ISSN-L)
- 2300-2557
- OpenAlex
- W2010126661
- Cited by
- 10
- References
- 25
- Citations by year
- 2021: 1 · 2020: 2 · 2018: 2 · 2017: 4 · 2016: 1
- FWCI
- 1.83
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Polish Journal of Veterinary Sciences (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion, other-oa)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Herpesvirus Infections and Treatments
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Health Sciences › Medicine › Oncology › Viral-associated cancers and disorders
- Keywords
- actin cytoskeleton, productive infection, peripheral blood mononuclear cells, viral replication, chromatin degradation, alphaherpesvirus, immune evasion, viremia, phytohemagglutinin stimulation
- MeSH terms
- Animals, Cells, Cultured, Herpesvirus 1, Equid, Horses, Leukocytes, Actin Cytoskeleton, Mitogens, Virus Replication
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