The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Characterization of New MiniSTR Loci to Aid Analysis of Degraded DNA
Abstract
A number of studies have demonstrated that successful analysis of degraded DNA specimens from mass disasters or forensic evidence improves with smaller sized polymerase chain reaction (PCR) products. We have scanned the literature for new STR loci, unlinked from the CODIS markers, which can generate amplicons less than 125 bp in size and would therefore be helpful in testing degraded DNA samples. New PCR primers were designed and tested for the STR loci D1S1677, D2S441, D4S2364, D10S1248, D14S1434, and D22S1045, arranged into two miniSTR triplexes. All loci show a moderate degree of polymorphism among 474 U.S. population samples tested and were reliable and sensitive to at least 100 pg of DNA template under controlled laboratory conditions and pristine DNA samples. The utility of these new loci were confirmed in comparing the success of the miniSTR assays for typing degraded bone samples while partial profiles were observed with the majority of the samples using a commercial STR kit.
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Cites
Reliable genotyping of samples with very low DNA quantities using PCR
Taberlet, P. (1996) · Nucleic Acids Research · doi:10.1093/nar/24.16.3189 · Cited by 1,491
Cited by
Genetics and Genomics of Core Short Tandem Repeat Loci Used in Human Identity Testing
Butler, J. M. (2006) · Journal of Forensic Sciences · doi:10.1111/j.1556-4029.2006.00046.x · Cited by 686
Self-citationSTR allele sequence variation: Current knowledge and future issues
Gettings, K. B., Aponte, R. A., Vallone, P. M., & Butler, J. M. (2015) · Forensic Science International Genetics · doi:10.1016/j.fsigen.2015.06.005 · Cited by 205
Self-citationHill, C. R., Duewer, D. L., Kline, M. C., Sprecher, C. J., McLaren, R. S., Rabbach, D. R., Krenke, B. E., Ensenberger, M. G., Fulmer, P. M., Storts, D. R., et al. (2011) · Forensic Science International: Genetics · doi:10.1016/j.fsigen.2010.03.014 · Cited by 109
Self-citationBerger, B., Berger, C., Hecht, W., Hellmann, A., Rohleder, U., Schleenbecker, U., & Parson, W. (2014) · Forensic Science International: Genetics · doi:10.1016/j.fsigen.2013.07.002 · Cited by 46
Abboosh, T. S., Kassab, A. C., Al-Dogmi, A. M., Safhi, F. A., Alshehri, E., Alotaibi, A. M., & Al-Qahtani, W. S. (2023) · Forensic Science International · doi:10.1016/j.forsciint.2023.111684 · Cited by 2
Metadata
- Authors
- Coble, M., & Butler, J.
- Venue
- Journal of Forensic Sciences
- Publisher
- ASTM International
- Published
- 2005-01-01
- Volume
- 50
- Issue
- 1
- Pages
- JFS2004216-11
- ISSN
- 0022-1198 (ISSN-L)
- OpenAlex
- W2002883926
- Cited by
- 322
- References
- 1
- FWCI
- 8.59
- Type
- journal article
- Language
- en
- Open access
- closed
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