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Differential Regulation of Gonadotropin-Releasing Hormone Neuron Activity and Membrane Properties by Acutely Applied Estradiol: Dependence on Dose and Estrogen Receptor Subtype
Open accessAbstract
Gonadotropin-releasing hormone (GnRH) neurons are critical to controlling fertility.In vivo, estradiol can inhibit or stimulate GnRH release depending on concentration and physiological state. We examined rapid, nongenomic effects of estradiol. Whole-cell recordings were made of GnRH neurons in brain slices from ovariectomized mice with ionotropic GABA and glutamate receptors blocked. Estradiol was bath applied and measurements completed within 15 min. Estradiol from high physiological (preovulatory) concentrations (100 p m) to 100 n m enhanced action potential firing, reduced afterhyperpolarizing potential (AHP) and increased slow afterdepolarization amplitudes (ADP), and reduced IAHP and enhanced IADP. The reduction of IAHP was occluded by previous blockade of calcium-activated potassium channels. These effects were mimicked by an estrogen receptor (ER) β-specific agonist and were blocked by the classical receptor antagonist ICI182780. ERα or GPR30 agonists had no effect. The acute stimulatory effect of high physiological estradiol on firing rate was dependent on signaling via protein kinase A. In contrast, low physiological levels of estradiol (10 p m) did not affect intrinsic properties. Without blockade of ionotropic GABA and glutamate receptors, however, 10 p m estradiol reduced firing of GnRH neurons; this was mimicked by an ERα agonist. ERα agonists reduced the frequency of GABA transmission to GnRH neurons; GABA can excite to these cells. In contrast, ERβ agonists increased GABA transmission and postsynaptic response. These data suggest rapid intrinsic and network modulation of GnRH neurons by estradiol is dependent on both dose and receptor subtype. In cooperation with genomic actions, nongenomic effects may play a role in feedback regulation of GnRH secretion.
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“E2 at high physiological concentrations enhance GnRH firing frequency through modulation of the after-hyperpolarization potential via a membrane-mediated ERβ-PKA pathway in mice41.”
From Emerging insights into hypothalamic‐pituitary‐gonadal axis regulation and interaction with stress signalling (2018) · cites this paper as [41]
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Metadata
- Authors
- Chu, Z., Andrade, J., Shupnik, M. A., & Moenter, S. M.
- Affiliations
- University of Virginia (US)
- ORCID
- Moenter, Suzanne M.
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2009-04-29
- Volume
- 29
- Issue
- 17
- Pages
- 5616-5627
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC2744362
- OpenAlex
- W2009302214
- Cited by
- 133
- References
- 87
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 7 · 2020: 6 · 2019: 4 · 2018: 11 · 2017: 6 · 2016: 10 · 2015: 11 · 2014: 9 · 2013: 11 · 2012: 12
- FWCI
- 18.84
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD-34860, HD41469, R29HD034860, R01HD034860, R37HD034860, R01HD041469)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- GnRH neurons, estradiol, non-genomic estrogen signaling, estrogen receptor beta, estrogen receptor alpha, GPR30, action potential firing, afterhyperpolarization, calcium-activated potassium channels, protein kinase A, GABA transmission, glutamatergic neurotransmission, ovariectomized mice
- MeSH terms
- Animals, Dose-Response Relationship, Drug, Drug Administration Schedule, Estradiol, Female, Gonadotropin-Releasing Hormone, Membrane Potentials, Neurons, Receptors, Estrogen, Time Factors, Protein Subunits, Estrogen Receptor alpha, Estrogen Receptor beta, Mice
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