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Reanalysis of P2X7Receptor Expression in Rodent Brain
Open accessAbstract
P2X receptors are cationic-selective ion channels gated by extracellular ATP. There are seven subunits (P2X1-7), the first six of which are expressed throughout the peripheral and central nervous systems. P2X7 receptors are rapidly upregulated and activated as a result of inflammatory stimuli in immune cells, where they act not only as cationic channels but uniquely couple with rapid release of proinflammatory cytokines, cytoskeletal rearrangements, and apoptosis or necrotic cell death. The P2X7 receptor has been termed the cytolytic non-neuronal P2X receptor because it had not been detected in neurons until recently when it has been immunolocalized to several brain regions, particularly the hippocampus, and has been suggested to be involved in presynaptic modulation of transmitter release. Because its expression in brain neurons may have substantial functional implications, we have performed detailed immunocytochemical, immunoblot, and immunoprecipitation studies on brain and non-neuronal tissue using all currently available antibodies. We first examined rats, but staining patterns were inconsistent among antibodies; we therefore studied mice for which there are two P2X7 knock-out mice constructs available, one expressing the LacZ transgene. We found that P2X7 receptor protein is strongly and reliably detected in the submandibular gland and lung of wild-type mice but not in either of the P2X7-/- mice. However, we failed to find evidence for P2X7 receptor protein in hippocampal neurons or their input-output projections. Either the P2X7 protein in the hippocampus is below the limits of detection by the currently available methods or it is not present.
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“Despite being detected in glial and neuronal cells [49,50,51,52,53,54], the activity of the P2X7R was not always investigated and used antibodies lack specificity [49,55,56,57].”
From P2X7 Receptor Signaling in Stress and Depression (2019) · cites this paper as [49]
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Metadata
- Authors
- Sim, J. A., Young, M. T., Sung, H.-Y., North, R. A., & Surprenant, A.
- Affiliations
- University of Sheffield (GB)
- University of Manchester (GB)
- Corresponding author
- Surprenant, Annmarie
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2004-07-14
- Volume
- 24
- Issue
- 28
- Pages
- 6307-6314
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6729549
- OpenAlex
- W2131072721
- Cited by
- 264
- References
- 44
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 3 · 2023: 6 · 2022: 2 · 2021: 5 · 2020: 7 · 2019: 8 · 2018: 12 · 2017: 13 · 2016: 9 · 2015: 6 · 2014: 13 · 2013: 11 · 2012: 11
- FWCI
- 10.78
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- ORCA Online Research @Cardiff (Cardiff University) (acceptedVersion)
- Funders
- Pfizer
- GlaxoSmithKline
- Wellcome Trust
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
- Health Sciences › Medicine › Physiology (medical) › Pain Mechanisms and Treatments
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- P2X7 receptor, P2X receptors, rodent brain, hippocampus, neuronal expression, P2X7 knockout mice, submandibular gland, lung, ATP-gated ion channels, presynaptic modulation, antibody specificity, neuroinflammation
- MeSH terms
- Animals, Antibodies, Monoclonal, Epitopes, Brain Chemistry, Crosses, Genetic, Hippocampus, Lac Operon, Lung, Male, Mice, Inbred C57BL, Mice, Inbred DBA, Nerve Tissue Proteins, Neurons, Organ Specificity, Submandibular Gland, Rats, Wistar, Receptors, Purinergic P2, Mice, Knockout, Immunoprecipitation, Mice, Rats, Receptors, Purinergic P2X7
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