The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Glutathione Peroxidase-Catalase Cooperativity Is Required for Resistance to Hydrogen Peroxide by Mature Rat Oligodendrocytes
Open accessAbstract
Oxidative mechanisms of injury are important in many neurological disorders, including hypoxic-ischemic brain damage. Cerebral palsy after preterm birth is hypothesized to be caused by hypoxic-ischemic injury of developing oligodendrocytes (OLs). Here we examined the developmental sensitivity of OLs to exogenous hydrogen peroxide (H2O2) with stage-specific rat oligodendrocyte cultures. We found that H2O2 itself or that generated by glucose oxidase was more toxic to developing than to mature OLs. Mature OLs were able to degrade H2O2 faster than developing OLs, suggesting that higher antioxidant enzyme activity might be the basis for their resistance. Catalase expression and activity were relatively constant during oligodendrocyte maturation, whereas glutathione peroxidase (GPx) was upregulated with a twofold to threefold increase in its expression and activity. Thus, it appeared that the developmental change in resistance to H2O2 was caused by modulation of GPx but not by catalase expression. To test the relative roles of catalase and GPx in the setting of oxidative stress, we measured enzyme activity in cells exposed to H2O2 and found that H2O2 induced a decrease in catalase activity in developing but not in mature OLs. Inhibition of GPx by mercaptosuccinate led to an increase in the vulnerability of mature OLs to H2O2 as well as a reduction in catalase activity. Finally, H2O2-dependent inactivation of catalase in developing OLs was prevented by the GPx mimic ebselen. These data provide evidence for a key role for GPx-catalase cooperativity in the resistance of mature OLs to H2O2-induced cell death.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“In vitro studies show that oligodendrocytes are susceptible to oxidative stress due to their high metabolic activity and iron content combined with low antioxidant levels (Back et al., 1998; Baud et al., 2004; Fragoso et al., 2004).”
From Redox dysregulation, neuroinflammation, and NMDA receptor hypofunction: A “central hub” in schizophrenia pathophysiology? (2016) · cites this paper as Baud et al., 2004
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Free radicals in biology and medicine
Gutteridge, J. M. C. (1991) · General Pharmacology The Vascular System · doi:10.1016/0306-3623(91)90478-o · Cited by 17,178
Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue.
McCarthy, K. D., & de Vellis, J. (1980) · The Journal of Cell Biology · doi:10.1083/jcb.85.3.890 · Cited by 3,845
Reactive Oxygen Species and the Central Nervous System
Halliwell, B. (1992) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1992.tb10990.x · Cited by 2,947
Wang, H., & Joseph, J. A. (1999) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(99)00107-0 · Cited by 2,355
Self-citationYakes, F. M., & Van Houten, B. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.2.514 · Cited by 1,854
(1964) · The Medical Journal of Australia · doi:10.5694/j.1326-5377.1964.tb115841.x · Cited by 1,411
Neurobiology of Periventricular Leukomalacia in the Premature Infant
Volpe, J. J. (2001) · Pediatric Research · doi:10.1203/00006450-200111000-00003 · Cited by 945
Self-citationBack, S. A., Luo, N. L., Borenstein, N. S., Levine, J. M., Volpe, J. J., & Kinney, H. C. (2001) · Journal of Neuroscience · doi:10.1523/jneurosci.21-04-01302.2001 · Cited by 936
Self-citationSelective Vulnerability of Late Oligodendrocyte Progenitors to Hypoxia–Ischemia
Back, S. A., Han, B. H., Luo, N. L., Chricton, C. A., Xanthoudakis, S., Tam, J., Arvin, K. L., & Holtzman, D. M. (2002) · The Journal of Neuroscience · doi:10.1523/jneurosci.22-02-00455.2002 · Cited by 795
Pigeolet, E., Corbisier, P., Houbion, A., Lambert, D., Michiels, C., Raes, M. J., Zachary, M.-D., & Remacle, J. (1990) · Mechanisms of Ageing and Development · doi:10.1016/0047-6374(90)90078-t · Cited by 747
Back, S. A., Gan, X., Li, Y., Rosenberg, P. A., & Volpe, J. J. (1998) · The Journal of Neuroscience · doi:10.1523/jneurosci.18-16-06241.1998 · Cited by 586
Self-citationAstrocytes protect neurons from hydrogen peroxide toxicity
Desagher, S., Glowinski, J., & Prémont, J. (1996) · Journal of Neuroscience · doi:10.1523/jneurosci.16-08-02553.1996 · Cited by 543
A role for 12-lipoxygenase in nerve cell death caused by glutathione depletion.
Li, Y., Maher, P., & Schubert, D. (1997) · Neuron · doi:10.1016/s0896-6273(00)80953-8 · Cited by 509
Vulnerability of oligodendroglia to glutamate: pharmacology, mechanisms, and prevention
Oka, A., Belliveau, M. J., Rosenberg, P. A., & Volpe, J. J. (1993) · Journal of Neuroscience · doi:10.1523/jneurosci.13-04-01441.1993 · Cited by 504
Self-citationNitrosative and Oxidative Injury to Premyelinating Oligodendrocytes in Periventricular Leukomalacia
Haynes, R. L., Folkerth, R. D., Keefe, R. J., Sung, I., Swzeda, L. I., Rosenberg, P. A., Volpe, J. J., & Kinney, H. C. (2003) · Journal of Neuropathology & Experimental Neurology · doi:10.1093/jnen/62.5.441 · Cited by 454
Self-citationClaiborne, A., & Fridovich, I. (1979) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)50722-5 · Cited by 370
Oshino, N., Chance, B., Sies, H., & Bücher, T. (1973) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(73)90040-4 · Cited by 270
Novel Role of Vitamin K in Preventing Oxidative Injury to Developing Oligodendrocytes and Neurons
Li, J., Lin, J. C., Wang, H., Peterson, J. W., Furie, B. C., Furie, B., Booth, S. L., Volpe, J. J., & Rosenberg, P. A. (2003) · Journal of Neuroscience · doi:10.1523/jneurosci.23-13-05816.2003 · Cited by 250
Self-citationDitelberg, J. S., Sheldon, R. A., Epstein, C. J., & Ferriero, D. M. (1996) · Pediatric Research · doi:10.1203/00006450-199602000-00003 · Cited by 193
Fullerton, H. J., Ditelberg, J. S., Chen, S. F., Sarco, D. P., Chan, P. H., Epstein, C. J., & Ferriero, D. M. (1998) · Annals of Neurology · doi:10.1002/ana.410440311 · Cited by 185
Selenium-dependent and non-selenium-dependent glutathione peroxidases in human tissue extracts
Carmagnol, F., Sinet, P. M., & Jerome, H. (1983) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/0304-4165(83)90188-5 · Cited by 183
Dringen, R., & Hamprecht, B. (1997) · Brain Research · doi:10.1016/s0006-8993(97)00233-3 · Cited by 182
Brain injury in the premature infant – from pathogenesis to prevention
Volpe, J. J. (1997) · Brain and Development · doi:10.1016/s0387-7604(97)00078-8 · Cited by 172
Self-citationKinetic mechanism and substrate specificity of glutathione peroxidase activity of ebselen (PZ51)
Maiorino, M., Roveri, A., Coassin, M., & Ursini, F. (1988) · Biochemical Pharmacology · doi:10.1016/0006-2952(88)90591-6 · Cited by 168
Superoxide dismutase, glutathione peroxidase and catalase in developing rat brain
Mavelli, I., Rigo, A., Federico, R., Ciriolo, M. R., & Rotilio, G. (1982) · Biochemical Journal · doi:10.1042/bj2040535 · Cited by 147
Reversible inhibition and irreversible inactivation of catalase in presence of hydrogen peroxide
Lardinois, O. M., Mestdagh, M. M., & Rouxhet, P. G. (1996) · Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology · doi:10.1016/0167-4838(96)00043-x · Cited by 146
SUBCELLULAR DISTRIBUTION OF d ‐AMINO ACID OXIDASE AND CATALASE IN RAT BRAIN
Gaunt, G. L., & Duve, C. D. (1976) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1976.tb04447.x-i1 · Cited by 144
Catalase Mediates Acetaldehyde Formation from Ethanol in Fetal and Neonatal Rat Brain
Hamby‐Mason, R., Chen, J. J., Schenker, S., Perez, A., & Henderson, G. I. (1997) · Alcoholism Clinical and Experimental Research · doi:10.1111/j.1530-0277.1997.tb04255.x · Cited by 132
Regional Distribution of Catalase in the Adult Rat Brain
Brannan, T. S., Maker, H. S., & Raes, I. (1981) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1981.tb02411.x · Cited by 129
Yonezawa, M., Back, S. A., Gan, X., Rosenberg, P. A., & Volpe, J. J. (1996) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1996.67020566.x · Cited by 125
Self-citationMarshall, L., Henze, D. A., Hirase, H., Leinekugel, X., Dragoi, G., & Buzsáki, G. (2002) · Journal of Neuroscience · doi:10.1523/jneurosci.22-02-j0001.2002 · Cited by 118
REDUCTION OF γ‐AMINOBUTYRIC ACID LEVEL IN THE CAT CEREBRAL CORTEX BY AFFERENT ELECTRICAL STIMULATION
Lichtshtcin, D., & Dobkin, J. (1976) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1976.tb04448.x · Cited by 113
Immunocytochemical localization of catalase in the central nervous system of the rat.
Moreno, S., Mugnaini, E., & Cerù, M. (1995) · Journal of Histochemistry & Cytochemistry · doi:10.1177/43.12.8537642 · Cited by 112
Distribution of superoxide dismutase, glutathione peroxidase and catalase in developing rat brain
Maestro, R. F. D., & McDonald, W. (1987) · Mechanisms of Ageing and Development · doi:10.1016/0047-6374(87)90051-0 · Cited by 101
Oligodendroglial cell death induced by oxygen radicals and its protection by catalase
Kim, Y. S., & Kim, S. U. (1991) · Journal of Neuroscience Research · doi:10.1002/jnr.490290111 · Cited by 99
Selenium–Glutathione Peroxidase: Properties and Synthesis
Tappel, A. L. (1984) · Current topics in cellular regulation · doi:10.1016/b978-0-12-152824-9.50015-0 · Cited by 96
A new Alamar Blue viability assay to rapidly quantify oligodendrocyte death
Back, S. A., Khan, R., Gan, X., Rosenberg, P. A., & Volpe, J. J. (1999) · Journal of Neuroscience Methods · doi:10.1016/s0165-0270(99)00062-x · Cited by 86
Self-citationGLUTATHIONE PEROXIDASE LEVELS IN BRAIN
de Marchena, O., Guarnieri, M., & McKhann, G. M. (1974) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1974.tb04293.x · Cited by 82
Hirrlinger, J., Resch, A., Gutterer, J. M., & Dringen, R. (2002) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2002.00999.x · Cited by 81
Delgado‐Esteban, M., Almeida, Á., & Bolaños, J. P. (2000) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2000.0751618.x · Cited by 81
Brand, A., Gil, S., Seger, R., & Yavin, E. (2001) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2001.00085.x · Cited by 50
A possible relationship between concentration of microperoxisomes and myelination
Adamo, A. M., Aloise, P. A., & Pasouini, J. M. (1986) · International Journal of Developmental Neuroscience · doi:10.1016/0736-5748(86)90003-1 · Cited by 40
Properties of catalase purified from a methanol‐grown yeast, Kloeckera sp. 2201
Mozaffar, S., Ueda, M., Kitatsuji, K., Shimizu, S., Osumi, M., & Tanaka, A. (1986) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1986.tb09520.x · Cited by 33
Соколова, Т. В., Gutterer, J. M., Hirrlinger, J., Hamprecht, B., & Dringen, R. (2001) · Neuroscience Letters · doi:10.1016/s0304-3940(00)01689-x · Cited by 31
PU.1 regulates glutathione peroxidase expression in neutrophils
Throm, S. L., & Klemsz, M. J. (2003) · Journal of Leukocyte Biology · doi:10.1189/jlb.0203061 · Cited by 19
Isolation and Biochemical Characterization of Peroxisomes from Cultured Rat Glial Cells
Singh, I., Carillo, O., & Namboodiri, A. M. A. (2000) · Neurochemical Research · doi:10.1023/a:1007563201595 · Cited by 13
Flagged first, then by citations
Research works printed but not linked: 2
- 1984, Methods of enzymatic analysis, 3, 273
- 1996, Pediatr Res, 39, 204
Cited by
Sorted by citations
Ferriero, D. M. (2004) · New England Journal of Medicine · doi:10.1056/nejmra041996 · Cited by 1,148
Singh, N., Savanur, M. A., Srivastava, S., D’Silva, P., & Mugesh, G. (2017) · Angewandte Chemie International Edition · doi:10.1002/anie.201708573 · Cited by 665
Tomlinson, D. R., & Gardiner, N. J. (2007) · Nature reviews. Neuroscience · doi:10.1038/nrn2294 · Cited by 578
White matter injury in the preterm infant: pathology and mechanisms
Back, S. A. (2017) · Acta Neuropathologica · doi:10.1007/s00401-017-1718-6 · Cited by 473
Shields, H. J., Traa, A., & Van Raamsdonk, J. M. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.628157 · Cited by 478
Metadata
- Authors
- Baud, O., Greene, A. E., Li, J., Wang, H., Volpe, J. J., & Rosenberg, P. A.
- Affiliations
- Boston Children's Hospital (US)
- Corresponding author
- Rosenberg, Paul A.
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2004-02-18
- Volume
- 24
- Issue
- 7
- Pages
- 1531-1540
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6730475
- OpenAlex
- W2024367623
- Cited by
- 292
- References
- 46
- Citations by year
- 2026: 4 · 2025: 17 · 2024: 7 · 2023: 12 · 2022: 10 · 2021: 14 · 2020: 14 · 2019: 13 · 2018: 16 · 2017: 17 · 2016: 13 · 2015: 12 · 2014: 15 · 2013: 14 · 2012: 12
- FWCI
- 5.70
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of Neurological Disorders and Stroke (NS38475, P01NS038475)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD18655, P30HD018655)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Neonatal and fetal brain pathology
- Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
- Keywords
- glutathione peroxidase, catalase, hydrogen peroxide, oligodendrocyte maturation, oxidative stress, antioxidant enzymes, catalase inactivation, ebselen, mercaptosuccinic acid, hypoxic-ischemic brain injury, cerebral palsy, oxidative cell death, developmental vulnerability
- MeSH terms
- Animals, Catalase, Cell Differentiation, Cells, Cultured, Drug Resistance, Glutathione Peroxidase, Hydrogen Peroxide, Oligodendroglia, Peroxides, Time Factors, Up-Regulation, Oxidants, Rats, Sprague-Dawley, Apoptosis, Oxidative Stress, In Situ Nick-End Labeling, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
