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TORC1 phosphorylates and inhibits the ribosome preservation factor Stm1 to activate dormant ribosomes
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Abstract
Target of rapamycin complex 1 (TORC1) promotes biogenesis and inhibits the degradation of ribosomes in response to nutrient availability. To ensure a basal supply of ribosomes, cells are known to preserve a small pool of dormant ribosomes under nutrient-limited conditions. However, the regulation of these dormant ribosomes is poorly characterized. Here, we show that upon inhibition of yeast TORC1 by rapamycin or nitrogen starvation, the ribosome preservation factor Stm1 mediates the formation of nontranslating, dormant 80S ribosomes. Furthermore, Stm1-bound 80S ribosomes are protected from proteasomal degradation. Upon nutrient replenishment, TORC1 directly phosphorylates and inhibits Stm1 to reactivate translation. Finally, we find that SERBP1, a mammalian ortholog of Stm1, is likewise required for the formation of dormant 80S ribosomes upon mTORC1 inhibition in mammalian cells. These data suggest that TORC1 regulates ribosomal dormancy in an evolutionarily conserved manner by directly targeting a ribosome preservation factor.
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TORC1 can regulate ribosomal dormancy in an evolutionarily conserved manner by directly targeting a ribosome preservation factor.
“A recent study has found that TORC1 can regulate ribosomal dormancy in an evolutionarily conserved manner by directly targeting a ribosome preservation factor [63].”
From Antifungal persistence: Clinical relevance and mechanisms (2025) · cites this paper as [63]
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Cited by
Sorted by citations
Antifungal persistence: Clinical relevance and mechanisms
Wang, R., Lv, J., Chen, L., Zhao, Y., & Du, H. (2025) · PLoS pathogens · doi:10.1371/journal.ppat.1013456 · Cited by 12
“A recent study has found that TORC1 can regulate ribosomal dormancy in an evolutionarily conserved manner by directly targeting a ribosome preservation factor [63].”
Metadata
- Authors
- Shetty, S., Hofstetter, J., Battaglioni, S., Ritz, D., & Hall, M. N.
- Affiliations
- University of Basel (CH)
- Corresponding author
- Hall, Michael N
- Venue
- The EMBO Journal
- Published
- 2023-01-24
- Volume
- 42
- Issue
- 5
- Pages
- e112344-e112344
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC9975950
- OpenAlex
- W4317829445
- Cited by
- 58
- References
- 2
- Citations by year
- 2026: 22 · 2025: 16 · 2024: 10 · 2023: 9
- FWCI
- 5.08
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- The EMBO Journal (publishedVersion, cc-by-nc-nd)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- edoc (University of Basel) (acceptedVersion)
- edoc (University of Basel) (submittedVersion)
- Funders
- European Molecular Biology Organization
- NCCR RNA and Disease
- Swiss National Science Foundation (179569)
- Louis Jeantet Foundation
- Canton of Basel
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › PI3K/AKT/mTOR signaling in cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- TORC1, 80S ribosome, ribosome biogenesis, ribosome degradation, proteasomal degradation, nitrogen starvation, rapamycin, SERBP1, mTORC1, nutrient limitation, yeast, mammalian cells
- MeSH terms
- Mechanistic Target of Rapamycin Complex 1, Animals, DNA-Binding Proteins, Mammals, Ribosomes, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
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