Influence of antibiotics on indirect organogenesis of Teak
Open accessAbstract
Agrobacterium is the largest method employed to transform woody plants. The bacterium is required to introduce the transgene into the plant nuclear genome. After transferring T-DNA to the plant cell, the bacteria affect plant growth negatively and have to be eliminated from plant tissue culture medium through the use of antibiotics. The effect of different antibiotics (timentin, cefotaxime and carbenicillin) on in vitro shoot regeneration of teak (Tectona grandis L. f.) was compared in hypocotyl, mature cotyledon and cotyledonary segments explants. Timentin and cefotaxime (100-300 mg l-1) did not affect shoot regeneration and the number of shoots per explant. Moreover, at these concentrations, the two antibiotics seem to stimulate shoot regeneration. Carbenicillin at a dosage of 300 mg l-1 as well as cefotaxime and timentin at a dosage of 500 mg l-1 induced abundant calli formation and inhibited regeneration. Our data show that cefotaxime and timentin (300 mg l-1) can be harmless to teak regeneration and can be used as bactericide agents during Agrobacterium-mediated transformation of Tectona grandis. Furthermore, we discuss the effect of antibiotic degradation on plant morphogenesis and its effect on regeneration from different explants.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Several studies have reported phytotoxicity of antimicrobial agents to cell, organ, and tissue cultures in plant species.
“Several studies have reported the phytotoxicity of antimicrobial agents to cell, organ, and tissue cultures in plant species [5,6,7,8,10].”
From A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture (2021) · cites this paper as [8]
Sources of in vitro contamination include culture containers, media, explants, equipment, the environment of the culture room and transfer area, and operating personnel.
“Sources of in vitro contamination include culture containers, media, explants, equipment, the environment of the culture room and transfer area, and operating personnel.”
From A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture (2021) · cites this paper as [8]
The Introduction and Discussion describe eliminating Agrobacterium from plant tissue cultures, and the Plant material and antibiotics section describes sterilizing teak seeds. Our copy does not identify the claimed sources of in vitro contamination; searches for contamination sources, culture-room conditions, and operating personnel found no such passage. (LLM, conf=0.76)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
No references could be resolved yet.
Research works printed but not linked: 28
- Ascough G.D., Novák O., Pěnčík A., Rolčík J., Strnad M., Erwin J.E., Van Staden J., 2009. Hormonal and cell division analyses in Watsonia lepida seedlings. Journal of Plant Physiology 166: 1497-1507. DOI: 10.1016/ j.jplph.2009.03.009. · DOI
- Baghel R.S., Tiwari S., Tripathi M.K., 2008. Comparison of morphogenic and plant regeneration ability of some explants of teak (Tectona grandis Linn. F). Journal of Agricultural Science and Technology 4: 125-136.
- Bosela M.J., 2009. Effects of β-lactam antibiotics, aux- ins, and cytokinins on shoot regeneration from callus cultures of two hybrid aspens, Populus tremuloides x P. tremula and P. x canescens x P. gradidentata. Plant Cell, Tissue and Organ Culture 98: 249-261. DOI: 10.1007/ s11240-009-9555-5. · DOI
- Costa M.G.C., Nogueira F.T.S., Figueira M.L., Otoni W.C., Brommonschenkel S.H., Cecon P.R., 2000. Infl uence of the antibiotic Timentin on plant regeneration of tomato (Lycopersicon esculentum Mill.) cultivars. Plant Cell Reports 19: 327-332. DOI: 10.1007/s002990050021. · DOI
- Fofana I.J., Ofori D., Poitel M., Verhaegen D., 2009. Di- versity and genetic structure of teak (Tectona grandis L.f) in its natural range using DNA microsatellite mark- ers. New Forests 37: 175-195. DOI: 10.1007/s11056- 008-9116-5. · DOI
- Giri C.C., Shyamkumar B., Anjaneyulu B., 2004. Progress in tissue culture, genetic transformation and applica- tions of biotechnology to trees: an overview. Trees 18: 115-135. DOI: 10.1007/s00468-003-0287-6. · DOI
- Gyves E.M., Royani J.I., Rugini E., 2007. Effi cient meth- od of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations. Annals of Forest Science 64: 73-78. DOI: 10.1051/forest:2006090. · DOI
- Katayama N., Takano H., Sugiyama M., Takio S., Sakai A., Tanaka K., Kuroiwa H., Ono K. 2003. Effects of anti- biotics that inhibit the bacterial Peptidoglycan synthesis pathway on moss chloroplast division. Plant and Cell Physiology 44: 776-781. DOI: 10.1093/pcp/pcg096. · DOI
- Kong L., Dai D., Shang Li M.K., Zhang C.X., 2009. Thidi- azuron-induced somatic embryos, their multiplication, maturation, and conversion in Cinnamomum paucifl o- rum Nees (Lauraceae). New Forests 38: 131-142. DOI: 10.1007/s11056-009-9135-x. · DOI
- Kucharska D., Orlikowska T., 2009. Enhancement of in vitro organogenetic capacity of rose by preculture of donor shoots on the medium with thidiazuron. Acta Physiologiae Plantarum 31: 495-500. DOI: 10.1007/ s11738-008-0258-z. · DOI
- Le V.Q., Belles-Isles J., Dusabenyagasani M., Tremblay F.M., 2001. An improved procedure for production of white spruce (Picea glauca) transgenic plants using Agrobacterium tumefaciens. Journal of Experimental Botany 52: 2089-2095.
- Lin J-J., Assad-Garcia N., Kuo J., 1995. Plant hormone effect of antibiotics on the plant transformation effi - ciency of plant tissues by Agrobacterium tumefaciens cells. Plant Science 109: 171-177. DOI: 10.1016/0168- 9452(95)04168-T. · DOI
- Ling H.Q., Kriseleit D., Ganal M.W., 1998. Effect of ticar- cillin/potassium clavulanate on callus growth and shoot regeneration in Agrobacterium-mediated transformation of tomato (Lycopersicon esculentum Mill.). Plant Cell Reports 17: 843-847. DOI: 10.1007/s002990050495. · DOI
- Mamidala P., Nanna R.S., 2009. Effi cient in vitro plant re- generation, fl owering and fruiting of dwarf Tomato cv. Micro-Msk. Plant Omics Journal 2: 98-102.
- Mendes A.F.S., Cidade L.C., de Oliveira M.L.P., Otoni W.C., Soares-Filho W.S., Costa M.G.C., 2009. Evalu- ation of novel beta-lactam antibiotics in comparison to cefotaxime on plant regeneration of Citrus sinensis L. Osb. Plant Cell Tissue Organ Cult 97: 331-336. DOI: 10.1007/ s11240-009-9518-x. · DOI
- Miranda J., Konschuh M.N., Yeung E.C., Chinnappa C.C., 1999. In vitro plantlet regeneration from hypocotyls explants of Stellaria longipes (Caryophyllaceae). Cana- dian Journal of Botany 77: 318-322. DOI: 10.1139/cjb- 77-2-318. · DOI
- Murashige T., Skoog F., 1962. A revised medium for rap- id growth and bioassays with tobacco tissue cultures. Plant Physiology 15: 473-497. DOI: 10.1111/j.1399- 3054.1962.tb08052.x. · DOI
- Murthy B.N.S., Murch S.J., Saxena P.K., 1998. Thidi- azuron: A potent regulator of in vitro plant morphogen- esis. In Vitro Cellular & Developmental Biology 34: 267-275. DOI: 10.1007/BF02822732. · DOI
- Nauerbey B., Billing K., Wyndaele R., 1997. Infl uence of the antibiotic Timentin on plant regeneration com- pared to carbenicillin and cefotaxime in concentrations suitable for elimination of Agrobacterium tumefaciens. Plant Science 123: 169-177. DOI: 10.1016/S0168- 9452(96)04569-4. · DOI
- Paiva Neto V.B., Mota T.R., Otoni W.C., 2003. Direct or- ganogenesis from hypocotyl-derived explants of annat- to (Bixa orellana). Plant Cell, Tissue and Organ Culture 75: 159-167. DOI: 10.1023/A: 1025063906822. · DOI
- Poupin M.J., Arce-Johnson P., 2005. Transgenic trees for a new era. In Vitro Cellular & Developmental Biology- Plant 41: 91-101. DOI: 10.1079/IVP2004587. · DOI
- Recht M.I., Douthwaite S., Puglisi J.D., 1999. Basis for prokaryotic specifi city of action of aminoglycoside antibiotics. The EMBO Journal 18: 3133-3138. DOI: 10.1093/emboj/ 18.11.3133. · DOI
- Shirin F., Rana P.K., Mandal A.K., 2005. In vitro clonal propagation of mature Tectona grandis through axil- lary bud proliferation. Journal of Forest Research 10: 465-469. DOI: 10.1007/s10310-005-0173-8. · DOI
- Souza B.M., Kraus J.E., Endres L., Mercier H., 2003. Re- lationships between endogenous hormonal levels and axillary bud development of Ananas comosus nodal segments. Plant Physiology and Biochemistry 41: 733- 739. DOI: 10.1016/S0981-9428(03)00112-8. · DOI
- Tereso S., Miguel C., Maroco J., Oliveira M.M., 2006. Susceptibility of embryogenic and organogenic tissues of maritime pine (Pinus pinaster) to antibiotics used in Agrobacterium-mediated genetic transformation. Plant Cell, Tissue and Organ Culture 87: 33-40. DOI: 10.1007/ s11240-006-9130-2. · DOI
- Tiwari S.K., Tiwari K.P., Siril E.A., 2002. An improved micropropagation protocol for teak. Plant Cell, Tissue and Organ Culture 71: 1-6. DOI: 10.1023/ A:1016570000846. · DOI
- Wightman F., Lighty D.L., 1982. Identifi cation of phen- ylacetic acid as a natural auxin in the shoots of higher plants. Physiologia Plantarum 55: 17-24. DOI: 10.1111/ j.1399-3054.1982.tb00278.x. · DOI
- Zhi-neng L., Guo-feng L., Fang F., Man-zhu B., 2007. Adventitious shoot regeneration of Platanus aceri- folia Willd. facilitated by Timentin, an antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation. Forest Studies in China 9: 14-18. DOI: 10.1007/s11632-007-0003-5. · DOI
Cited by
Sorted by citations
A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture
Sivanesan, I., Muthu, M., Gopal, J., Tasneem, S., Kim, D.-H., & Oh, J.-W. (2021) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph18052282 · Cited by 27 · see citing works
- Authors
- Tambarussi, E. V., Rogalski, M., Nogueira, F. T. S., Brondani, G. E., De Martin, V. D. F., & Carrer, H.
- Affiliations
- Universidade Federal de São Carlos (BR)
- Universidade Federal de Viçosa (BR)
- Universidade Federal de Mato Grosso (BR)
- Universidade de São Paulo (BR)
- Venue
- Annals of Forest Research
- Published
- 2015-03-09
- Volume
- 58
- Issue
- 1
- Pages
- 177-183
- ISSN
- 1844-8135 (ISSN-L)
- 2065-2445
- OpenAlex
- W2061038620
- Cited by
- 27
- References
- 0
- Citations by year
- 2025: 3 · 2024: 1 · 2023: 3 · 2021: 5 · 2020: 5 · 2019: 4 · 2018: 2 · 2017: 3 · 2016: 1
- FWCI
- 0.87
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Annals of Forest Research (publishedVersion)
- Americanae (AECID Library) (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
- Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Pathogenic Bacteria Studies
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Flowering Plant Growth and Cultivation
- Keywords
- Tectona grandis, Agrobacterium-mediated transformation, antibiotic selection, timentin, cefotaxime, carbenicillin, shoot regeneration, indirect organogenesis, callus formation, explant source, cotyledon explants, hypocotyl explants, bactericides
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
