Ppp2ca knockout in mice spermatogenesis
Abstract
Protein phosphatase 2A (PP2A) is a ubiquitous serine/threonine phosphatase involved in meiosis, mitosis, sperm capacitation, and apoptosis. Abberant activity of PP2A has been associated with a number of diseases. The homolog PPP2CA and PPP2CB can each function as the phosphatase catalytic subunit generally referred to as PP2AC. We generated a Ppp2ca conditional knockout (CKO) in C57BL/6J mice. Exon 2 of Ppp2ca was knocked out in a spatial or temporal-specific manner in primordial germ cells at E12.5. This Ppp2ca-null mutation caused infertility in male C57BL/6J mice. These CKO mice provide a powerful tool to study the mechanisms of Ppp2ca in development and disease.
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Conditional knockout of Ppp2ca in primordial germ cells at E12.5 resulted in male infertility.
“Conditional KO of Ppp2ca in primordial germ cells at E12.5, resulted in male infertility [27].”
From Physiologic functions of PP2A: Lessons from genetically modified mice (2019) · cites this paper as [27]
In that model, testes were smaller, normal tubules containing spermatogonia and primary and secondary spermatocytes were absent, germ cells were disorganized, and elongated spermatozoa were lacking.
“Testes were smaller, normal tubules containing spermatogonia and primary and secondary spermatocytes were absent, germ cells were disorganized, and elongated spermatozoa were lacking all suggestive for a spermatogenesis and meiosis defect.”
From Physiologic functions of PP2A: Lessons from genetically modified mice (2019) · cites this paper as [27]
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References 25 · Cited by 32
- Authors
- Pan, X., Chen, X., Tong, X., Tang, C., & Li, J.
- Affiliations
- Nanjing Medical University (CN)
- Corresponding author
- Li, Jianmin
- Venue
- Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 2015-04-01
- Volume
- 149
- Issue
- 4
- Pages
- 385-391
- ISSN
- 1470-1626 (ISSN-L)
- 1741-7899
- OpenAlex
- W2086584353
- Cited by
- 32
- References
- 25
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 2 · 2023: 1 · 2022: 6 · 2021: 5 · 2020: 2 · 2019: 3 · 2018: 3 · 2017: 3 · 2016: 3
- FWCI
- 3.43
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Reproduction (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Reproductive biology and impacts on aquatic species
- Keywords
- protein phosphatase 2A, PPP2CA, spermatogenesis, conditional knockout, male infertility, primordial germ cells, germ cell development
- MeSH terms
- Animals, Cells, Cultured, Infertility, Male, Male, Meiosis, Mice, Inbred C57BL, RNA, Messenger, Spermatogenesis, Blotting, Western, Mice, Knockout, Integrases, Reverse Transcriptase Polymerase Chain Reaction, Mice, Protein Phosphatase 2, Real-Time Polymerase Chain Reaction
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