Mast cells degranulation affects angiogenesis in the rat uterine cervix during pregnancy.
Open accessAbstract
During pregnancy, it is essential that sufficient nutrients are supplied by the vascular system to support the dramatic modifications of the rat uterine cervix. Angiogenesis refers to the growth of new blood vessels from pre-existing microcirculation and mast cells have been associated with this process. This study examined the modifications of the vascular compartment and the distribution of mast cells on cervical tissue during pregnancy. Using disodium cromoglycate as a mast cell stabilizer, we determined the effects of the mast cell degranulation on cervical angiogenesis. Mast cell distribution and their degranulation status were evaluated by immunohistochemistry. Endothelial cell proliferation was measured by bromodeoxyuridine incorporation. Vascular areas (absolute and relative) and maturation indices were assessed by quantitative immunohistochemistry of von Willebrand factor and alpha-smooth muscle actin respectively. Mast cells were predominantly observed during the first half of pregnancy in the perivascular zones. The values of bromodeoxyuridine incorporation, absolute vascular area and vascular maturation index exhibited a significant increase throughout pregnancy. All animals that received mast cell stabilizer showed more than 40% of non-degranulated mast cells. Treated rats exhibited a decrease in endothelial proliferation and in relative vascular area; in addition, a large proportion of mature blood vessels was observed, suggesting a diminished level of new vessel formation. The effects of the mast cell stabilizer were sustained beyond the end of treatment. This is the first report that brings evidence that mast cell degranulation could be a necessary process to contribute to the normal angiogenesis of the rat cervix during pregnancy. Further investigations are needed to elucidate the possible implications of abnormal vascular development of the uterine cervix on the physiological process of ripening and parturition.
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The number of mast cells in the uterus fluctuates during the menstrual cycle in humans and the estrus cycle in rats and mice, indicating a hormonal influence on their recruitment from the periphery to the uterus.
“The number of MCs in the uterus has been shown to fluctuate during menstrual cycle in human and estrus cycle in rat and mouse indicating a hormonal influence on their recruitment from the periphery to the uterus.”
From Estradiol and Progesterone Regulate the Migration of Mast Cells from the Periphery to the Uterus and Induce Their Maturation and Degranulation (2010) · cites this paper as [25]
The Introduction (page unavailable) describes mast cells in the human uterus and discusses their possible role during the menstrual cycle. The results section on mast cells and vascular parameters (page unavailable) reports changes in cervical mast-cell abundance during rat pregnancy, not during the estrus cycle. Neither passage establishes cycle-related fluctuations in humans, rats and mice or hormone-driven recruitment from the periphery. (LLM, conf=0.78)
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References 57 · Cited by 41
Research works printed but not linked: 2
- Eds E Knobil & JD Neill. New York: Raven Press.
- Sherwood OD 1994 Relaxin. In The Physiology of Reproduction, pp 861 -1009. Eds E Knobil & JD Neill. New York: Raven Press.
- Authors
- Varayoud, J., Ramos, J. G., Bosquiazzo, V. L., Muñoz-de-Toro, M., & Luque, E. H.
- Affiliations
- Universidad Nacional del Litoral (AR)
- Laboratorio de Endocrinología y Tumores Hormonodependientes, School of Biochemistry and Biological Sciences, Universidad Nacional del Litoral, Casilla de Correo 242, (3000) Santa Fe, Argentina
- Corresponding author
- Luque, E H
- Venue
- Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-03-01
- Volume
- 127
- Issue
- 3
- Pages
- 379-387
- ISSN
- 1470-1626 (ISSN-L)
- 1741-7899
- OpenAlex
- W2021211609
- Cited by
- 41
- References
- 57
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 1 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 3 · 2017: 2 · 2016: 1 · 2015: 3 · 2014: 1 · 2013: 2 · 2012: 2
- FWCI
- 0.48
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Reproduction (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Consejo Nacional de Investigaciones Científicas y Técnicas
- Universidad Nacional del Litoral
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Health Sciences › Medicine › Obstetrics and Gynecology › Pregnancy and preeclampsia studies
- Keywords
- mast cells, mast cell degranulation, angiogenesis, uterine cervix, pregnancy, endothelial cell proliferation, vascular maturation, disodium cromoglycate, mast cell stabilizers, cervical ripening, childbirth
- MeSH terms
- Animals, Cell Division, Cervix Uteri, Cromolyn Sodium, Female, Immunohistochemistry, Mast Cells, Pregnancy, Pregnancy, Animal, Random Allocation, Cell Degranulation, Rats, Wistar, Neovascularization, Physiologic, Endothelial Cells, Rats
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