The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
The Broad-Spectrum Blast Resistance Gene Pi9 Encodes a Nucleotide-Binding Site–Leucine-Rich Repeat Protein and Is a Member of a Multigene Family in Rice
Abstract
The broad-spectrum rice blast resistance gene Pi9 was cloned using a map-based cloning strategy. Sequencing of a 76-kb bacterial artificial chromosome (BAC) contig spanning the Pi9 locus led to identification of six tandemly arranged resistance-like genes with a nucleotide-binding site (NBS) and leucine-rich repeats (LRRs) (Nbs1-Pi9–Nbs6-Pi9). Analysis of selected Pi9 deletion mutants and transformation of a 45-kb fragment from the BAC contig into the susceptible rice cultivar TP309 narrowed down Pi9 to the candidate genes Nbs2-Pi9 and Nbs3-Pi9. Disease evaluation of the transgenic lines carrying the individual candidate genes confirmed that Nbs2-Pi9 is the Pi9 gene. Sequence comparison analysis revealed that the six paralogs at the Pi9 locus belong to four classes and gene duplication might be one of the major evolutionary forces contributing to the formation of the NBS–LRR gene cluster. Semiquantitative reverse transcriptase (RT)–PCR analysis showed that Pi9 was constitutively expressed in the Pi9-resistant plants and was not induced by blast infection. The cloned Pi9 gene provides a starting point to elucidate the molecular basis of the broad-spectrum disease resistance and the evolutionary mechanisms of blast resistance gene clusters in rice.
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Roles for Rice Membrane Dynamics and Plasmodesmata during Biotrophic Invasion by the Blast Fungus
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Pan-genome analysis of 33 genetically diverse rice accessions reveals hidden genomic variations
Qin, P., Lu, H., Du, H., Wang, H., Chen, W., Chen, Z., He, Q., Ou, S., Zhang, H., Li, X., et al. (2021) · Cell · doi:10.1016/j.cell.2021.04.046 · Cited by 578
Hasan, N., Choudhary, S., Naaz, N., Sharma, N., & Laskar, R. A. (2021) · Journal of Genetic Engineering and Biotechnology · doi:10.1186/s43141-021-00231-1 · Cited by 513
Miah, G., Rafii, M., Ismail, M., Puteh, A., Rahim, H., Islam, K., & Latif, M. (2013) · International Journal of Molecular Sciences · doi:10.3390/ijms141122499 · Cited by 241
On the move: induced resistance in monocots
Balmer, D., Planchamp, C., & Mauch-Mani, B. (2013) · Journal of Experimental Botany · doi:10.1093/jxb/ers248 · Cited by 77
Metadata
- Authors
- Qu, S., Liu, G., Zhou, B., Bellizzi, M., Zeng, L., Dai, L., Han, B., & Wang, G.-L.
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-03-01
- Volume
- 172
- Issue
- 3
- Pages
- 1901-1914
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- OpenAlex
- W2156611167
- Cited by
- 627
- References
- 4
- FWCI
- 21.03
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: The Broad-Spectrum Blast Resistance Gene Pi9 Encodes a Nucleotide-Binding Site–Leucine-Rich Repeat Protein and Is a Member of a Multigene Family in Rice. https://app.cochat.ai/papers/10.1534/genetics.105.044891
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- Qu, S., Liu, G., Zhou, B., Bellizzi, M., Zeng, L., Dai, L., Han, B., & Wang, G.-L. (2006). The Broad-Spectrum Blast Resistance Gene Pi9 Encodes a Nucleotide-Binding Site–Leucine-Rich Repeat Protein and Is a Member of a Multigene Family in Rice. Genetics. https://doi.org/10.1534/genetics.105.044891
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