The Genetic Basis of Zinc Tolerance in the Metallophyte Arabidopsis halleri ssp. halleri (Brassicaceae): An Analysis of Quantitative Trait Loci
Open accessAbstract
The species Arabidopsis halleri, an emerging model for the study of heavy metal tolerance and accumulation in plants, has evolved a high level of constitutive zinc tolerance. Mapping of quantitative trait loci (QTL) was used to investigate the genetic architecture of zinc tolerance in this species. A first-generation backcross progeny of A. halleri ssp. halleri from a highly contaminated industrial site and its nontolerant relative A. lyrata ssp. petraea was produced and used for QTL mapping of zinc tolerance. A genetic map covering most of the A. halleri genome was constructed using 85 markers. Among these markers, 65 were anchored in A. thaliana and revealed high synteny with other Arabidopsis genomes. Three QTL of comparable magnitude on three different linkage groups were identified. At all QTL positions zinc tolerance was enhanced by A. halleri alleles, indicating directional selection for higher zinc tolerance in this species. The two-LOD support intervals associated with these QTL cover 24, 4, and 13 cM. The importance of each of these three regions is emphasized by their colocalization with HMA4, MTP1-A, and MTP1-B, respectively, three genes well known to be involved in metal homeostasis and tolerance in plants.
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At least three QTLs possibly belonging to hypertolerance of Cd and Zn have been mapped.
“At least tree Quantitative trait locus (QTLs) possibly belonging to hypertolerance of Cd and Zn have been mapped (Courbot et al., 2007; Willems et al., 2007).”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Willems et al., 2007
The Results identify three mapped QTL for Zn tolerance. The Discussion reports that one overlaps a major Cd-tolerance QTL, but does not establish that all three confer Cd tolerance. It also says it is unclear whether all three contribute to enhanced tolerance rather than constitutive tolerance. (LLM, conf=0.88)
Experimental — accuracy not yet measuredAn overlapping QTL identified in the AhHMA4 gene in Arabidopsis halleri is also screened.
“Moreover, an overlapping QTL identified in the AhHMA4 (Heavy Metal ATPase 4) gene in Arabidopsis halleri, a hyperaccumulator of Cd and Zn, is also screened (Courbot et al., 2007; Willems et al., 2007) ( Figure 1 ).”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Willems et al., 2007
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References 74 · Cited by 182
Research works printed but not linked: 2
- Beavis, W. D., 1994 The power and deceit of QTL experiments: les- sons from comparative QTL studies, pp. 250-266 in Proceedings of the 49th Annual Corn and Sorghum Industry Research Conference, edited by A. S. Trade. ASTA, Chicago.
- Saumitou-Laprade, P., Y. Piquot, O. Raspe ´, J. Bernard and K. Vrieling, 1999 Plant DNA fingerprinting and profiling, pp. 17-38 in DNA Profiling and DNA Fingerprinting, edited by J. T. Epplen and T. Lubjuhn. Birkhauser, Basel, Switzerland.
- Authors
- Willems, G., Dräger, D. B., Courbot, M., Godé, C., Verbruggen, N., & Saumitou-Laprade, P.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Max Planck Institute of Molecular Plant Physiology, D-14424 Potsdam, Germany and
- Laboratoire de Physiologie et de Génétique Moléculaire des Plantes, Université Libre de Bruxelles, B-1050 Brussels, Belgium
- Université Libre de Bruxelles (BE)
- Laboratoire de Génétique et Evolution des Populations Végétales, UMR CNRS 8016, FR CNRS 1818, Université des Sciences et Technologies de Lille–Lille1, F-59655 Villeneuve d'Ascq Cedex, France
- ORCID
- Verbruggen, Nathalie
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-05-01
- Volume
- 176
- Issue
- 1
- Pages
- 659-674
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- PMC
- PMC1893047
- OpenAlex
- W2134072683
- Cited by
- 182
- References
- 74
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 3 · 2020: 18 · 2019: 8 · 2018: 9 · 2017: 12 · 2016: 14 · 2015: 13 · 2014: 7 · 2013: 12 · 2012: 17
- FWCI
- 6.96
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Genetics (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Dépôt institutionnel de l'Université libre de Bruxelles (Université Libre de Bruxelles) (submittedVersion)
- HAL (submittedVersion)
- Funders
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Mapping and Diversity in Plants and Animals
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
- Physical Sciences › Environmental Science › Pollution › Heavy metals in environment
- Keywords
- zinc tolerance, Arabidopsis halleri, metallophytes, quantitative trait loci, QTL mapping, heavy metal tolerance, metal homeostasis, HMA4, Arabidopsis lyrata, genetic linkage map, synteny, directional selection, genetic architecture
- MeSH terms
- Adaptation, Physiological, Chromosome Mapping, Clone Cells, Epistasis, Genetic, Genetic Markers, Lod Score, Zinc, Arabidopsis, Chromosome Segregation, Quantitative Trait Loci
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