The Hog1 Mitogen-Activated Protein Kinase Mediates a Hypoxic Response in Saccharomyces cerevisiae
Open accessAbstract
We have studied hypoxic induction of transcription by studying the seripauperin (PAU) genes of Saccharomyces cerevisiae. Previous studies showed that PAU induction requires the depletion of heme and is dependent upon the transcription factor Upc2. We have now identified additional factors required for PAU induction during hypoxia, including Hog1, a mitogen-activated protein kinase (MAPK) whose signaling pathway originates at the membrane. Our results have led to a model in which heme and ergosterol depletion alters membrane fluidity, thereby activating Hog1 for hypoxic induction. Hypoxic activation of Hog1 is distinct from its previously characterized response to osmotic stress, as the two conditions cause different transcriptional consequences. Furthermore, Hog1-dependent hypoxic activation is independent of the S. cerevisiae general stress response. In addition to Hog1, specific components of the SAGA coactivator complex, including Spt20 and Sgf73, are also required for PAU induction. Interestingly, the mammalian ortholog of Spt20, p38IP, has been previously shown to interact with the mammalian ortholog of Hog1, p38. Taken together, our results have uncovered a previously unknown hypoxic-response pathway that may be conserved throughout eukaryotes.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In addition to Upc2, the MAPK Hog1 and specific components of the SAGA co-activator complex also contribute to the induction of PAU genes in response to oxygen limitation.
“In addition to Upc2, the mitogen-activated protein kinase (MAPK) Hog1 and specific components of the SAGA co-activator complex also contribute to the induction of PAU genes in response to oxygen limitation [96].”
From Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae (2020) · cites this paper as [96]
Several genome-wide expression studies under low oxygen conditions have reflected that the pattern of expression differs among ERG genes, although some general trends can be extracted.
“Several genome-wide expression studies based on microarray and RNA-Seq analyses under low oxygen conditions have reflected that the pattern of expression differs among ERG genes, although some general trends can be extracted [94,96,98,100,101,102,103].”
From Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae (2020) · cites this paper as [96]
Conflicting results have been observed for the effect of Hap1 on the expression of ERG genes during anaerobic conditions.
“However, conflicting results have been observed for the effect of Hap1 on the expression of ERG genes during anaerobic conditions [75,96,99,100].”
From Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae (2020) · cites this paper as [96]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 124 · Cited by 68
- Authors
- Hickman, M. J., Spatt, D., & Winston, F.
- Affiliations
- Harvard University (US)
- Corresponding author
- Winston, Fred
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-06-01
- Volume
- 188
- Issue
- 2
- Pages
- 325-338
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- PMC
- PMC3122313
- OpenAlex
- W2063436167
- Cited by
- 68
- References
- 124
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 5 · 2023: 2 · 2022: 1 · 2021: 7 · 2020: 6 · 2019: 3 · 2018: 3 · 2017: 9 · 2016: 3 · 2015: 6 · 2014: 4 · 2013: 6 · 2012: 6
- FWCI
- 2.08
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Genetics (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Princeton University
- National Institutes of Health (GM046406)
- National Institute of General Medical Sciences (F32GM071102, R01GM045720, R37GM046406, R01GM046406, GM046406, F32GM71102, P50GM071508, GM 45720)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- Hog1, mitogen-activated protein kinase, hypoxia response, ergosterol depletion, membrane fluidity, p38 MAPK, osmotic stress, Saccharomyces cerevisiae, transcriptional regulation
- MeSH terms
- p300-CBP-Associated Factor, Anaerobiosis, Ergosterol, Heme, Membrane Fluidity, Membrane Proteins, Mutation, Saccharomyces cerevisiae, Transcription Factors, Blotting, Northern, Blotting, Western, Trans-Activators, Gene Expression Regulation, Fungal, Gene Expression Regulation, Developmental, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Mitogen-Activated Protein Kinases, Saccharomyces cerevisiae Proteins, Histone Acetyltransferases
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
