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Cited by 77Fungal and yeast genetics researchDNA Repair MechanismsMitochondrial Function and Pathology

A Genome-Wide Map of Mitochondrial DNA Recombination in Yeast

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Fritsch, Emilie S, Chabbert, Christophe D, Klaus, Bernd, Steinmetz, Lars M·2014·Genetics
DOI 10.1534/genetics.114.166637PMC PMC4196626
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Abstract

In eukaryotic cells, the production of cellular energy requires close interplay between nuclear and mitochondrial genomes. The mitochondrial genome is essential in that it encodes several genes involved in oxidative phosphorylation. Each cell contains several mitochondrial genome copies and mitochondrial DNA recombination is a widespread process occurring in plants, fungi, protists, and invertebrates. Saccharomyces cerevisiae has proved to be an excellent model to dissect mitochondrial biology. Several studies have focused on DNA recombination in this organelle, yet mostly relied on reporter genes or artificial systems. However, no complete mitochondrial recombination map has been released for any eukaryote so far. In the present work, we sequenced pools of diploids originating from a cross between two different S. cerevisiae strains to detect recombination events. This strategy allowed us to generate the first genome-wide map of recombination for yeast mitochondrial DNA. We demonstrated that recombination events are enriched in specific hotspots preferentially localized in non-protein-coding regions. Additionally, comparison of the recombination profiles of two different crosses showed that the genetic background affects hotspot localization and recombination rates. Finally, to gain insights into the mechanisms involved in mitochondrial recombination, we assessed the impact of individual depletion of four genes previously associated with this process. Deletion of NTG1 and MGT1 did not substantially influence the recombination landscape, alluding to the potential presence of additional regulatory factors. Our findings also revealed the loss of large mitochondrial DNA regions in the absence of MHR1, suggesting a pivotal role for Mhr1 in mitochondrial genome maintenance during mating. This study provides a comprehensive overview of mitochondrial DNA recombination in yeast and thus paves the way for future mechanistic studies of mitochondrial recombination and genome maintenance.

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  • The identical mitochondrial genomes of the Swiss and clinical C. subhashii isolates are in contrast to studies reporting considerable intra-species variation in size, intron content, and recombination in fungal mitochondrial genomes.

    “the identical mitochondrial genomes are in contrast to studies reporting considerable intra-species variation in size, intron content, and recombination in fungal mitochondrial genomes [104–106].”

    From Competition assays and physiological experiments of soil and phyllosphere yeasts identify Candida subhashii as a novel antagonist of filamentous fungi (2017) · cites this paper as [105]

    The Results section, “Intraspecies variability influences recombination hotspot profiles” (page unavailable), reports differences in mitochondrial recombination rates and hotspots between S. cerevisiae crosses. The Discussion section, “The genetic background influences mitochondrial recombination” (page unavailable), notes an additional COX1 intron in one strain. These passages support variation in recombination and intron content, but do not establish intra-species variation in mitochondrial genome size. (LLM, conf=0.86)

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Cited by

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Authors
Fritsch, E. S., Chabbert, C. D., Klaus, B., & Steinmetz, L. M.
Affiliations
  • European Molecular Biology Laboratory (DE)
  • Stanford Medicine (US)
Corresponding author
Steinmetz, Lars M
ORCID
Venue
Genetics
Publisher
Oxford University Press (OUP)
Published
2014-07-31
Volume
198
Issue
2
Pages
755-771
ISSN
  • 0016-6731 (ISSN-L)
  • 1943-2631
DOI
10.1534/genetics.114.166637
PMC
PMC4196626
OpenAlex
W2021051845
Cited by
77
References
4
Citations by year
2026: 2 · 2025: 3 · 2024: 4 · 2023: 6 · 2022: 9 · 2021: 7 · 2020: 8 · 2019: 8 · 2018: 5 · 2017: 12 · 2016: 4 · 2015: 9
FWCI
3.42
Type
journal article
Language
en
Open access
Licence
academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
Locations
  • Genetics (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
Keywords
mitochondrial recombination, Saccharomyces cerevisiae, recombination hotspots, mitochondrial DNA maintenance, genetic background effects, mitochondrial DNA loss, mating
MeSH terms
Chromosome Mapping, DNA, Mitochondrial, Recombination, Genetic, Saccharomyces cerevisiae, Genome, Fungal, Sequence Analysis, DNA, Mutation Rate

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