Nano silver treatment is effective in reducing bacterial contaminations of Araucaria excelsa R. Br. var. glauca explants
Abstract
The downside of plant tissue culture techniques is an unwanted microbial contamination. Elimination of contaminants is the first step of any successful investigation on plant tissue culture. Preliminary experiments on Araucaria excelsa R. Br. var. glauca (Norfolk-Island pine) (syn.: A. heterophylla) showed that most common decontaminants could not successfully eliminate the contamination. Therefore, nano silver (NS) colloids were evaluated for controlling contamination. Treatments were included soaking the explants in NS solution or adding NS to the culture medium. Explants were cultured on MS medium supplemented with appropriate growth regulators for their establishment. Results showed that surface sterilization followed by treatment with 200 mg l-1 of NS with soaking time of 180 min reduced the bacterial contamination from 61.5% to 11.3% and adding 400 mg l-1 NS to the medium reduced the bacterial contamination from 81.25% to 18.75%. Nano silver could be applied without adverse effects on plant growth and development. This is the first report on in vitro establishment of A. excelsa R. Br. using NS to reduce bacterial infections.
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Explants of Araucaria excelsa, Cynodon dactylon, Gerbera jamesonii, olive, Valeriana officinalis, and Vitis vinifera obtained from greenhouse- or field-grown plants were disinfected with ethanol or commercial bleach, and then silver nanoparticles reduced or eliminated the contaminants.
“The explants of Araucaria excelsa, Cynodon dactylon, Gerbera jamesonii, olive, Valeriana officinalis, and Vitis vinifera obtained from greenhouse- or field-grown plants were disinfected with ethanol or commercial bleach, and then silver nanoparticles (AgNPs) eventually reduced or eliminated the contaminants [11,12,13,14,15].”
From A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture (2021) · cites this paper as [12]
Inclusion of Ag NPs, TiO2 NPs, Zn NPs or ZnO NPs in the culture medium reduces internal and external contamination in Araucaria excelsa, Bacopa monnieri, banana, barley, G × N15 rootstocks, olive, potato, Rosa hybrida, Vanilla planifolia, and tobacco.
“Inclusion of Ag NPs, TiO2 NPs, Zn NPs or ZnO NPs in the culture medium is reported to reduce internal and external contamination in Araucaria excelsa, Bacopa monnieri, banana, barley, G × N15 (hybrid of almond × peach) rootstocks, olive, potato, Rosa hybrida, Vanilla planifolia, and tobacco [12,16,18,19,20,21,22,23,24,25,26,27].”
From A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture (2021) · cites this paper as [12]
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A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture
Sivanesan, I., Muthu, M., Gopal, J., Tasneem, S., Kim, D.-H., & Oh, J.-W. (2021) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph18052282 · Cited by 27 · see citing works
- Authors
- Sarmast, M., Salehi, H., & Khosh-Khui, M.
- Affiliations
- Shiraz University (IR)
- Venue
- Acta Biologica Hungarica
- Published
- 2011-12
- Volume
- 62
- Issue
- 4
- Pages
- 477-484
- ISSN
- 0001-5288 (ISSN-L)
- 0236-5383
- 1588-256X
- OpenAlex
- W1987249708
- Cited by
- 60
- References
- 0
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 6 · 2023: 3 · 2022: 7 · 2021: 8 · 2020: 6 · 2019: 7 · 2018: 2 · 2017: 4 · 2016: 2 · 2014: 5 · 2013: 2 · 2012: 2
- FWCI
- 1.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Acta Biologica Hungarica (publishedVersion)
- PubMed (publishedVersion)
- Repository of the Academy's Library (Library of the Hungarian Academy of Sciences) (acceptedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Disease Management Techniques
- Keywords
- silver nanoparticles, bacterial contamination, microbial decontamination, culture medium supplementation, surface sterilization, in vitro establishment, plant tissue culture
- MeSH terms
- Anti-Bacterial Agents, Colloids, Culture Media, Silver, Tissue Culture Techniques, Metal Nanoparticles, Tracheophyta
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