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Cited by 35Traditional and Medicinal Uses of AnnonaceaePhytochemical Studies and BioactivitiesEssential Oils and Antimicrobial Activity

Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts

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PINTO, NÍCOLAS C.C., SILVA, JUCÉLIA B., MENEGATI, LAURA M., GUEDES, MARIA CLARA M.R., MARQUES, LUCAS B., SILVA, THIAGO P. DA, MELO, ROSSANA C.N. DE, SOUZA-FAGUNDES, ELAINE M. DE +3 more·2017·Anais da Academia Brasileira de Ciências
DOI 10.1590/0001-3765201720150702
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Abstract

This study aimed to further investigate the cytotoxicity against tumor cell lines and several bacterial strains of Annona squamosa and its mode of action. Methanol extracts of A. squamosa leaves (ASL) and seeds (ASS) were used. ASL showed significant antibacterial activity against S. aureus, K. pneumoniae and E. faecalis with MIC values of 78, 78 and 39 µg/mL respectively. Moreover, ASL exhibited significant biofilm disruption, rapid time dependent kinetics of bacterial killing, increased membrane permeability and significantly reduced the cell numbers and viability. Regarding the cytotoxicity against tumor cell lines, ASS was more active against Jurkat and MCF-7 cells, with CI50 1.1 and 2.1 µg/mL, respectively. ASL showed promising activity against Jurkat and HL60, with CI50 4.2 and 6.4 µg/mL, respectively. Both extracts showed lower activity against VERO cells and reduced the clonogenic survival at higher concentrations (IC90) to MCF-7 and HCT-116 lineages. The alkaloids anonaine, asimilobine, corypalmine, liriodenine nornuciferine and reticuline were identified in extracts by UPLC-ESI-MS/MS analysis. This study reinforced that A. squamosa presents a remarkable phytomedicinal potential and revealed that its antimicrobial mechanism of action is related to bacterial membrane destabilization.

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  • Human red blood cell membrane stabilisation is indicative of anti-inflammatory activity.

    “Human red blood cells (HRBCs) were used to evaluate membrane stabilisation potential, which is indicative of anti-inflammatory activity (Nguyen et al., 2026;Pinto et al., 2017;Utterström et al., 2022;Zhang et al., 2026).”

    From PHYTOCHEMICAL CHARACTERIZATION AND EVALUATION OF ANTIOXIDANT, ANTIMICROBIAL, ANTI-INFLAMMATORY, AND CYTOTOXIC ACTIVITIES OF SEQUENTIAL EXTRACTS FROM RIPE BANANA (MUSA PARADISIACA L.) PEEL (2026) · cites this paper as Pinto et al., 2017

    The BACTERIA VIABILITY and ANTIMICROBIAL ACTIVITY sections discuss bacterial membrane integrity, not human red blood cell membrane stabilisation. Searches for human red blood cells, erythrocytes, haemolysis, and anti-inflammatory activity found no passage addressing the claimed link. Page numbers were not available in our copy. (LLM, conf=0.75)

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Authors
PINTO, N. C. C., SILVA, J. B., MENEGATI, L. M., GUEDES, M. C. M. R., MARQUES, L. B., SILVA, T. P. D., MELO, R. C. N. D., SOUZA-FAGUNDES, E. M. D., SALVADOR, M. J., SCIO, E., & FABRI, R. L.
Affiliations
  • Universidade Federal de Juiz de Fora, Brazil
  • Universidade Federal de Juiz de Fora (BR)
  • Universidade Federal de Minas Gerais (BR)
  • Universidade Estadual de Campinas (UNICAMP) (BR)
ORCID
Venue
Anais da Academia Brasileira de Ciências
Publisher
FapUNIFESP (SciELO)
Published
2017-08-14
Volume
89
Issue
3 suppl
Pages
2053-2073
ISSN
  • 0001-3765 (ISSN-L)
  • 1678-2690
DOI
10.1590/0001-3765201720150702
OpenAlex
W2742198224
Cited by
35
References
0
Citations by year
2026: 2 · 2025: 2 · 2024: 4 · 2023: 3 · 2022: 7 · 2021: 7 · 2020: 7 · 2019: 2 · 2018: 1
FWCI
1.39
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Anais da Academia Brasileira de Ciências (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
  • Americanae (AECID Library) (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Redalyc (Universidad Autónoma del Estado de México) (submittedVersion)
Funders
  • Fundação de Amparo à Pesquisa do Estado de São Paulo
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • Fundação de Amparo à Pesquisa do Estado de Minas Gerais
  • Universidade Federal de Juiz de Fora
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Traditional and Medicinal Uses of Annonaceae
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phytochemical Studies and Bioactivities
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
Keywords
Annona squamosa, custard apple, antibacterial activity, cytotoxicity, membrane permeability, biofilm eradication, cancer cell lines, Jurkat cells, MCF-7 cells, HL-60 cells, alkaloids, anonaine, asimilobine, liriodenine, nornuciferine, reticuline, herbal medicine
MeSH terms
Animals, Anti-Bacterial Agents, Cell Membrane, Chlorocebus aethiops, Humans, Klebsiella pneumoniae, Microbial Sensitivity Tests, Plant Extracts, Staphylococcus aureus, Enterococcus faecalis, Annona, Cell Line, Tumor

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