Sustainable bioformulation of Trichoderma asperellum, Pseudomonas fluorescens, and Bacillus subtilis enhances agricultural productivity in different Brazilian states

Abstract

ABSTRACT The use of plant growth-promoting microorganisms (PGPM) is a promising strategy to enhance crop productivity while improving soil functionality. This study evaluated the efficacy of fungal-bacterial consortium of Trichoderma asperellum, Pseudomonas fluorescens, and Bacillus subtilis, for promoting the growth of soybean and maize cultivated under distinct edaphoclimatic conditions across Brazil. Field trials were conducted in five locations within Rio Grande do Sul, Santa Catarina, São Paulo, and Minas Gerais. Treatments consisted of a fungal-bacterial consortium (200 g ha-¹, in-furrow at planting) combined with 50% or 100% of the recommended nitrogen rate. Shoot dry biomass, foliar nitrogen (N) and phosphorus (P) concentrations, grain yield, and soil microbial activity determined by fluorescein diacetate hydrolysis were assessed. The fungal-bacterial consortium significantly improved all variables in both crops. In soybean, shoot biomass increased by 10.7-13.4% and grain yield by 9.2-9.9%, while foliar N and P rose by 10.2-12.4%, and soil enzymatic activity increased up to 11.0%. In maize, biomass increased by 10.4-11.8% and grain yield by 13.1-13.9%, with foliar N and P increasing by 9.5-14.1% and soil enzymatic activity by up to 12.5%. Notably, positive responses were maintained under 50% nitrogen fertilization. These findings demonstrate that fungal-bacterial consortium enhances nutrient acquisition and soil microbial activity, improving crop performance under variable environmental conditions and reduced N input, supporting its potential as a biological tool for sustainable nutrient management in soybean and maize systems.