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Cited by 28Heavy metals in environmentHeavy Metal Pollution RemediationAdsorption and biosorption for pollutant removal

Tolerance to and Accumulation of Cadmium, Copper, and Zinc by Cupriavidus necator

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Vicentin, Rayssa Pereira, Santos, Jessé Valentim dos, Labory, Cláudia Regina Gontijo, Costa, Amanda Monique da, Moreira, Fatima Maria de Souza, Alves, Eduardo·2018·Revista Brasileira de Ciência do Solo
DOI 10.1590/18069657rbcs20170080
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Abstract

Preliminary results of in vitro experiments with multicontaminated soils and solid media indicated that nodulating diazotrophic bacteria of the genus Cupriavidus are promising for the remediation of contaminated environments due to their symbiosis with legumes and metal tolerance. Thus, strains of Cupriavidus spp. (LMG 19424T, UFLA 01-659, UFLA 01-663, and UFLA 02-71) were tested for their ability to tolerate and bioaccumulate cadmium (Cd), copper (Cu), and zinc (Zn) in Luria-Bertani broth. Changes in the growth pattern of Cupriavidus strains in the presence or absence of heavy metals were analyzed by scanning electron microscopy and metal allocation by transmission electron microscopy, to clarify the mechanisms of bioremediation. Highest tolerance was detected for strain UFLA 01-659 (minimum inhibitory concentration of 5, 4.95, and 14.66 mmol L−1 of Cd, Cu, and Zn, respectively). Among the removal rates of the metals tested (9.0, 4.6, and 3.2 mg L−1 of Cd, Cu, and Zn, respectively), the bacterial activity was clearly highest for Cd. The efficiency of strain UFLA 01-659 in removing the heavy metals is associated with its high biomass production and/or higher contents of heavy metals adsorbed and absorbed in the biomass. In response to the presence of heavy metals in the liquid culture medium, the bacteria produced exopolysaccharides and small and aggregated cells. However, these responses varied according to the strains and heavy metals. Regarding allocation, all heavy metals were adsorbed on the cell wall and membrane, whereas complexation was observed intracellularly and only for Cu and Zn. These results indicate the possibility of using C. necator UFLA 01-659 for remediation in areas with very high Cd, Cu, and Zn contents.

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Cited by

Sorted by citations

Authors
Vicentin, R. P., Santos, J. V. D., Labory, C. R. G., Costa, A. M. D., Moreira, F. M. D. S., & Alves, E.
Affiliations
  • Universidade Federal de Lavras (BR)
  • Universidade Federal de Lavras, Brasil
ORCID
Venue
Revista Brasileira de Ciência do Solo
Publisher
FapUNIFESP (SciELO)
Published
2018-03-08
Volume
42
Issue
0
ISSN
  • 0100-0683 (ISSN-L)
  • 1806-9657
DOI
10.1590/18069657rbcs20170080
OpenAlex
W2793765975
Cited by
28
References
0
Citations by year
2026: 2 · 2025: 3 · 2024: 3 · 2023: 5 · 2022: 5 · 2021: 6 · 2020: 3 · 2019: 1
FWCI
0.94
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Revista Brasileira de Ciência do Solo (publishedVersion, cc-by)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, cc-by)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
  • Americanae (AECID Library) (submittedVersion, cc-by)
  • Americanae (AECID Library) (submittedVersion, cc-by-nc)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by)
Funders
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • Universidade Federal de Minas Gerais
Topics
  • Physical Sciences › Environmental Science › Pollution › Heavy metals in environment
  • Physical Sciences › Environmental Science › Water Science and Technology › Heavy Metal Pollution Remediation
  • Physical Sciences › Environmental Science › Water Science and Technology › Adsorption and biosorption for pollutant removal
Keywords
Cupriavidus necator, heavy metal tolerance, cadmium, copper, zinc, metal bioaccumulation, bioremediation, exopolysaccharide production, minimum inhibitory concentration, metal-contaminated soil

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