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Cited by 141Essential Oils and Antimicrobial ActivityPhytochemical compounds biological activitiesAntifungal resistance and susceptibility

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Antifungal activity of different neem leaf extracts and the nimonol against some important human pathogens

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Mahmoud, D.A, Hassanein, N.M, Youssef, K.A, Abou Zeid, M.A·2011·Brazilian Journal of Microbiology
DOI 10.1590/s1517-83822011000300021PMC PMC3768785
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Abstract

This study was conducted to evaluate the effect of aqueous, ethanolic and ethyl acetate extracts from neem leaves on growth of some human pathogens (Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus terreus, Candida albicans and Microsporum gypseum) in vitro. Different concentrations (5, 10, 15 and 20%) prepared from these extracts inhibited the growth of the test pathogens and the effect gradually increased with concentration. The 20% ethyl acetate extract gave the strongest inhibition compared with the activity obtained by the same concentration of the other extracts. High Performance Liquid Chromatography (HPLC) analysis of ethyl acetate extract showed the presence of a main component (nimonol) which was purified and chemically confirmed by Nuclear Magnetic Resonance (NMR) spectroscopic analysis. The 20% ethyl acetate extract lost a part of its antifungal effect after pooling out the nimonol and this loss in activity was variable on test pathogens. The purified nimonol as a separate compound did not show any antifungal activity when assayed against all the six fungal pathogens.

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Crossref · 10.1590/s1517-83822011000300021

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Figure 1 (A): Effect of different concentrations (5%) of aqueous (Aq.), ethanolic (Et.) and ethylacetate (EtoAc) leaf extracts of neem on growth of pathogenic fungi on solid media. (B): Effect of different concentrations (10%) of aqueous (Aq.), ethanolic (Et.) and ethylacetate (EtoAc) leaf extracts of neem on growth of pathogenic fungi on solid media. (C): Effect of different concentrations (15%) of aqueous (Aq.), ethanolic (Et.) and ethylacetate (EtoAc) leaf extracts of neem on growth of pathogenic fungi on solid media. (D): Effect of different concentrations (20%) of aqueous (Aq.), ethanolic (Et.) and ethylacetate (EtoAc) leaf extracts of neem on growth of pathogenic fungi on solid media.
Figure 1 (A): Effect of different concentrations (5%) of aqueous (Aq.), ethanolic (Et.) and ethylacetate (EtoAc) leaf extracts of neem on growth of pathogenic fungi on solid media. (B): Effect of different concentrations (10%) of aqueous (Aq.), ethanolic (Et.) and ethylacetate (EtoAc) leaf extracts of neem on growth of pathogenic fungi on solid media. (C): Effect of different concentrations (15%) of aqueous (Aq.), ethanolic (Et.) and ethylacetate (EtoAc) leaf extracts of neem on growth of pathogenic fungi on solid media. (D): Effect of different concentrations (20%) of aqueous (Aq.), ethanolic (Et.) and ethylacetate (EtoAc) leaf extracts of neem on growth of pathogenic fungi on solid media.CC BY-NC · PubMed Central
Figure 3 High performance liquid chromatographic pattern of neem leaf ethylacetate extract.
Figure 3 High performance liquid chromatographic pattern of neem leaf ethylacetate extract.CC BY-NC · PubMed Central
Figure 2 Percentage of inhibition of different ethylacetate extracts (Aand B) and pure nimonol on the growth of pathogenic fungi.
Figure 2 Percentage of inhibition of different ethylacetate extracts (Aand B) and pure nimonol on the growth of pathogenic fungi.CC BY-NC · PubMed Central

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Citations

Cites

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Research works printed but not linked: 15

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Metadata

Authors
Mahmoud, D. A., Hassanein, N. M., Youssef, K. A., & Abou Zeid, M. A.
Affiliations
Ain Shams University (EG)
ORCID
Hassanein, N.M
Venue
Brazilian Journal of Microbiology
Publisher
FapUNIFESP (SciELO)
Published
2011-09
Volume
42
Issue
3
Pages
1007-1016
ISSN
1517-8382 (ISSN-L)
DOI
10.1590/s1517-83822011000300021
PMC
PMC3768785
OpenAlex
W2065878977
Cited by
141
References
1
Citations by year
2026: 7 · 2025: 14 · 2024: 10 · 2023: 17 · 2022: 25 · 2021: 11 · 2020: 10 · 2019: 12 · 2018: 9 · 2017: 9 · 2016: 5 · 2015: 6 · 2014: 5 · 2013: 1
FWCI
2.81
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • Brazilian Journal of Microbiology (publishedVersion, cc-by-nc)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
  • Americanae (AECID Library) (submittedVersion, cc-by-nc)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Drug Discovery › Phytochemical compounds biological activities
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
Keywords
neem leaf extract, antifungal activity, human fungal pathogens, Aspergillus, Candida albicans, Microsporum gypseum, ethyl acetate extract, aqueous and ethanolic extracts, structure-activity relationship

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