Fractionated Radiation Alters Oncomir and Tumor Suppressor miRNAs in Human Prostate Cancer Cells
Open accessAbstract
We have previously demonstrated that prostate carcinoma cells exposed to fractionated radiation differentially expressed more genes compared to single-dose radiation. To understand the role of miRNA in regulation of radiation-induced gene expression, we analyzed miRNA expression in LNCaP, PC3 and DU145 prostate cancer cells treated with single-dose radiation and fractionated radiation by microarray. Selected miRNAs were studied in RWPE-1 normal prostate epithelial cells by RT-PCR. Fractionated radiation significantly altered more miRNAs as compared to single-dose radiation. Downregulation of oncomiR-17-92 cluster was observed only in the p53 positive LNCaP and RWPE-1 cells treated with single-dose radiation and fractionated radiation. Comparison of miRNA and mRNA data by IPA target filter analysis revealed an inverse correlation between miR-17-92 cluster and several targets including TP53INP1 in p53 signaling pathway. The base level expressions of these miRNAs were significantly different among the cell lines and did not predict the radiation outcome. Tumor suppressor miR-34a and let-7 miRNAs were upregulated by fractionated radiation in radiosensitive LNCaP (p53 positive) and PC3 (p53-null) cells indicating that radiation-induced miRNA expression may not be regulated by p53 alone. Our data support the potential for using fractionated radiation to induce molecular targets and radiation-induced miRNAs may have a significant role in predicting radiosensitivity.
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The effect of absorbed dose on gene expression has only recently been investigated.
“Only recently, the effect of absorbed dose on gene expression has been investigated ( 110 ).”
From Normal-Tissue Tolerance to Radiopharmaceutical Therapies, the Knowns and the Unknowns (2021) · cites this paper as [110]
The Introduction (page not provided) says that recent work compared gene expression after single-dose and fractionated radiation, and the present study examines miRNA expression under different dose regimens. It also says earlier studies evaluated miRNA expression after different radiation doses, so it does not support the broader assertion that the effect of absorbed dose on gene expression was investigated only recently. (LLM, conf=0.84)
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References 60 · Cited by 75
- Authors
- John-Aryankalayil, M., Palayoor, S. T., Makinde, A. Y., Cerna, D., Simone, C. B., Falduto, M. T., Magnuson, S. R., & Coleman, C. N.
- Affiliations
- Radiation Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland; and
- National Institutes of Health (US)
- GenUs BioSystems (United States) (US)
- Venue
- Radiation Research
- Publisher
- Radiation Research Society
- Published
- 2012-09-01
- Volume
- 178
- Issue
- 3
- Pages
- 105
- ISSN
- 0033-7587 (ISSN-L)
- PMC
- PMC4156966
- OpenAlex
- W2171974079
- Cited by
- 75
- References
- 60
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 6 · 2023: 2 · 2022: 2 · 2021: 5 · 2020: 8 · 2018: 8 · 2017: 12 · 2016: 8 · 2015: 1 · 2014: 11 · 2013: 8 · 2012: 2
- FWCI
- 2.34
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Radiation Research (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- BioOne Complete (BioOne) (submittedVersion)
- Funders
- National Institutes of Health
- National Cancer Institute (ZIABC010670, Z01BC010670)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Keywords
- fractionated irradiation, microRNA expression, prostate cancer, miR-34a, let-7 microRNA, tumor suppressor microRNA, p53 pathway, TP53INP1, radiosensitivity, LNCaP cells, PC-3 cells, RWPE-1 cells, radiation-induced gene expression
- MeSH terms
- Epithelial Cells, Hot Temperature, Humans, Immunity, Innate, Male, Prostatic Neoplasms, Radiation Tolerance, Reproducibility of Results, Dose Fractionation, Radiation, MicroRNAs, Cell Line, Tumor, Transcriptome
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