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Cited by 12Mesenchymal stem cell researchLiver Disease and TransplantationLiver physiology and pathology

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Effect of undifferentiated versus hepatogenic partially differentiated mesenchymal stem cells on hepatic and cognitive functions in liver cirrhosis

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Elberry, Dalia Azmy, Amin, Shaimaa Nasr, Esmail, Reham Shehab El Nemr, Rashed, Laila Ahmed, Gamal, Maha Mohamed·2016·IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund
DOI 10.17179/excli2016-645PMC PMC5318675
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Abstract

Liver cirrhosis is the outcome of chronic liver injury. The current study aimed to investigate the therapeutic effect of undifferentiated mesenchymal stem cells versus in vitro partially differentiated mesenchymal stem cells on liver cirrhosis and hepatic encephalopathy. 50 adult male albino rats constituted the animal model and were divided into the following groups: control, thioacetamide, undifferentiated mesenchymal stem cells and hepatocyte growth factor-differentiated mesenchymal stem cells groups. Cognitive assessment was achieved by open field test and Y-maze task. We measured serum alanine aminotransferase, albumin and transforming growth factor-beta1, gene expression of α-smooth muscle actin, matrix metalloprotein-2, its tissue inhibitor and apoptotic markers: Bax and Bcl2, brain glial fibrillary acidic protein, synaptophysin, and dopaminergic receptors.

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Table 1 The oligonucleotide primers sequence of the studied genes (in vivo)
Table 1 The oligonucleotide primers sequence of the studied genes (in vivo)CC BY · PubMed Central
Table 2 Comparison of the performance in Y-Maze and open field tests in the studied groups at the end of the work
Table 2 Comparison of the performance in Y-Maze and open field tests in the studied groups at the end of the workCC BY · PubMed Central
Table 3 Serum markers of liver function in the studied groups
Table 3 Serum markers of liver function in the studied groupsCC BY · PubMed Central
Table 4 Measured hepatic parameters by gene expression in the studied groups
Table 4 Measured hepatic parameters by gene expression in the studied groupsCC BY · PubMed Central
Table 5 Measured brain parameters by gene expression in the studied groups
Table 5 Measured brain parameters by gene expression in the studied groupsCC BY · PubMed Central
Table 6 Percentage of fibrosis area in liver of the studied groups and Ishak score of each group
Table 6 Percentage of fibrosis area in liver of the studied groups and Ishak score of each groupCC BY · PubMed Central
Figure 1 MSC in culture: A: spindle-shaped (undifferentiated); B: rounded shaped (differentiated)
Figure 1 MSC in culture: A: spindle-shaped (undifferentiated); B: rounded shaped (differentiated)CC BY · PubMed Central
Figure 2 Detection of MCSs homing into liver and brain: I: The presence of red fluorescent indicates homing of differentiated cells into liver (A) and brain (B) tissue; II: The presence of red fluorescent indicate homing of undifferentiated cells into liver (C) and brain (D) tissue.
Figure 2 Detection of MCSs homing into liver and brain: I: The presence of red fluorescent indicates homing of differentiated cells into liver (A) and brain (B) tissue; II: The presence of red fluorescent indicate homing of undifferentiated cells into liver (C) and brain (D) tissue.CC BY · PubMed Central
Figure 3 Photomicrograph of the liver sections stained by H&E (X400): A= Control, B= TAA group, C=u ndifferentiated MSCs group, D= differentiated MSCs group
Figure 3 Photomicrograph of the liver sections stained by H&E (X400): A= Control, B= TAA group, C=u ndifferentiated MSCs group, D= differentiated MSCs groupCC BY · PubMed Central
Figure 4 Photomicrograph of the liver sections stained by MT (x400): A= Control, B= TAA group, C= undifferentiated MSCs group, D= differentiated MSCs group
Figure 4 Photomicrograph of the liver sections stained by MT (x400): A= Control, B= TAA group, C= undifferentiated MSCs group, D= differentiated MSCs groupCC BY · PubMed Central
Figure 5 Photomicrograph of the basal ganglia stained by H&E (X400): A= Control, B= TAA group, C= undifferentiated MSCs group, D= differentiated MSCs group
Figure 5 Photomicrograph of the basal ganglia stained by H&E (X400): A= Control, B= TAA group, C= undifferentiated MSCs group, D= differentiated MSCs groupCC BY · PubMed Central
Figure 6 Photomicrograph of granular and molecular layers of the CA1 region of the hippocampus stained by H&E (X400): A= Control, B= TAA group, C= undifferentiated MSCs group, D= differentiated MSCs group
Figure 6 Photomicrograph of granular and molecular layers of the CA1 region of the hippocampus stained by H&E (X400): A= Control, B= TAA group, C= undifferentiated MSCs group, D= differentiated MSCs groupCC BY · PubMed Central
Figure 7 Photomicrograph of the pyramidal layer of the CA1 region of the hippocampus stained by H&E (X400): A= Control, B= TAA group, C= undifferentiated MSCs group, D= differentiated MSCs group
Figure 7 Photomicrograph of the pyramidal layer of the CA1 region of the hippocampus stained by H&E (X400): A= Control, B= TAA group, C= undifferentiated MSCs group, D= differentiated MSCs groupCC BY · PubMed Central

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  1. [1]Albrecht J Zielińska M Deficit of astroglia-derived thrombospondin-1 and loss of synaptic proteins in hepatic encephalopathy: do ammonia-overexposed astrocytes derange the synaptic hardware? J Neurochem 2014 131 265 267 25105435
  2. [2]Arai K Matsuki N Ikegaya Y Nishiyama N Deterioration of spatial learning performances in lipopolysaccharide-treated mice Jpn J Pharmacol 2001 87 195 201 11885968
  3. [3]Bai YQ Yang YX Yang YG Ding SZ Jin FL Cao MB Outcomes of autologous bone marrow mononuclear cell transplantation in decompensated liver cirrhosis World J Gastroenterol 2014 20 8660 8666 25024623 PMC4093718
  4. [4]Bancroft JD Theory and practice of histological techniques 2008 6th Amsterdam Elsevier Health Sciences
  5. [5]Baykara B Cetin F Baykara B Aksu I Dayi A Kiray M Anxiety caused by traumatic brain injury correlates to decreased prefrontal cortex VEGF immunoreactivity and neuron density in immature rats Turk Neurosurg 2012 22 604 610 23015338
  6. [6]Bear MF Malenka RC Synaptic plasticity: LTP and LTD Curr Opin Neurobiol 1994 4 389 399 7919934
  7. [7]Bélanger M Desjardins P Chatauret N Butterworth RF Loss of expression of glial fibrillary acidic protein in acute hyperammonemia Neurochem Int 2002 41 155 160 12020615
  8. [8]Berg T Rountree CB Lee L Estrada J Sala FG Choe A Fibroblast growth factor 10 is critical for liver growth during embryogenesis and controls hepatoblast survival via beta-catenin activation Hepatology 2007 46 1187 1197 17668871 PMC3494299
  9. [9]Bernardi C Tramontina AC Nardin P Biasibetti R Costa AP Vizueti AF Treadmill exercise induces hippocampal astroglial alterations in rats Neural Plast 2013 2013 709732 23401802 PMC3562665
  10. [10]Bouton ME Moody EW Memory processes in classical conditioning Neurosci Biobehav Rev 2004 28 663–74 15555676
  11. [11]Chakravarthy VS Joseph D Bapi RS What do the basal ganglia do? A modeling perspective Biol Cybern 2010 103 237 253 20644953
  12. [12]Chan YH Biostatistics 103: Qualitative Data –Tests of Independence Singapore Med J 2003 44 498 503 15024452
  13. [13]Chan YH Biostatistics102: Quantitative Data – Parametric & Non-parametric Tests Singapore Med J 2003 44 391 396 14700417
  14. [14]Chang YJ Liu JW Lin PC Sun LY Peng CW Luo GH Mesenchymal stem cells facilitate recovery from chemically induced liver damage and decrease liver fibrosis Life Sci 2009 85 517 525 19686763
  15. [15]Chen SL Zheng MH Shi KQ Yang T Chen YP A new strategy for treatment of liver fibrosis: letting MicroRNAs do the job BioDrugs 2013 27 25 34 23329398
  16. [16]Dalakas E Newsome PN Harrison DJ Plevris JN Hematopoietic stem cell trafficking in liver injury FASEB J 2005 19 1225 1231 16051689
  17. [17]Ding DC Shyu WC Lin SZ Mesenchymal stem cells Cell Transplant 2011 20 5 14 21396235
  18. [19]El-Ghundi M O'Dowd BF George SR Insights into the role of dopamine receptor systems in learning and memory Rev Neurosci 2007 18 37 66 17405450
  19. [21]Hernandez-Gea V Friedman SL Pathogenesis of liver fibrosis Annu Rev Pathol 2011 6 425 456 21073339
  20. [22]Hosseini N Nasehi M Radahmadi M Zarrindast MR Effects of CA1 glutamatergic systems upon memory impairments in cholestatic rats Behav Brain Res 2013 256 636 645 24050889
  21. [23]Hwang S Hong HN Kim HS Park SR Won YJ Choi ST Hepatogenic differentiation of mesenchymal stem cells in a rat model of thioacetamide-induced liver cirrhosis Cell Biol Int 2012 36 279 288 21966929
  22. [24]Ishak K Baptista A Bianchi L Callea F Groote JD Gudat F Histologic grading and staging of chronic hepatitis J Hepatol 1995 24 289 293 7560864
  23. [25]Ishii T Fukumitsu K Yasuchika K Adachi K Kawase E Suemori H Effects of extracellular matrixes and growth factors on the hepatic differentiation of human embryonic stem cells Am J Physiol Gastrointest Liver Physiol 2008 295 G313 G321 18535293
  24. [26]Jang YO Kim YJ Baik SK Kim MY Eom YW Cho MY Histological improvement following administration of autologous bone marrow-derived mesenchymal stem cells for alcoholic cirrhosis: a pilot study Liver Int 2014 34 33 41 23782511
  25. [27]Jayakumar AR Tong XY Curtis KM Ruiz-Cordero R Shamaladevi N Abuzamel M Decreased astrocytic thrombospondin-1 secretion after chronic ammonia treatment reduces the level of synaptic proteins: in vitro and in vivo studies J Neurochem 2014 131 333 347 25040426 PMC4364553
  26. [28]Kalva SK Rengaswamy M Chakravarthy VS Gupte N On the neural substrates for exploratory dynamics in basal ganglia: a model Neural Netw 2012 32 65 73 22386780
  27. [29]Leke R de Oliveira DL Mussulini BH Pereira MS Kazlauckas V Mazzini G Impairment of the organization of locomotor and exploratory behaviors in bile duct-ligated rats PLoS One 2012 7 e36322 22586467 PMC3346757
  28. [30]Lin J Wu JF Zhang Q Zhang HW Cao GW Virus-related liver cirrhosis: molecular basis and therapeutic options World J Gastroenterol 2014 20 6457 6469 24914367 PMC4047331
  29. [31]Lysy PA Campard D Smets F Najimi M Sokal EM Stem cells for liver tissue repair: current knowledge and perspectives World J Gastroenterol 2008 14 864 875 18240343 PMC2687053
  30. [32]Madro A Czechowska G Slomka M Celinski K Szymonik-Lesiuk S Kurzepa J The decrease of serum MMP-2 activity corresponds to alcoholic cirrhosis stage Alcohol 2012 46 155 157 21908153
  31. [33]Mladenović D Hrnčić D Rašić-Marković A Stanojlović Olivera Behavioral and electroencephalographic manifestations of thioacetamide-induced encephalopathy: Possible mechanisms of neurotoxic effects Arch Biol Sci 2012 64 829 841 22913436
  32. [34]Mohsin S Shams S Ali Nasir G Khan M Javaid Awan S Khan SN Enhanced hepatic differentiation of mesenchymal stem cells after pretreatment with injured liver tissue Differentiation 2011 81 42 48 20943307
  33. [35]Mormone E George J Nieto N Molecular pathogenesis of hepatic fibrosis and current therapeutic approaches Chem Biol Interact 2011 193 225 231 21803030 PMC3171510
  34. [36]Nasehi M Amin YS Zarrindast MR Synergistic effects between CA1 mu opioid and dopamine D1-like receptors in impaired passive avoidance performance induced by hepatic encephalopathy in mice Psychopharmacology (Berl) 2013 227 553 566 23404062
  35. [37]Norton NS McConnell JR Rodriguez-Sierra JF Behavioral and physiological sex differences observed in an animal model of fulminant hepatic encephalopathy in the rat Physiol Behav 1997 62 1113 1124 9333208
  36. [38]Oyagi S Hirose M Kojima M Okuyama M Kawase M Nakamura T Therapeutic effect of transplanting HGF-treated bone marrow mesenchymal cells into CCl4-injured rats J Hepatol 2006 44 742 748 16469408
  37. [39]Parekkadan B Poll D Suganuma K Carter EA Berthiaume F Tilles AW Mesenchymal stem cell-derived molecules reverse fulminant hepatic failure PLoS One 2007 2 941 PMC1978513 17895982
  38. [40]Parekkadan B van Poll D Megeed Z Kobayashi N Tilles AW Berthiaume F Immunomodulation of activated hepatic stellate cells by mesenchymal stem cells Biochem Biophys Res Commun 2007 363 247 252 17869217 PMC2096777
  39. [41]Park SY Shin HW Lee KB Lee MJ Jang JJ Differential expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in thioacetamide-induced chronic liver injury J Korean Med Sci 2010 25 570 576 20358000 PMC2844613
  40. [42]Peeling J Shoemaker L Gauthier T Benarroch A Sutherland GR Minuk GY Cerebral metabolic and histological effects of thioacetamide-induced liver failure Am J Physiol 1993 265 G572 G578 8214078
  41. [43]Pei L Lee FJ Moszczynska A Vukusic B Liu F Regulation of dopamine D1 receptor function by physical interaction with the NMDA receptors J Neurosci 2004 24 1149 1158 14762133 PMC6793575
  42. [44]Piryaei A Valojerdi MR Shahsavani M Baharvand H Differentiation of bone marrow-derived mesenchymal stem cells into hepatocyte-like cells on nanofibers and their transplantation into a carbon tetrachloride-induced liver fibrosis model Stem Cell Rev 2011 7 103 118 20182823
  43. [45]Poh Z Chang PE A Current review of the diagnostic and treatment strategies of hepatic encephalopathy Int J Hepatol 2012 2012 480309 23133760 PMC3485515
  44. [47]Puche JE Saiman Y Friedman SL Hepatic stellate cells and liver fibrosis Compr Physiol 2013 3 1473 1492 24265236
  45. [48]Reza Zarrindast M Eslimi Esfahani D Oryan S Nasehi M Torabi Nami M Effects of dopamine receptor agonist and antagonists on cholestasis-induced anxiolytic-like behaviors in rats Eur J Pharmacol 2013 702 25 31 23376414
  46. [49]Romero-Gomez M Cordoba J Jover R del Olmo JA Ramirez M Rey R Value of the critical flicker frequency in patients with minimal hepatic encephalopathy Hepatology 2007 45 879 885 17393525
  47. [50]Salama H Zekri AR Medhat E Al Alim SA Ahmed OS Bahnassy AA Peripheral vein infusion of autologous mesenchymal stem cells in Egyptian HCV-positive patients with end-stage liver disease Stem Cell Res Ther 2014 5 3 70 24886681 PMC4097846
  48. [51]Salama SM Abdulla MA AlRashdi AS Ismail S Alkiyumi SS Golbabapour S Hepatoprotective effect of ethanolic extract of Curcuma longa on thioacetamide induced liver cirrhosis in rats BMC Complement Altern Med 2013 13 56 23496995 PMC3605171
  49. [52]Schuppan D Afdhal NH Liver cirrhosis Lancet 2008 371 838 851 18328931 PMC2271178
  50. [54]Shi M Liu ZW Wang FS Immunomodulatory properties and therapeutic application of mesenchymal stem cells Clin Exp Immunol 2011 164 1 8 PMC3074211 21352202
  51. [55]Singal AK Duchini A Liver transplantation in acute alcoholic hepatitis: current status and future development World J Hepatol 2011 3 215–8 21954410 PMC3180607
  52. [56]Sun F Hayami S Ogiri Y Evaluation of oxidative stress based on lipid hydroperoxide, vitamin C and vitamin E during apoptosis and necrosis caused by thioacetamide in rat liver Biochim Biophys Acta 2000 1500 181–5 10657587
  53. [58]Volarevic V Nurkovic J Arsenijevic N Stojkovic M Concise review: Therapeutic potential of mesenchymal stem cells for the treatment of acute liver failure and cirrhosis Stem Cells 2014 32 2818 2823 25154380
  54. [59]Wang J Bian C Liao L Zhu Y Li J Zeng L Inhibition of hepatic stellate cells proliferation by mesenchymal stem cells and the possible mechanisms Hepatol Res 2009 39 1219 1228 19788697
  55. [60]Xu H Qian H Zhu W Zhang X Yan Y Mao F Mesenchymal stem cells relieve fibrosis of Schistosoma japonicum-induced mouse liver injury Exp Biol Med (Maywood) 2012 237 585 592 22678013
  56. [61]Yang J Zheng J Wu L Shi M Zhang H Wang X NDRG2 ameliorates hepatic fibrosis by inhibiting the TGF-β1/Smad pathway and altering the MMP2/ TIMP2 ratio in rats PLoS One 2011 6 27710 PMC3218018 22110735
  57. [62]Zhang XM Du F Yang D Yu CJ Huang XN Liu W Transplanted bone marrow stem cells relocate to infarct penumbra and co-express endogenous proliferative and immature neuronal markers in a mouse model of ischemic cerebral stroke BMC Neurosci 2010 11 38 20973978 PMC2974740
  58. [63]Zhao W Li JJ Cao DY Li X Zhang LY He Y Intravenous injection of mesenchymal stem cells is effective in treating liver fibrosis World J Gastroenterol 2012 18 1048 1058 22416179 PMC3296978

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Metadata

Authors
Elberry, D. A., Amin, S. N., Esmail, R. S. E. N., Rashed, L. A., & Gamal, M. M.
Affiliations
  • Cairo University (EG)
  • Fayoum University (EG)
ORCID
Venue
IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund
Publisher
IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund
Published
2016
Volume
15
Pages
652-670
ISSN
1611-2156 (ISSN-L)
DOI
10.17179/excli2016-645
PMC
PMC5318675
OpenAlex
W2595197567
Cited by
12
References
1
Citations by year
2025: 1 · 2024: 3 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 1 · 2018: 2
FWCI
0.48
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • EXCLI journal (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Technische Universität Dortmund Eldorado (Technische Universität Dortmund) (submittedVersion, cc-by)
  • Leibniz-Institut für Arbeitsforschung an der TU Dortmund (IfADo)
  • Technische Universität Dortmund Eldorado (Technische Universität Dortmund) (cc-by)
  • PubMed
Funders
Cairo University
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
  • Health Sciences › Medicine › Hepatology › Liver Disease and Transplantation
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
Keywords
mesenchymal stem cells, liver cirrhosis, hepatic encephalopathy, hepatocyte growth factor, stem cell differentiation, cognitive function, thioacetamide-induced liver injury, apoptosis, glial fibrillary acidic protein, synaptophysin, dopamine receptors, rat model

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CoChat. (2026). Citation check: Effect of undifferentiated versus hepatogenic partially differentiated mesenchymal stem cells on hepatic and cognitive functions in liver cirrhosis. https://app.cochat.ai/papers/10.17179/excli2016-645
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Elberry, D. A., Amin, S. N., Esmail, R. S. E. N., Rashed, L. A., & Gamal, M. M. (2016). Effect of undifferentiated versus hepatogenic partially differentiated mesenchymal stem cells on hepatic and cognitive functions in liver cirrhosis. IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund. https://doi.org/10.17179/excli2016-645