A ketogenic diet impacts markers of mitochondrial mass in a tissue specific manner in aged mice
Open accessAbstract
Declines in mitochondrial mass are thought to be a hallmark of mammalian aging, and a ketogenic diet (KD) may prevent the age-related decreases in mitochondrial content. The objective of this study was to investigate the impact of a KD on markers of mitochondrial mass. Mice were fed an isocaloric control diet (CD) or KD from 12 months of age. Tissues were collected after 1 month and 14 months of intervention, and a panel of commonly used markers of mitochondrial mass (mitochondrial enzyme activities and levels, mitochondrial to nuclear DNA ratio, and cardiolipin content) were measured. Our results showed that a KD stimulated activities of marker mitochondrial enzymes including citrate synthase, Complex I, and Complex IV in hindlimb muscle in aged mice. KD also increased the activity of citrate synthase and prevented an age-related decrease in Complex IV activity in aged brain. No other markers were increased in these tissues. Furthermore, the impacts of a KD on liver and kidney were mixed with no pattern indicative of a change in mitochondrial mass. In conclusion, results of the present study suggest that a KD induces tissue-specific changes in mitochondrial enzyme activities, or structure, rather than global changes in mitochondrial mass across tissues.
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Long-term exposure to a ketogenic diet increases markers of mitochondrial content in aged skeletal muscles.
“and increased markers of mitochondrial content in aged skeletal muscles [174]”
From Mitochondrial Dynamics and Mitophagy in Skeletal Muscle Health and Aging (2021) · cites this paper as [174]
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References 53 · Cited by 19
- Authors
- Zhou, Z., Hagopian, K., López-Domínguez, J. A., Kim, K., Jasoliya, M., Roberts, M. N., Cortopassi, G. A., Showalter, M. R., Roberts, B. S., González-Reyes, J. A., Baar, K., Rutkowsky, J., & Ramsey, J. J.
- Affiliations
- University of California, Davis (US)
- Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, CA 95616, USA
- University of Córdoba (ES)
- Corresponding author
- Rutkowsky, Jennifer
- Ramsey, Jon J.
- ORCID
- Venue
- Aging
- Publisher
- Impact Journals, LLC
- Published
- 2021-03-18
- Volume
- 13
- Issue
- 6
- Pages
- 7914-7930
- ISSN
- 1945-4589 (ISSN-L)
- PMC
- PMC8034930
- OpenAlex
- W3139304068
- Cited by
- 19
- References
- 53
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 3 · 2023: 4 · 2022: 5 · 2021: 3
- FWCI
- 2.36
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by
- Locations
- Aging (publishedVersion)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- eScholarship (California Digital Library) (submittedVersion, cc-by)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- National Institutes of Health (PO1 AG062817)
- National Institute on Aging (P01AG062817)
- Topics
- Health Sciences › Medicine › Physiology › Diet and metabolism studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Keywords
- ketogenic diet, mitochondrial mass, mitochondrial enzyme activity, citrate synthase, mitochondrial complex I, mitochondrial complex IV, cardiolipin, hindlimb muscles, brain mitochondria, tissue-specific effects, aged mice
- MeSH terms
- Animals, Electron Transport Complex IV, Kidney, Liver, Male, Mitochondria, Electron Transport Complex I, Mice, Diet, Ketogenic
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