The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
MULTILOCUS SEQUENCE TYPING OF CARBAPENEM RESISTANT PSEUDOMONAS AERUGINOSA ISOLATES FROM PATIENTS PRESENTING AT PORT ELIZABETH HOSPITALS, SOUTH AFRICA
Open accessAbstract
Background: Pseudomonas aeruginosa is an important nosocomial pathogen that exhibits multiple drug resistance with increasing frequency, especially to carbapenems making patient treatment difficult.Carbapenem-resistance may be caused by porin gene mutations, active drug efflux, and carbapenemase production.This study evaluated the incidence of genes responsible for carbapenemase production in carbapenem-resistant Pseudomonas aeruginosa and assessed the genetic relatedness of the isolates by multi locus sequence typing (MLST).Materials and Methods: Identification and antimicrobial susceptibility testing of P. aeruginosa isolates (n=234) by the VITEK 2 system detected 81 carbapenem resistant P. aeruginosa isolates.PCR and DNA sequencing were used to screen isolates for three metallo-β-lactamase encoding genes.MLST included amplification of seven housekeeping genes and sequence type alignment using the online P. aeruginosa MLST database.Results: Only the blaVIM-2 gene was detected in 15 of the 81 carbapenem resistant isolates.MLST indicated six different novel sequence types among the blaVIM-2 positive P. aeruginosa isolates with the majority of the isolates (9/15) containing identical allelic profiles of the sequence type allocated ST1 (provisionally assigned sequence type, awaiting addition of new sequence types to PubMLST database).Five of these ST1 isolates were from patients and an environmental sample in the same hospital ward suggesting an environmental reservoir.Carbapenem resistance in the blaVIM-2 negative isolates may be due to other mechanisms.Conclusion: The incidence of genes responsible for carbapenemase production in carbapenem-resistant Pseudomonas aeruginosa and genetic relatedness of these isolates in public healthcare facilities within the Port Elizabeth area is of concern and requires further investigation.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The check wasn’t confident either way, so it gives no verdict — never disputed.
In the citing paper
“The cooccurrence of class I and class II integrons has also been reported, which possess a variety of resistance genes, such as ant(4′)-IIb, dfrA, aadAB, qnrVC1, gyrA, and parC.147,177,179,181,182,[184][185][186][187]”
From Antimicrobial Resistance Rates and Surveillance in Sub-Saharan Africa: Where Are We Now? (2022) · cites this paper as [179]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Carbapenemases: the Versatile β-Lactamases
Queenan, A. M., & Bush, K. (2007) · Clinical Microbiology Reviews · doi:10.1128/cmr.00001-07 · Cited by 2,609
Global Spread of Carbapenemase-producing Enterobacteriaceae
Nordmann, P., Naas, T., & Poirel, L. (2011) · Emerging infectious diseases · doi:10.3201/eid1710.110655 · Cited by 2,489
The Epidemiology, Pathogenesis and Treatment of Pseudomonas aeruginosa Infections
Driscoll, J. A., Brody, S. L., & Kollef, M. H. (2007) · Drugs · doi:10.2165/00003495-200767030-00003 · Cited by 911
Multiplex PCR for rapid detection of genes encoding acquired metallo- -lactamases
Ellington, M. J., Kistler, J., Livermore, D. M., & Woodford, N. (2006) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkl481 · Cited by 608
Curran, B., Jonas, D., Grundmann, H., Pitt, T. L., & Dowson, C. G. (2004) · Journal of Clinical Microbiology · doi:10.1128/jcm.42.12.5644-5649.2004 · Cited by 526
Hirsch, E. B., & Tam, V. H. (2010) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkq108 · Cited by 482
Molecular Epidemiology and Mechanisms of Carbapenem Resistance in Pseudomonas aeruginosa
Rodríguez-Martínez, J. M., Poirel, L., & Nordmann, P. (2009) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00574-09 · Cited by 307
Riera, E., Cabot, G., Mulet, X., García-Castillo, M., del Campo, R., Juan, C., Cantón, R., & Oliver, A. (2011) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkr232 · Cited by 189
Edelstein, M. V., Skleenova, E. Y., Shevchenko, O. V., D'souza, J. W., Tapalski, D. V., Azizov, I., Витальевна, С. М., Pavlukov, R. A., Kozlov, R. S., Toleman, M. A., et al. (2013) · The Lancet Infectious Diseases · doi:10.1016/s1473-3099(13)70168-3 · Cited by 176
Ertapenem, the first of a new group of carbapenems
SHAH, P. M. (2003) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkg404 · Cited by 158
Pavón, A. B. I., & Maiden, M. C. J. (2009) · Methods in molecular biology · doi:10.1007/978-1-60327-999-4_11 · Cited by 93
Baumgart, A. M. K., Molinari, M. A., & Silveira, A. C. D. O. (2010) · The Brazilian Journal of Infectious Diseases · doi:10.1016/s1413-8670(10)70089-1 · Cited by 55
Mudau, M., Jacobson, R. K., Minenza, N., Kuonza, L. R., Morris, V., Engelbrecht, H., Nicol, M. P., & Bamford, C. (2013) · PLoS ONE · doi:10.1371/journal.pone.0055985 · Cited by 54
Genetic diversity of clinical Pseudomonas aeruginosa isolates in a public hospital in Spain
Gomila, M., Gallegos, M. D. C., Fernández‐Baca, V., Pareja, A., Pascual, M., Díaz‐Antolín, P., Garcı́a-Valdés, E., & Lalucat, J. (2013) · BMC Microbiology · doi:10.1186/1471-2180-13-138 · Cited by 48
VIM-2 metallo- -lactamase-producing Pseudomonas aeruginosa causing an outbreak in South Africa
Jacobson, R. K., Minenza, N., Nicol, M. P., & Bamford, C. (2012) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dks100 · Cited by 42
Meletis, G., Vavatsi, N. A., Exindari, M., Protonotariou, E., Sianou, E., Haitoglou, C., Sofianou, D., Pournaras, S., & Diza, E. (2013) · European Journal of Clinical Microbiology & Infectious Diseases · doi:10.1007/s10096-013-1952-3 · Cited by 34
Cho, H. H., Kwon, G. C., Kim, S., & Koo, S. H. (2015) · Journal of Microbiology and Biotechnology · doi:10.4014/jmb.1503.03065 · Cited by 28
VIM-2 carbapenemase-producing Pseudomonas aeruginosa in a patient from Port Elizabeth, South Africa
Govender, S., Masunda, T., & Black, J. M. (2015) · South African Medical Journal · doi:10.7196/samj.9513 · Cited by 6
Molecular Typing Methods for the Genus Pseudomonas
Libisch, B. (2012) · Humana Press eBooks · doi:10.1007/978-1-62703-185-1_24 · Cited by 4
Gqunta, K. (2014) · Nelson Mandela University Research Repository · Cited by 1
Cited by
Antimicrobial Resistance Rates and Surveillance in Sub-Saharan Africa: Where Are We Now?
Kariuki, S., Kering, K., Wairimu, C., Onsare, R., & Mbae, C. (2022) · Infection and Drug Resistance · doi:10.2147/idr.s342753 · Cited by 245
Tahmasebi, H., Dehbashi, S., Nasaj, M., & Arabestani, M. R. (2022) · Scientific Reports · doi:10.1038/s41598-022-11984-1 · Cited by 25
Tahmasebi, H., Dehbashi, S., & Arabestani, M. R. (2020) · Infection Genetics and Evolution · doi:10.1016/j.meegid.2020.104518 · Cited by 23
Lob, S. H., Estabrook, M. A., DeRyke, C. A., Alekseeva, I., Siddiqui, F., Young, K., Motyl, M. R., & Sahm, D. F. (2022) · International Journal of Infectious Diseases · doi:10.1016/j.ijid.2022.10.014 · Cited by 15
Choonara, F., Haldorsen, B. C., Ndhlovu, I., Saulosi, O., Maida, T., Lampiao, F., Simonsen, G. S., Essack, S. Y., & Sundsfjord, A. (2022) · Malawi Medical Journal · doi:10.4314/mmj.v34i1.3 · Cited by 10
Metadata
- Authors
- D, A., J, B., & S, G.
- Venue
- African Journal of Infectious Diseases
- Published
- 2017-06-08
- Volume
- 11
- Issue
- 2
- Pages
- 68-74
- ISSN
- 2006-0165 (ISSN-L)
- 2505-0419
- PMC
- PMC5476815
- OpenAlex
- W2622307274
- Cited by
- 11
- References
- 20
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2022: 5 · 2020: 1 · 2019: 1
- FWCI
- 0.21
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- African Journal of Infectious Diseases (publishedVersion)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Nelson Mandela University Research Repository (submittedVersion)
- Funders
- Nelson Mandela Metropolitan University
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- National Health Laboratory Service
- Nelson Mandela University
- Topics
- Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
- Health Sciences › Medicine › Microbiology (medical) › Infections and bacterial resistance
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Keywords
- Pseudomonas aeruginosa, carbapenem resistance, multilocus sequence typing, blaVIM-2, metallo-beta-lactamase, sequence types, nosocomial infections, environmental reservoirs, antimicrobial resistance
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
