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Cited by 433D Printing in Biomedical ResearchGut microbiota and healthMicrofluidic and Capillary Electrophoresis Applications

Gut-microbiota-on-a-chip: an enabling field for physiological research

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Trujillo-de Santiago, Grissel, Lobo-Zegers, Matías José, Montes-Fonseca, Silvia Lorena, Zhang, Yu Shrike, Alvarez, Mario Moisés·2018·Microphysiological Systems
DOI 10.21037/mps.2018.09.01PMC PMC8096182
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Abstract

Overwhelming scientific evidence today confirms that the gut microbiota is a central player in human health. Knowledge about interactions between human gut microbiota and human health has evolved rapidly in the last decade, based on experimental work involving analysis of human fecal samples or animal models (mainly rodents). A more detailed and cost-effective description of this interplay is now being enabled by the use of in vitro systems (i.e., gut-microbiota-on-chip systems) that recapitulate key aspects of the interaction between microbiota and human cells. Here, we review recent examples of the design and use of pioneering on-chip platforms for the study of the cross-talk between representative members of human microbiota and human microtissues. In these systems, the combined use of state-of-the-art microfluidics, biomaterials, cell culture techniques, classical microbiology, and a touch of genetic expression profiling have converged for the development of gut-on-chip platforms capable of recreating key features of the interplay between human microbiota and host human tissues. We foresee that the integration of novel microfabrication techniques and stem cell technologies will further accelerate the development of more complex and physiologically relevant microbiota-on-chip platforms. In turn, this will foster the faster acquisition of knowledge regarding human microbiota and will enable important advances in the understanding of how to control or prevent disease.

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Authors
Trujillo-de Santiago, G., Lobo-Zegers, M. J., Montes-Fonseca, S. L., Zhang, Y. S., & Alvarez, M. M.
Affiliations
  • Tecnológico de Monterrey (MX)
  • Brigham and Women's Hospital (US)
ORCID
Venue
Microphysiological Systems
Publisher
AME Publishing Company
Published
2018
Volume
1
Pages
1-1
ISSN
2616-275X (ISSN-L)
DOI
10.21037/mps.2018.09.01
PMC
PMC8096182
OpenAlex
W2895944563
Cited by
43
References
126
Citations by year
2025: 5 · 2024: 9 · 2023: 5 · 2022: 6 · 2021: 7 · 2020: 8 · 2019: 3
FWCI
1.80
Type
journal article
Language
en
Open access
Licence
cc-by-nc-nd
Locations
  • Microphysiological Systems (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Cancer Institute (K99CA201603)
  • National Institute of Biomedical Imaging and Bioengineering (R21EB025270)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
Keywords
gut microbiota, gut-on-a-chip, microbiota-host interactions, microfluidics, microtissues, in vitro model, biomaterials, stem cell technology, disease prevention, human health

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