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PreprintCited by 4Bacteriophages and microbial interactionsEscherichia coli research studiesBacterial Genetics and Biotechnology

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Isolation, characterization, and receptor-binding protein specificity of phages PAS7, PAS59 and PAS61 infecting Shiga toxin-producing Escherichia coli O103 and O146

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Pas, Célia, Fieseler, Lars, Pothier, Joël F., Briers, Yves·2024·Scientific Reports
DOI 10.21203/rs.3.rs-4758770/v1PMC PMC11522302

Preprint — published as 10.1038/s41598-024-77463-x · Paper page

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Abstract

Shiga toxin-producing Escherichia coli (STEC) is a foodborne pathogen with 6,534 annual reported cases in the EU in 2021. This pathotype generally contains strains with smooth LPS with O-antigen serogroup O157 being the predominant serogroup in the US. However, non-O157 STEC serogroups are becoming increasingly prevalent. Here we announce the complete genomes of three newly isolated phages that infect STEC serogroups O103 and O146, namely Escherichia phages vB_EcoP_PAS7, vB_EcoP_PAS59 and vB_EcoP_PAS61. The genome sequences revealed that they belong to three distinct genera, namely the newly proposed genus Cepavirus within the Slopekvirinae subfamily, the genus Yesanvirus and the genus Uetakevirus, respectively. We identified the tailspikes of phages PAS7 and PAS61 as a primary specificity determinant for the O-antigens O103 and O146, respectively, and predicted their active site in silico.

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Metadata

Authors
Pas, C., Fieseler, L., Pothier, J. F., & Briers, Y.
Affiliations
  • Ghent University (BE)
  • ZHAW Zurich University of Applied Sciences (CH)
Corresponding author
Briers, Yves
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2024-08-17
Volume
14
Issue
1
Pages
26050-26050
ISSN
2045-2322 (ISSN-L)
DOI
10.21203/rs.3.rs-4758770/v1
PMC
PMC11522302
OpenAlex
W4403892317
Cited by
4
References
1
Citations by year
2026: 2 · 2025: 2
FWCI
1.10
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by-nc-nd)
  • Research Square (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • Ghent University Academic Bibliography (Ghent University) (submittedVersion, cc-by-nc-nd)
  • Zürcher Hochschule für Angewandte Wissenschaften digital collection (Zurich University of Applied Sciences) (submittedVersion, cc-by-nc-nd)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • ZHAW Digital Collection
Funders
  • Fonds Wetenschappelijk Onderzoek (1S79422N)
  • Universität Zürich
  • Norges Miljø- og Biovitenskapelige Universitet
  • Vlaamse regering
  • Zürcher Hochschule für Angewandte Wissenschaften
Topics
  • Life Sciences › Immunology and Microbiology › Virology › Bacteriophages and microbial interactions
  • Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Shiga toxin-producing Escherichia coli, bacteriophages, phage genome, receptor specificity, O-antigen, phage isolation, non-O157 STEC
MeSH terms
Bacteriophages, Coliphages, Phylogeny, Genome, Viral, O Antigens, Shiga-Toxigenic Escherichia coli, Host Specificity

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