The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
MYCL promotes iPSC-like colony formation via MYC Box 0 and 2 domains
Open accessPreprint — published as 10.1038/s41598-021-03260-5 · Paper page
Abstract
Induced pluripotent stem cells (iPSCs) have the potential to differentiate into any cell in the body and thus have attractive regenerative medicine potential. Current iPSC generation protocols, however, have low efficiency and show variable quality among clones. This variability influences the efficiency and reproducibility of iPSC differentiation. Our previous study reported that MYC proteins (c-MYC and MYCL) are important for the reprogramming efficiency and germline transmission of iPSCs, but that MYCL can generate iPSC colonies more efficiently than c-MYC. However, why c-MYC and MYCL cause different reprogramming efficiencies is unknown. In this study, we found that MYC Box 0 (MB0) and MB2, two functional domains conserved in the MYC protein family, contribute to the phenotypic difference and promote iPSC generation in MYCL-induced reprogramming. Proteome analysis suggested that in MYCL-induced reprogramming, cell adhesion-related cytoskeletal proteins are regulated by the MB0 domain, while RNA processes are regulated by the MB2 domain. These findings provide a molecular explanation for why MYCL has higher reprogramming efficiency than c-MYC.
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Cites
Global quantification of mammalian gene expression control
Schwanhäusser, B., Busse, D., Li, N., Dittmar, G., Schuchhardt, J., Wolf, J., Chen, W., & Selbach, M. (2011) · Nature · doi:10.1038/nature10098 · Cited by 6,566
Nakagawa, M., Taniguchi, Y., Senda, S., Takizawa, N., Ichisaka, T., Asano, K., Morizane, A., Doi, D., Takahashi, J., Nishizawa, M., et al. (2014) · Scientific Reports · doi:10.1038/srep03594 · Cited by 673
Xing, Q. R., El Farran, C. A., Gautam, P., Chuah, Y. S., Warrier, T., Toh, C. X. D., Kang, N. Y., Sugii, S., Chang, Y. T., Xu, J., et al. (2020) · Science Advances · doi:10.1126/sciadv.aba1190 · Cited by 51
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Metadata
- Authors
- Akifuji, C., Iwasaki, M., Kawahara, Y., Sakurai, C., Cheng, Y., Imai, T., & Nakagawa, M.
- Affiliations
- Kyoto University (JP)
- Corresponding author
- Nakagawa, Masato
- Venue
- Research Square
- Publisher
- Research Square Platform LLC
- Published
- 2021-08-19
- Volume
- 11
- OpenAlex
- W3195340497
- Cited by
- 0
- References
- 3
- Type
- posted content
- Language
- en
- Open access
- green
- doi.org
- Locations
- Research Square (acceptedVersion, cc-by)
- Institutional Repositories DataBase (IRDB) (submittedVersion)
- Kyoto University Research Information Repository (Kyoto University) (submittedVersion, cc-by)
- Kyoto University Research Information Repository (Kyoto University) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Keywords
- MYCL, induced pluripotent stem cells, reprogramming efficiency, c-Myc, cell adhesion, cytoskeletal proteins, RNA processing, proteomics
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