In Vitro Antimicrobial Activity and Aggregation Abilities of Probiotic Lactobacillus casei and Lactobacillus salivarius Against Oral Pathogens
Open accessAbstract
The antagonistic effect of probiotics against oral pathogens merits exploration because these bacteria are beneficial to the host’s health. The antimicrobial activity of two probiotic strains, Lactobacillus casei and Lactobacillus salivarius, as well as L. casei and L. salivarius combination (1:1), was investigated against Streptococcus mutans, Streptococcus sobrinus, Candida albicans, Candida glabrata and Candida tropicalis using agar-well diffusion, auto-aggregation and coaggregation assays. L. salivarius cell-free supernatant (CFS) alone exhibited greater inhibitory effect against Streptococci spp. compared to L. casei CFS alone and the combination. However, no inhibition was observed for Candida spp. L. salivarius alone exhibited significantly stronger auto-aggregation than L. casei alone (p ≤ 0.05) and L. casei and L. salivarius combination. L. salivarius exhibited strong coaggregation ability with Candida spp., followed by Streptococci spp. while L. casei exhibited coaggregation only with Streptococci spp. However, L. casei and L. salivarius combination did not display any coaggregation with all strains. L. salivarius alone exhibited a stronger antagonistic effect on the tested organisms than L. casei alone or in combination. Based on the results, both probiotic strains showed good antimicrobial activities against oral pathogens and should be further studied for their human health benefits.
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Selected bacterial strains belonging to the genera Lactobacillus or Bifidobacterium have demonstrated a capacity to aggregate with oral pathogens such as Fusobacterium nucleatum or Streptococcus mutans in vitro and in vivo.
“Selected bacterial strains belonging to the genera Lactobacillus or Bifidobacterium have been applied as probiotics and have demonstrated a capacity to aggregate with oral pathogens, such as Fusobacterium nucleatum or Streptococcus mutans, in vitro [8–10] and in vivo [11].”
From Coaggregation of oral pathogens by postbiotic lactobacilli (2025) · cites this paper as [10]
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References 35 · Cited by 3
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- nu9111242 · DOI
- Authors
- Thayalan, D. T., Abdullah, R., Md Noor, S. S., & Mohamad, S.
- Affiliations
- Universiti Sains Malaysia (MY)
- Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
- Corresponding author
- Mohamad, Suharni
- ORCID
- Mohamad, Suharni
- Venue
- Archives of Orofacial Sciences
- Publisher
- Universiti Sains Malaysia
- Published
- 2021-12-22
- Volume
- 16
- Issue
- 2
- Pages
- 153-163
- ISSN
- 1823-8602 (ISSN-L)
- 2231-7163
- OpenAlex
- W4200538568
- Cited by
- 3
- References
- 35
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 1
- FWCI
- 0.30
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Archives of Orofacial Sciences (publishedVersion, cc-by)
- Funders
- Universiti Sains Malaysia
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Keywords
- probiotics, Lactobacillus casei, Lactobacillus salivarius, antimicrobial activity, oral pathogens, Streptococcus mutans, Streptococcus sobrinus, Candida albicans, Candida glabrata, Candida tropicalis, self-aggregation, co-aggregation, cell-free supernatant, antagonistic effect, dental caries, oral candidiasis
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