CoChat
Sign Up
Cited by 35Sperm and Testicular FunctionAntioxidant Activity and Oxidative StressSmoking Behavior and Cessation

Low‐dose nicotine exposure induced the oxidative damage of reproductive organs and altered the sperm characteristics of adolescent male rats

Open access
School of Diagnostic and Applied Health Science, Faculty of Health Science, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia, Budin, Siti Balkis, Jia Hui, Kho, School of Diagnostic and Applied Health Science, Faculty of Health Science, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia, Jia Hui, Lee, School of Diagnostic and Applied Health Science, Faculty of Health Science, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia, RamaLingam, Anand, School of Diagnostic and Applied Health Science, Faculty of Health Science, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia +10 more·2017·Malaysian Journal of Medical Sciences
DOI 10.21315/mjms2017.24.6.6PMC PMC5771515
Open this paper in CoChat — free account

Abstract

Background: Nicotine is a major toxic and hazardous component of cigarette smoke, and it has been widely used in nicotine replacement therapy (NRT).This study was aimed to investigate the effects of chronic low-dose nicotine on sperm characteristics and reproductive organ integrity in adolescent male Sprague-Dawley rats.Methods: Twelve rats were equally divided into two groups.Group I received normal saline, and group II received 0.6 mg/kg body weight nicotine intraperitoneally for 28 consecutive days.At the end of the experimental period, sperm was collected for sperm characteristic evaluation, and the testes and prostate were isolated for biochemical and morphological analysis.The effects of nicotine on the body and reproductive organ weights of the animals were evaluated.Results: Chronic nicotine treatment significantly (P < 0.05) altered the sperm count, motility, viability, and morphology, and remarkably increased the malondialdehyde (P < 0.001) and advanced oxidation protein product (P < 0.05) levels in the testes and prostate of nicotinetreated group compared to control group.Moreover, nicotine caused a significant decrease (P < 0.05) in the superoxide dismutase activity of the testes.No significant differences were observed in the reduced glutathione level in both of the testes and prostate of nicotine group compared with control group.Nicotine also induced histopathological alteration in the testes.Conclusion: A low-dose nicotine exposure at 0.6 mg/kg caused detrimental effects on sperm characteristics and induced oxidative stress in the testes and prostate.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.21315/mjms2017.24.6.6

Cites

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Experimental studies speak in a similar direction regarding decreased sperm counts after nicotine exposure.

    “which however speak in a similar direction regarding decreased sperm counts after nicotine exposure. 9 , 10”

    From Decreased sperm counts in Swedish users of oral tobacco (2022) · cites this paper as [9]

  • Animal studies have reported that nicotine affected semen parameters in rats, including their sperm counts.

    “Further, animal studies have reported that nicotine affected semen parameters in rats, including their sperm counts. 9 , 10”

    From Decreased sperm counts in Swedish users of oral tobacco (2022) · cites this paper as [9]

  • A study in rats reported impaired sperm parameters after treatment with nicotine.

    “a study in rats reported impaired sperm parameters after treatment with nicotine. 9”

    From Decreased sperm counts in Swedish users of oral tobacco (2022) · cites this paper as [9]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

No references could be resolved yet.

Research works printed but not linked: 30

  1. Favaro WJ, Carvalho CA. Morphometric and morphological features of the ventral prostate in rats submitted to chronic nicotine and alcohol treatment. Tissue Cell. 2006;38(5):311-323. https://doi.org/10.1111/j.1439-0272.2006.00728.x · DOI
  2. Lopes S, Sun JG, Jurisicova A, Meriano J, Casper RF. Sperm deoxyribonucleic acid fragmentation is increased in poor-quality semen samples and correlates with failed fertilization in intracytoplasmic sperm injection. Fertil Steril. 1998;69(3):528-532. https://doi.org/10.1016/ S0015-0282(97)00536-0 · DOI
  3. West R, Hajek P, Foulds J, Nilsson F, May S, Meadows A. A comparison of the abuse liability and dependence potential of nicotine patch, gum, spray and inhaler. Psychopharmacology. 2000;149(3):198-202. https://doi.org/10.1007/ s002130000382 · DOI
  4. Benowitz NL, Jacob P. Daily intake of nicotine during cigarette smoking. Clin Pharmacol Therap. 1984;35(4):499-504. https://doi.org/ 10.1038/clpt.1984.67 · DOI
  5. Toklu H, Ozer S, Hulya S, Sule C, Berrak C, Yegen-Goksel S. Resveratrol supplementation protects against chronic nicotine-induced oxidative damage and organ dysfunction in the rat urogenital system. Marmara Pharm J. 2010;14(1). https://doi.org/10.1038/clpt.1984.67 · DOI
  6. Stocks J, Dormandy T. The autoxidation of human red cell lipids induced by hydrogen peroxide. Br J Haematol. 1971;20(1):95-111. https://doi.org/10.1111/j.1365-2141.1971. tb00790.x · DOI
  7. Witko V, Nguyen AT, Descamps LB. Microtiter plate assay for phagocyte-derived Taurine- chloramines. J Clin Lab Anal. 1992;6(1):47-53. PMID: 1542083
  8. Beyer WF, Fridovich I. Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem. 1987;161(2):559-566. https://doi.org/10.1016/ 0003-2697(87)90489-1 · DOI
  9. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1989;82(1):70-77. https://doi. org/10.1016/0003-9861(59)90090-6 References · DOI
  10. Zhang JP, Meng QY, Wang Q, Zhang LJ, Mao YL, Sun ZX. Effect of smoking on semen quality of infertile men in Shandong, China. Asian J Androl. 2000;2(2):143-146.
  11. Saleh, RA, Agarwal A, Sharma RK, Nelson DR and Thomas AJ. Effect of cigarette smoking on levels of seminal oxidative stress in infertile men: a prospective study. Fertil Steril. 2002;78(3):491-499. https://doi.org/10.1016/ S0015-0282(02)03294-6 · DOI
  12. Reddy S, Londonkar R, Reddy S, Patil SB. Testicular changes due to graded doses of nicotine in albino mice. Indian J Physiol Pharmacol. 1998;42(2):276-280.
  13. Guven MC, Can B, Ergün A, Saran Y, Aydos K. Ultrastructural effects of cigarette smoke on rat testis. Eur Urol. 1999;36(6):645-649. https:// doi.org/10.1159/000020061 · DOI
  14. Aydos K, Guven M, Can B, Ergun A. Nicotine toxicity to the ultrastructure of the testis in rats. BJU Int. 2001;88(6):622-626. https://doi.org/ 10.1046/j.1464-4096.2001.02384.x · DOI
  15. Gottfredsen RH, Larsen UG, Enghild JJ, Petersen SV. Hydrogen peroxide induce modifications of human extracellular superoxide dismutase that results in enzyme inhibition. Redox Biol. 2013;1(1):24-31. https://doi.org/10.1016/j.redox. 2012.12.004 · DOI
  16. Jain A, Flora S. Dose related effects of nicotine on oxidative injury in young, adult and old rats. J Environ Biol. 2012;33(2):233-238. (PMID: 23033686)
  17. Baskol G, Korkmaz S, Erdem F, Caniklioglu A, Kocyigit M, Aksu M. Assessment of nitric oxide, advanced oxidation protein products, malondialdehyde, and thiol levels in patients with restless legs syndrome. Sleep Med. 2012;13(4):414-418. https://doi.org/10.1016/j. sleep.2011.11.012 · DOI
  18. Fairuz A, Hashida N, Mahanem M. Effect of nicotine and goat milk co-administration on rat testis and sperm parameters. Austral J Basic Appl Sci 2011;5(12):2738-2741.
  19. Mahanem M, Nor-Asmaniza A, Phang H. Effects of nicotine and co-administration of nicotine and vitamin E on testis and sperm quality of adult rats. Malaysian Appl Biol. 2006;35(2):47-52.
  20. Sharma RK, Agarwal A. Role of reactive oxygen species in male infertility. Urol. 1996;48(6): 835-850. https://doi.org/10.1016/S0090-4295 (96)00313-5 · DOI
  21. Agarwal A, Said TM. Role of sperm chromatin abnormalities and DNA damage in male infertility. Human Repro Update. 2003;9(4):331-345. https://doi.org/10.1093/ humupd/dmg027 · DOI
  22. Taib IS, Budin SB, Ghazali AR, Jayusman PA, Louis SR, Mohamed J. Fenitrothion induced oxidative stress and morphological alterations of sperm and testes in male sprague-dawley rats. Clinics 2013;68(1):93-100. https://doi. org/10.606/clinics/2013(01)OA15
  23. WHO. Laboratory manual for the examination of human semen and sperm-cervical mucus interactions. 4th edition. Cambridge, United Kingdom: Cambridge University Press; 1999.
  24. Borgerding M, Klus H. Analysis of complex mixtures-cigarette smoke. Exp Toxicol Pathol. 2005;57(1):43-73. https://doi.org/10.1016/j.etp. 2005.05.010 · DOI
  25. Pacifici R, Altieri I, Gandini L, Lenzi A, Pichini S, Rosa M, et al. Nicotine, cotinine, and trans- 3-hydroxycotine levels in seminal plasma of smokers: effects on sperm parameters. Ther Drug Monit. 1993;15(5):358-363. https://doi. org/10.1097/00007691-199310000-00002 · DOI
  26. Jana K, Samanta PK, De DK. Nicotine diminishes testicular gametogenesis, steroidogenesis, and steroidogenic acute regulatory protein expression in adult albino rats: possible influence on pituitary gonadotropins and alteration of testicular antioxidant status. Toxicol Sci. 2010; 116(2):647-659. https://doi.org/10.1093/toxsci/ kfq149 · DOI
  27. Erat M, Ciftci M, Gumustekin K, Gul M. Effects of nicotine and vitamin E on glutathione reductase activity in some rat tissues in vivo and in vitro. Eur J Pharmacol. 2007;554(2-3):92-97. https://doi.org/10.1016/j.ejphar.2006.10.008 · DOI
  28. Arivazhagan P, Thilakavathy T, Panneerselvam C. Antioxidant lipoate and tissue antioxidants in aged rats. J Nutri Biochem. 2000;11(3):122-127. https://doi.org/10.1016/S0955-2863(99)00079-0 · DOI
  29. Griffith OW. Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal Biochem. 1980;106(1):207-212. https://doi.org/10.1016/ 0003-2697(80)90139-6 · DOI
  30. Gupta RK, Kesari AN, Diwakar S, Tyagi A, Tandon V, Chandra R, Watal G. In vivo evaluation of anti- oxidant and anti-lipidimic potential of Annona squamosa aqueous extract in Type 2 diabetic models. J Ethnopharmacol. 2008;118(1):21-25. https://doi.org/10.1016/j.jep.2008.03.008 · DOI

Cited by

Sorted by citations

Authors
School of Diagnostic and Applied Health Science, F. O. H. S., Budin, S. B., Jia Hui, K., School of Diagnostic and Applied Health Science, F. O. H. S., Jia Hui, L., School of Diagnostic and Applied Health Science, F. O. H. S., RamaLingam, A., School of Diagnostic and Applied Health Science, F. O. H. S., Jubaidi, F. F., School of Diagnostic and Applied Health Science, F. O. H. S., Latif, E. S., School of Diagnostic and Applied Health Science, F. O. H. S., Zainalabidin, S., School of Diagnostic and Applied Health Science, F. O. H. S., Taib, I. S., School of Diagnostic and Applied Health Science, F. O. H. S., Mohamed, J., & School of Diagnostic and Applied Health Science, F. O. H. S.
Affiliations
National University of Malaysia (MY)
ORCID
Venue
Malaysian Journal of Medical Sciences
Publisher
Universiti Sains Malaysia
Published
2017-12-29
Volume
24
Issue
6
Pages
50-57
ISSN
  • 1394-195X (ISSN-L)
  • 2180-4303
DOI
10.21315/mjms2017.24.6.6
PMC
PMC5771515
OpenAlex
W2783114551
Cited by
35
References
0
Citations by year
2026: 4 · 2025: 2 · 2024: 3 · 2023: 5 · 2022: 4 · 2021: 5 · 2020: 4 · 2019: 6 · 2018: 2
FWCI
3.67
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Malaysian Journal of Medical Sciences (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Universiti Kebangsaan Malaysia
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Health Sciences › Medicine › Physiology › Smoking Behavior and Cessation
Keywords
nicotine, oxidative stress, semen quality, male reproductive toxicity, testis, prostate, malondialdehyde, advanced oxidation protein products, superoxide dismutase, glutathione, testicular histopathology, nicotine replacement therapy

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review