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Most‐Probable‐Number Method for Microbial Populations
Abstract
The most-probable-number (MPN) method permits estimation of population density without an actual count of single cells or colonies. It is some-times called the method of ultimate or extinction dilution or, less descriptively, simply the dilution method. This chapter discusses the application of the MPN method for determining the total microbial count in soil. The MPN technique is based on a determination of the presence or absence of microorganisms in several individual aliquots of each of several consecutive dilutions of soil or other material. A prerequisite of the method is that the microorganism whose population is to be determined must be able to bring about some characteristic and readily recognizable transformation in the medium into which it is inoculated, or else the microorganism itself, after it has undergone multiplication, must be easily recognizable in the substrate.
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Cited by
Ying, G.-G., Yu, X.-Y., & Kookana, R. S. (2007) · Environmental Pollution · doi:10.1016/j.envpol.2007.02.013 · Cited by 357
Fortin, D., Davis, B., & Beveridge, T. J. (1996) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.1996.tb00329.x · Cited by 142
Mousavi, S. M., Motesharezadeh, B., Hosseini, H. M., Alikhani, H., & Zolfaghari, A. A. (2018) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2017.08.045 · Cited by 105
Lindsay, M. B. J., Wakeman, K. D., Rowe, O. F., Grail, B. M., Ptacek, C. J., Blowes, D. W., & Johnson, D. B. (2011) · Geomicrobiology Journal · doi:10.1080/01490451.2010.493570 · Cited by 56
Metadata
- Authors
- Alexander, M.
- Venue
- Agronomy Monographs
- Publisher
- Wiley
- Published
- 1965-01
- Pages
- 1467-1472
- ISSN
- 0065-4663 (ISSN-L)
- 2156-3276
- OpenAlex
- W2290266545
- Cited by
- 451
- References
- 0
- Type
- other
- Language
- en
- Open access
- closed
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- Alexander, M. (1965). Most‐Probable‐Number Method for Microbial Populations. Agronomy Monographs. https://doi.org/10.2134/agronmonogr9.2.c49
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