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Genotyping of Cre-lox Mice and Detection of Tissue-Specific Recombination by Multiplex PCR

Leneuve, P., Zaoui, R., Monget, P., Le Bouc, Y., Holzenberger, M.·2001·BioTechniques
DOI 10.2144/01315rr05
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Figure 1 Schematic representation of PCR-amplified loci. (A) Three primers (arrows) were designed to distinguish three related alleles of the IGF-IR gene: wild-type allele (W), floxed allele (Fl), and Crelox excised allele (X). LoxP sites are indicated by triangles. The sizes of the amplification products are given in bp. (B) A single pair of Cre primers amplified a 390-bp fragment from the wild-type recombinase coding region present in all Cre transgenics.
Figure 1 Schematic representation of PCR-amplified loci. (A) Three primers (arrows) were designed to distinguish three related alleles of the IGF-IR gene: wild-type allele (W), floxed allele (Fl), and Crelox excised allele (X). LoxP sites are indicated by triangles. The sizes of the amplification products are given in bp. (B) A single pair of Cre primers amplified a 390-bp fragment from the wild-type recombinase coding region present in all Cre transgenics.CC-BY · From the PDF
Image 2
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Image 3
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Figure 4 Comparison of PCR results and Southern analysis. (A) Representative examples of PCR-amplified (left) and Southern blot-analyzed (right) partial Cre recombination patterns from three mosaic mice (all EIIaCre/ flox) (6). PCR and Southern bands that represent the same alleles are connected by lines. (B) Comparison of a larger number of samples from EIIaCre/ flox, NestinCre/ flox, and InhibinCre /flox. Allele prevalence was determined from the relative band intensities measured from Southern blot autoradiographs and photographs taken from ethidium bromide-stained gels. Results obtained using PCR correlated strongly with Southern blot results from the same individuals ( n = 44).
Figure 4 Comparison of PCR results and Southern analysis. (A) Representative examples of PCR-amplified (left) and Southern blot-analyzed (right) partial Cre recombination patterns from three mosaic mice (all EIIaCre/ flox) (6). PCR and Southern bands that represent the same alleles are connected by lines. (B) Comparison of a larger number of samples from EIIaCre/ flox, NestinCre/ flox, and InhibinCre /flox. Allele prevalence was determined from the relative band intensities measured from Southern blot autoradiographs and photographs taken from ethidium bromide-stained gels. Results obtained using PCR correlated strongly with Southern blot results from the same individuals ( n = 44).CC-BY · From the PDF

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Research works printed but not linked: 1

  1. Lakso, M., J.G. Pichel, J.R. Gorman, B. Sauer, Y. Okamoto, E. Lee, F.W. Alt, and H. Westphal. 1996. Efficient in vivo manipula - tion of mouse genomic sequences at the zygote stage. Proc. Natl Acad. Sci. USA 93 :5860- 5865.

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Metadata

Authors
Leneuve, P., Zaoui, R., Monget, P., Le Bouc, Y., & Holzenberger, M.
Affiliations
Hôpital Saint-Antoine, Paris, 1INRA, Nouzilly, France
Venue
BioTechniques
Publisher
Informa UK Limited
Published
2001-11
Volume
31
Issue
5
Pages
1156-1162
ISSN
  • 0736-6205
  • 1940-9818
DOI
10.2144/01315rr05
Cited by
24
References
0
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (vor)

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CoChat. (2026). Citation check: Genotyping of Cre-lox Mice and Detection of Tissue-Specific Recombination by Multiplex PCR. https://app.cochat.ai/papers/10.2144/01315rr05
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Leneuve, P., Zaoui, R., Monget, P., Le Bouc, Y., & Holzenberger, M. (2001). Genotyping of Cre-lox Mice and Detection of Tissue-Specific Recombination by Multiplex PCR. BioTechniques. https://doi.org/10.2144/01315rr05