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Two-Stage PCR Protocol Allowing Introduction of Multiple Mutations, Deletions and Insertions Using QuikChange TM Site-Directed Mutagenesis
Open accessAbstract
We developed a two-stage procedure, based on the QuikChange Site-Directed Mutagenesis Protocol, that significantly expands its application to a variety of gene modification experiments. A pre-PCR, single-primer extension stage before the standard protocol allows the efficient introduction of not only point mutation but also multiple mutations and deletions and insertions to a sequence of interest.
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Cited by
Zhong, L., Li, B., Mah, C. S., Govindasamy, L., Agbandje-McKenna, M., Cooper, M., Herzog, R. W., Zolotukhin, I., Warrington, K. H., Weigel-Van Aken, K. A., et al. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0802866105 · Cited by 558
Alegre-Abarrategui, J., Christian, H., Lufino, M. M. P., Mutihac, R., Venda, L. L., Ansorge, O., & Wade-Martins, R. (2009) · Human Molecular Genetics · doi:10.1093/hmg/ddp346 · Cited by 432
Masuda, S., & Bauer, C. E. (2002) · Cell · doi:10.1016/s0092-8674(02)00876-0 · Cited by 391
Markusic, D. M., Herzog, R. W., Aslanidi, G. V., Hoffman, B. E., Li, B., Li, M., Jayandharan, G. R., Ling, C., Zolotukhin, I., Ma, W., et al. (2010) · Molecular Therapy · doi:10.1038/mt.2010.172 · Cited by 131
Schwartz, L. B., Min, H.-K., Ren, S., Xia, H.-Z., Hu, J., Zhao, W., Moxley, G., & Fukuoka, Y. (2003) · The Journal of Immunology · doi:10.4049/jimmunol.170.11.5667 · Cited by 120
Metadata
- Authors
- Wang, W., & Malcolm, B. A.
- Venue
- BioTechniques
- Publisher
- Informa UK Limited
- Published
- 1999-04
- Volume
- 26
- Issue
- 4
- Pages
- 680-682
- ISSN
- 0736-6205 (ISSN-L)
- 1940-9818
- OpenAlex
- W2418361432
- Cited by
- 586
- References
- 0
- FWCI
- 1.85
- Type
- journal article
- Language
- en
- Open access
- gold
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- CoChat. (2026). Citation check: Two-Stage PCR Protocol Allowing Introduction of Multiple Mutations, Deletions and Insertions Using QuikChange TM Site-Directed Mutagenesis. https://app.cochat.ai/papers/10.2144/99264st03
- The paper
- Wang, W., & Malcolm, B. A. (1999). Two-Stage PCR Protocol Allowing Introduction of Multiple Mutations, Deletions and Insertions Using QuikChange TM Site-Directed Mutagenesis. BioTechniques. https://doi.org/10.2144/99264st03
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
