Genomic DNA PCR analysis to assess xenograft development in mouse mammary gland
Open accessAbstract
The mouse transplantation model remains the most relevant methodology to assess the functional capacities of mammary cells and is particularly appropriate for investigations regarding mammary stem cells, whatever the species studied. Following xenotransplantation in mice mammary fat pad, the development of the xenograft is commonly evaluated by immunohistology. Here, we present a simple and rapid method to control the species specificity of a xenograft based on genomic DNA PCR amplification. DNA is extracted from the fixed samples intended for histology, thus allowing the reuse of precious samples. Standard and digital droplet PCR (requiring low DNA quantities) methods have been used to make the present method suitable for the analysis of xenotransplanted samples.
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dPCR provides exquisite sensitivity for absolute quantification of target nucleic acids.
“It requires low DNA quantities and provides exquisite sensitivity for absolute quantification of target nucleic acids [54,55,56].”
From Genetic Insights into the Economic Toll of Cell Line Misidentification: A Comprehensive Review (2026) · cites this paper as [56]
The Abstract says digital droplet PCR requires low DNA quantities and reports its use to detect bovine DNA in xenografts. That supports the low-DNA portion of the citing sentence, but the passages do not establish exquisite sensitivity or absolute quantification of target nucleic acids. (LLM, conf=0.78)
Experimental — accuracy not yet measureddPCR requires low DNA quantities.
“It requires low DNA quantities and provides exquisite sensitivity for absolute quantification of target nucleic acids [54,55,56].”
From Genetic Insights into the Economic Toll of Cell Line Misidentification: A Comprehensive Review (2026) · cites this paper as [56]
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Research works printed but not linked: 4
- DeOme KB , FaulkinLJJr, BernH, BlairPB. Development of mammary tumors from hyperplastic alveolar nodules transplanted into gland-free mammary fat pads of female C3H mice. Cancer Res.19(5), 515–525 (1959).
- Kuperwasser C , ChavarriaT, WuMet al. Reconstruction of functionally normal and malignant human breast tissues in mice. Proc. Natl Acad. Sci. USA101(14), 4966–4971 (2004).
- Proia DA , KuperwasserC. Reconstruction of human mammary tissues in a mouse model. Nat. Protoc.1(1), 206–214 (2006).
- Bockstaele L , BoulenouarS, VanDen Steen Get al. Evaluation of quantitative polymerase chain reaction markers for the detection of breast cancer cells in ovarian tissue stored for fertility preservation. Fertil. Steril.104(2), 410–417 (2015).
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Genetic Insights into the Economic Toll of Cell Line Misidentification: A Comprehensive Review
Weiskirchen, R. (2026) · Medical Sciences · doi:10.3390/medsci14010025 · Cited by 4 · see citing works
- Authors
- Aujean, E., Laubier, J., Brun, N., Finot, L., Chanat, E., Dessauge, F., Hue-Beauvais, C., & Provost, F. L.
- Affiliations
- AgroParisTech (FR)
- Institut Agro Rennes-Angers (FR)
- Université Paris-Saclay, INRAE,AgroParisTech, GABI, 78350, Jouy-en-Josas, France
- Venue
- BioTechniques
- Publisher
- Informa UK Limited
- Published
- 2020-01-28
- Volume
- 68
- Issue
- 4
- Pages
- 219-222
- ISSN
- 0736-6205 (ISSN-L)
- 1940-9818
- OpenAlex
- W3003311220
- Cited by
- 2
- References
- 0
- Citations by year
- 2026: 1 · 2024: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- BioTechniques (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- HAL (submittedVersion, cc-by-nc-nd)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- INRA Genetic Animal Division and Animal Physiology and Livestock Systems Division
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Health Sciences › Medicine › Surgery › Xenotransplantation and immune response
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Keywords
- mammary stem cells, species specificity, droplet digital PCR, sample reuse, mouse transplantation model
- MeSH terms
- Animals, Cattle, DNA, Female, Mammary Glands, Animal, Transplantation, Heterologous, Polymerase Chain Reaction, Genomics, Mice, Heterografts
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