The 24-hour skin hydration and barrier function effects of a hyaluronic 1%, glycerin 5%, and Centella asiatica stem cells extract moisturizing fluid: an intra-subject, randomized, assessor-blinded study
Open accessAbstract
Introduction: Moisturizing products are commonly used to improve hydration in skin dryness conditions. However, some topical hydrating products could have negative effects on skin barrier function. In addition, hydrating effects of moisturizers are not commonly evaluated up to 24 hours after a single application. Hyaluronic acid (HA) and glycerin are very well-known substances able to improve skin hydration. Centella asiatica extract (CAE) could exert lenitive, anti-inflammatory and reepithelialization actions. Furthermore, CAE could inhibit hyaluronidase enzyme activity, therefore prolonging the effect of HA. A fluid containing HA 1%, glycerin 5% and stem cells CAE has been recently developed (Jaluronius CS [JCS] fluid). Study aim: To evaluate and compare the 24-hour effects of JCS fluid on skin hydration and on transepidermal water loss (TEWL) in healthy subjects in comparison with the control site. Subjects and methods: Twenty healthy women, mean age 40 years, were enrolled in an intra-subject (right vs left), randomized, assessor-blinded, controlled, 1-day trial. The primary end points were the skin hydration and TEWL, evaluated at the volar surface of the forearm and in standardized conditions (temperature- and humidity-controlled room: 23°C and 30% of humidity) using a corneometer and a vapometer device at baseline, 1, 8 and 24 hours after JCS fluid application. Measurements were performed by an operator blinded for the treatments. Results: Skin hydration after 24 hours was significantly higher ( P =0.001; Mann–Whitney U test) in the JCS-treated area in comparison with the control site. JCS induced a significant ( P =0.0001) increase in skin hydration at each evaluation time (+59% after 1 hour, +48% after 8 hours and +29% after 24 hours) in comparison with both baseline ( P =0.0001) and non-treated control site ( P =0.001). TEWL after 24 hours was significantly lower ( P =0.049; Mann–Whitney U test) in the JCS-treated area in comparison with the control site (13±4 arbitrary units [AU] vs 16±6 AU). JCS fluid significantly reduced post-stripping TEWL in comparison with baseline after 1, 8 and 24 hours (–52%, –32% and –48%, respectively). In the control site, TEWL was not reduced in comparison with baseline values at each time point’s evaluation. Conclusion: A single application of JCS significantly improves skin hydration for up to 24 hours at the same time as improving skin barrier function. Keywords: moisturizing, hyaluronic acid, Centella asiatica stem cells, randomized controlled trial
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A randomized assessor-blinded clinical study reported improved skin hydration and reduced TEWL after topical use of a formulation containing C. asiatica-derived actives.
“In line with these cellular findings, a randomized assessor-blinded clinical study reported improved skin hydration and reduced TEWL after topical use of a formulation containing C. asiatica–derived actives [29].”
Existing human intervention studies of oral Centella asiatica have often been constrained by small sample sizes, short intervention periods, or subjective outcome measures.
“While Centella asiatica has been extensively studied in topical formulations, randomized controlled trials evaluating oral supplementation remain limited, and existing human intervention studies have often been constrained by small sample sizes, short intervention periods, or subjective outcome measures [25,30,31,32].”
From A Randomized, Double-Blind, Placebo-Controlled Trial Assessing the Effects of Oral Centella asiatica Extract on Skin Aging-Related Parameters in Middle-Aged Korean Women. (2026) · cites this paper as [31]
The Subjects and methods > Population section describes a one-day study of a topically applied moisturizing fluid in 20 women, with measured skin hydration and transepidermal water loss as outcomes. Searches for oral supplementation and limitations of oral intervention studies found no passage addressing those claims. No page numbers were provided in our copy. (LLM, conf=0.76)
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References 31 · Cited by 53
- Authors
- Milani, M., & Sparavigna, A.
- Affiliations
- Difa Cooper (Italy) (IT)
- Corresponding author
- Milani, Massimo
- Venue
- Clinical, Cosmetic and Investigational Dermatology
- Publisher
- Informa UK Limited
- Published
- 2017-08
- Volume
- Volume 10
- Pages
- 311-315
- ISSN
- 1178-7015 (ISSN-L)
- PMC
- PMC5560567
- OpenAlex
- W2744828104
- Cited by
- 53
- References
- 31
- Citations by year
- 2026: 5 · 2025: 12 · 2024: 8 · 2023: 7 · 2022: 5 · 2021: 5 · 2020: 6 · 2019: 2 · 2018: 2 · 2017: 1
- FWCI
- 3.42
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Clinical Cosmetic and Investigational Dermatology (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Dove Medical Press (Taylor and Francis Group) (submittedVersion, cc-by-nc)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
- Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
- Keywords
- skin hydration, skin barrier function, transepidermal water loss, hyaluronic acid, glycerol, hyaluronidase inhibition, healthy skin, anti-aging cosmetics, skin aging, dry skin
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