CoChat
Sign Up
Cited by 1Wound Healing and TreatmentsMedicinal Plant ResearchPhytochemistry and biological activity of medicinal plants

In-vivo Wound Healing and Anti-Inflammatory Activity of the Solvent Fraction of Zehneria scabra L.F. Sond (Cucurbitaceae) Leaves

Open access
Fisseha, Nebeyi, Hammeso, Workineh Woldeselassie, Nureye, Dejen, Tesfaye, Tarekegn, Yimer, Tesfaye·2022·Journal of Experimental Pharmacology
DOI 10.2147/jep.s387364PMC PMC9725919
Open this paper in CoChat — free accountOpen PDF

Abstract

Background: Wounds continue to be a difficult clinical problem, with early and late consequences causing significant morbidity and death. As a result, proper wound management is critical. In addition to contemporary medicine, medicinal herbs serve an essential role in the treatment of wounds and bacterial infections. Z. scabra is a medicinal plant that has traditionally been used to treat wounds. However, there are no scientific reports on solvent fraction wound healing activities. As a result, the current study presents a scientific assessment of the wound healing ability of the solvent fractions of Z. scabra leaves. Methods: The leaves were crushed and macerated three times in 80% methanol. Chloroform, ethyl acetate, and aqueous fractions of simple ointment at 5% w/w and 10 percent w/w strengths were prepared using the fusion technique based on the British Pharmacopoeia. Excision and incision wound models were used to assess the solvent fractions’ wound healing activities. The anti-inflammatory efficacy of crude and solvent fractions was tested in mice utilizing a carrageenan-induced hindpaw edema model. Results: In rats, a test dose of 2000 mg/kg of the 10% w/w crude extract ointment was found to be safe. Groups treated with the 5% and 10% ethyl acetate fractions of the extract experienced significant (p< 0.05 and p< 0.01) wound reduction in the excision wound model. When compared to the negative control, the length of epithelization in groups treated with 10% ethyl acetate fraction and aqueous fractions of Z. scabra was statistically significant (p 0.001). By lowering the amount of carrageenan-induced paw edema, the leaf extract and the chloroform fraction of Z. scabra demonstrated a dose-dependent anti-inflammatory effect. Conclusion: The extract showed remarkable wound healing and anti-inflammatory activity and might be recommended for the treatment of many types of human wounds. Keywords: wound healing, anti-inflammatory excision, wound model, Zehneria scabra

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.2147/jep.s387364

Cites

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

No references could be resolved yet.

Research works printed but not linked: 35

  1. [1]Masson‐Meyers DS, Andrade TA, Caetano GF, et al. Experimental models and methods for cutaneous wound healing assessment. Int J Exp Pathol. 2020; 101(1–2): 21– 37. doi: 10.1111/iep.12346 32227524 PMC7306904 · DOI
  2. [2]Nguyen DT, Orgill DP, Murphy GF. The pathophysiologic basis for wound healing and cutaneous regeneration. In: Biomaterials for Treating Skin Loss. Woodhead Publishing; 2009: 25– 57.
  3. [3]Nahla Saeed IA Evaluation of gastro protective effect and wound healing potential of cibotium barometz and vitex pubecsens in an animal model/Nahla Saeed Ibraheem Alwajeeh Doctoral dissertation. University of Malaya; 2017.
  4. [4]Muluye AB, Desta AG, Abate SK, et al. Anti-malarial activity of the root extract of Euphorbia abyssinica (Euphorbiaceae) against Plasmodium berghei infection in mice. Malar J. 2019; 18: 261. doi: 10.1186/s12936-019-2887-7 31362744 PMC6668069 · DOI
  5. [5]Atkin L. Understanding methods of wound debridement. Br J Nurs. 2014; 23(12): S10– S5. doi: 10.12968/bjon.2014.23.sup12.S10 25075385 · DOI
  6. [6]García-Villén F, Faccendini A, Aguzzi C, et al. Montmorillonit- norfloxacin nanocomposite intended for healing of infected wounds. Int J Nanomedicine. 2019; 14: 5051– 5060. doi: 10.2147/IJN.S208713 31371946 PMC6628958 · DOI
  7. [7]Hamdan S, Pastar I, Drakulich S, et al. Nanotechnology-driven therapeutic interventions in wound healing: potential uses and applications. ACS Cent Sci. 2017; 3(3): 163– 175. doi: 10.1021/acscentsci.6b00371 28386594 PMC5364456 · DOI
  8. [8]Agyare C, Boakye YD, Bekoe EO, Hensel A, Dapaah SO, Appiah T. African medicinal plants with wound healing properties. J Ethnopharmacol. 2016; 11(177): 85– 100. doi: 10.1016/j.jep.2015.11.008 26549271 · DOI
  9. [9]Gedif T, Hahn HJ. The use of medicinal plants in self-care in rural central Ethiopia. J Ethnopharmacol. 2003; 87(2–3): 155– 161. doi: 10.1016/S0378-8741(03)00109-0 12860301 · DOI
  10. [10]Teklay A, Abera B, Giday M. An ethnobotanical study of medicinal plants used in kilte awulaelo district, tigray region of Ethiopia. J Ethnobiol Ethnomed. 2013; 9(1): 1– 23. doi: 10.1186/1746-4269-9-65 24011232 PMC3852788 · DOI
  11. [11]Tekle Y. An ethno-veterinary botanical survey of medicinal plants in Kochore district of Gedeo zone, southern nations nationalities and peoples regional state (SNNPRs), Ethiopia. J Sci Innov Res. 2014; 3(4): 433– 445. doi: 10.31254/jsir.2014.3407 · DOI
  12. [12]Kefalew A, Asfaw Z, Kelbessa E. Ethnobotany of medicinal plants in Ada’a District, East Shewa Zone of Oromia regional state, Ethiopia. J Ethnobiol Ethnomed. 2015; 11(1): 1– 28. doi: 10.1186/s13002-015-0014-6 25889311 PMC4419563 · DOI
  13. [13]Abew B, Sahile S, Moges F. In vitro antibacterial activity of leaf extracts of Z. scabra and Ricinus communis against Escherichia coli and methicillin resistance Staphylococcus aureus. Asian Pac J Trop Biomed. 2014; 4(10): 816– 820. doi: 10.12980/APJTB.4.201414B16 · DOI
  14. [14]Agogbua J, Ekeke C, Okoli BE. Morpho-anatomical characters of Zehneria capillacea (Schumach) C. Jeffrey and Z. scabra (LF) Sond Cucurbitaceae. Afr J Plant Sci. 2015; 9(12): 457– 465. doi: 10.5897/AJPS2015.1306 · DOI
  15. [15]Murthy KS, Ravindranath D, Sandhya RS, Pullaiah T. Ethnobotany and distribution of wild and cultivated genetic resources of Cucurbitaceae in the Eastern Ghats of Peninsular India. Top Class J Herbal Med. 2013; 2(6): 149– 158.
  16. [17]Nureye D, Tekalign E, Fisseha N, Tesfaye T, Hammeso WW. Evaluation of antiplasmodial activity of hydroalcoholic crude extract and solvent fractions of Z. scabra roots against plasmodium berghei in Swiss albino mice. Infect Drug Resist. 2021; 14: 2583. doi: 10.2147/IDR.S314262 34262303 PMC8275019 · DOI
  17. [18]Council NR. Guide for the Care and Use of Laboratory Animals. Washington, DC, USA: National Academies Press; 2010.
  18. [19]Tekleyes B, Huluka SA, Wondu K, Wondmkun YT. Wound healing activity of 80 percent methanol leaf extract of Z. scabra (Lf) sond (cucurbitaceae) in mice. J Exp Pharmacol. 2021; 13: 537. doi: 10.2147/JEP.S303808 34104005 PMC8178697 · DOI
  19. [21]Geetha TS, Geetha N. Phytochemical screening, quantitative analysis of primary and secondary metabolites of Cymbopogan citratus (DC) Stapf. Leaves from Kodaikanal Hills, Tamilnadu. Int J Pharmtech Res. 2014; 6(2): 521– 529.
  20. [22]Wadood A, Ghufran M, Jamal SB, Naeem M, Khan A, Ghaffar R. Phytochemical analysis of medicinal plants occurring in local area of Mardan. Biochem Anal Biochem. 2013; 2(4): 1– 4. doi: 10.4172/2161-1009.1000144 · DOI
  21. [23]Mulisa E, Asres K, Engidawork E. Evaluation of wound healing and anti-inflammatory activity of the rhizomes of Rumex abyssinicus J. (Polygonaceae) in mice. BMC Complement Altern Med. 2015; 15(1): 341. doi: 10.1186/s12906-015-0878-y 26423525 PMC4589968 · DOI
  22. [24]Belachew TF, Asrade S, Geta M, Fentahun E. In vivo evaluation of wound healing and anti-inflammatory activity of 80% methanol crude flower extract of hagenia abyssinica (Bruce) J.F. Gmel in Mice. Evid-Based Complement Altern Med. 2020; 2020: 1– 12. doi: 10.1155/2020/9645792 PMC7149342 32308725 · DOI
  23. [25]Lambebo MK, Kifle ZD, Gurji TB, Yesuf JS. Evaluation of wound healing activity of methanolic crude extract and solvent fractions of the leaves of vernonia auriculifera hiern (asteraceae) in mice. J Exp Pharmacol. 2021; 13: 677. doi: 10.2147/JEP.S308303 34326671 PMC8315810 · DOI
  24. [26]Ambikar D, Tsegaw A, Belayneh YM, Belayneh YM. Wound healing activity of 80 percent methanolic crude extract and solvent fractions of the leaves of justicia schimperiana (Hochst. ex Nees) T. Anderson (Acanthaceae) in Mice. J Exp Pharmacol. 2022; 14: 167. doi: 10.2147/JEP.S340177 35592645 PMC9113456 · DOI
  25. [27]Upadhyay A, Chattopadhyay P, Goyary D, Mitra Mazumder P, Veer V. Ixora coccinea enhances cutaneous wound healing by upregulating the expression of collagen and basic fibroblast growth factor. ISRN Pharmacol. 2014; 2014: w356. doi: 10.1155/2014/751824 PMC3928818 24624303 · DOI
  26. [28]Mekonnen A, Sidamo T, Asres K, Engidawork E. In vivo wound healing activity and phytochemical screening of the crude extract and various fractions of Kalanchoe petitiana A. Rich (Crassulaceae) leaves in mice. J Ethnopharmacol. 2013; 145(2): 638– 646. doi: 10.1016/j.jep.2012.12.002 23228912 · DOI
  27. [29]Assefa A, Urga K, Melaku D, Guta M, Mekonnen W. Bronchodilator and anti-inflammatory activities of Adhatoda schimperiana. Ethiop J Health Sci. 2008; 18: 2.
  28. [30]Robert JN, Els N, Danny EC, Petra GB, Klaske N, Paul L. Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr. 2001; 74: 418– 425. doi: 10.1093/ajcn/74.4.418 11566638 · DOI
  29. [31]Mekonnen NA, Atnafie AS, Atta WM. Evaluation of the anti-inflammatory activities of 70 percent ethanol leaves extract and solvent fractions of Z. scabra (Lf) Sond (Cucurbitaceae) in rodents. Evid-Based Complement Altern Med. 2020; 2020: 11.
  30. [32]Umer S, Asres K, Veeresham C. Hepatoprotective activities of two Ethiopian medicinal plants. Pharm Biol. 2010; 48(4): 461– 468. doi: 10.3109/13880200903173593 20645727 · DOI
  31. [33]Sagbo IJ, Afolayan AJ, Bradley G. Antioxidant, antibacterial and phytochemical properties of two medicinal plants against the wound infecting bacteria. Asian Pac J Trop Biomed. 2017; 7(9): 817– 825. doi: 10.1016/j.apjtb.2017.08.009 · DOI · Paper page
  32. [34]Ali A, Alam S, Ahmad S, Ali S, Ahsan M, Siddiqui W. Wound healing activity of alcoholic extract of Tamarix aphylla L. on animal models. Biomed Pharmacol J. 2019; 12(1): 41– 48. doi: 10.13005/bpj/1611 · DOI
  33. [35]Arun M, Satish S, Anima P. Evaluation of wound healing, antioxidant and antimicrobial efficacy of Jasminum auriculatum Vahl. leaves. Avicenna J Phytome. 2016; 6(3): 295. PMC4930536 27462552
  34. [36]Samanta R, Pattnaik AK, Pradhan KK, Mehta BK, Pattanayak SP, Banerjee S. Wound healing activity of silibinin in mice. Pharmacognosy Res. 2016; 8(4): 298. doi: 10.4103/0974-8490.188880 27695272 PMC5004523 · DOI
  35. [37]Samejo MQ, Sumbul A, Shah S, Memon SB, Chundrigar S. Phytochemical screening of Tamarix dioica Roxb. ex Roch. J Pharm Res. 2013; 7(2): 181– 183. doi: 10.1016/j.jopr.2013.02.017 · DOI

Cited by

Sorted by citations

Authors
Fisseha, N., Hammeso, W. W., Nureye, D., Tesfaye, T., & Yimer, T.
Affiliations
  • Mizan Tepi University (ET)
  • Dilla University (ET)
  • Debre Tabor University (ET)
Corresponding author
Fisseha, Nebeyi
ORCID
Venue
Journal of Experimental Pharmacology
Publisher
Informa UK Limited
Published
2022-12
Volume
Volume 14
Pages
367-377
ISSN
1179-1454 (ISSN-L)
DOI
10.2147/jep.s387364
PMC
PMC9725919
OpenAlex
W4311020547
Cited by
1
References
0
Citations by year
2024: 1
FWCI
0.11
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Experimental Pharmacology (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Dove Medical Press (Taylor and Francis Group) (submittedVersion, cc-by-nc)
Topics
  • Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and biological activity of medicinal plants
Keywords
Zehneria scabra, wound healing, anti-inflammatory activity, solvent fractions, ethyl acetate fraction, chloroform fraction, aqueous fraction, ointment formulation, excision wound, incision wound, re-epithelialization, carrageenan-induced paw edema, medicinal plants, Cucurbitaceae

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review