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Effects on the Post-translational Modification of H3K4Me3, H3K9ac, H3K9Me2, H3K27Me3, and H3K36Me2 Levels in Cerebral Cortex, Hypothalamus and Pons of Rats after a Systemic Administration of Cannabidiol: A Preliminary Study
Abstract
Background: Cannabidiol (CBD), a non-psychotropic constituent of Cannabis sativa, has shown therapeutic promises by modulating several pathological conditions, including pain, epilepsy autism, among others. However, the molecular mechanism of action of CBD remains unknown and recent data suggest the engagement on CBD´s effects of nuclear elements, such as histone activity. Aim: This study assessed the changes in the post-translational modification (PTM) on the histones H3K4Me3, H3K9ac, H3K9Me2, H3K27Me3, and H3K36Me2 in several brain regions of rats after the administration of CBD (20 mg/Kg/i.p.). Objective: To evaluate the effects on the PTM of histones H3K4Me3, H3K9ac, H3K9Me2, H3K27Me3, and H3K36Me2 levels in the cerebral cortex, hypothalamus and pons of CBD-treated rats. Methods: Ten adult rats were randomly assigned into 2 groups: Control or CBD (20 mg/Kg/i.p). Animals were sacrificed after treatments and brains were collected for dissections of the cerebral cortex, hypothalamus and pons. Samples were analyzed for PTM on the histones H3K4Me3, H3K9ac, H3K9Me2, H3K27Me3, and H3K36Me2 levels by Western blot procedure. Results: CBD increased the PTM levels on the histones H3K4Me3, H3K9ac, and H3K27Me3 in the cerebral cortex whereas no significant differences were found in H3K9Me2 and H3K36Me2. In addition, in the hypothalamus, CBD decreased the contents of H3K9ac while no significant effects were observed in H3K4Me3, H3K9Me2, H3K27Me3, and H3K36Me2. Lastly, in the pons, CBD- treated rats showed a significant decline on the PTM levels of H3K4Me3 whereas no statistical differences were found in H3K9ac, H3K9Me2, H3K27Me3, and H3K36Me2. Conclusion: The study showed that CBD induced differential effects in levels of PTM on the histones H3K4Me3, H3K9ac, H3K9Me2, H3K27Me3, and H3K36Me2 in several brain regions.
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Several histone modifications were changed in some brain regions of rats after CBD administration.
“A preliminary study reported that several histone modifications were changed in some brain regions of rats after CBD administration.398”
From Epigenetic regulation in major depression and other stress-related disorders: molecular mechanisms, clinical relevance and therapeutic potential (2023) · cites this paper as [398]
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Yuan, M., Yang, B., Rothschild, G., Mann, J. J., Sanford, L. D., Tang, X., Huang, C., Wang, C., & Zhang, W. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01519-z · Cited by 246
“398 But the molecular mechanism of CBD action remains to be investigated.”
Background
Metadata
- Authors
- Pastrana-Trejo, J. C., Duarte-Aké, F., Us-Camas, R., De-la-Peña, C., Parker, L., Pertwee, R. G., & Murillo-Rodríguez, E.
- Affiliations
- Anahuac Mayab University (MX)
- Centro de Investigación Científica de Yucatán, A.C (MX)
- Centro de Investigacion Científica de Yucatan, A.C. Unidad de Biotecnologia, Merida, Yucatan, Mexico
- University of Guelph (CA)
- University of Aberdeen (GB)
- Intercontinental Neuroscience Research Group, Laboratorio de Neurociencias Moleculares e Integrativas, Escuela de Medicina, Division Ciencias de la Salud, Universidad Anahuac Mayab Merida, Yucatan, Mexico
- ORCID
- Venue
- Central Nervous System Agents in Medicinal Chemistry
- Publisher
- Bentham Science Publishers Ltd.
- Published
- 2021-08
- Volume
- 21
- Issue
- 2
- Pages
- 142-147
- ISSN
- 1568-0150 (ISSN-L)
- 1871-5249
- OpenAlex
- W3087788727
- Cited by
- 20
- References
- 0
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 8 · 2023: 2 · 2022: 3 · 2021: 1
- FWCI
- 0.90
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Central Nervous System Agents in Medicinal Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Consejo Nacional de Ciencia y Tecnología (CN-17-19)
- Topics
- Health Sciences › Medicine › Pharmacology (medical) › Cannabis and Cannabinoid Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- cannabidiol, histone modifications, H3K4me3, H3K9ac, H3K9me2, H3K27me3, H3K36me2, histone acetylation, histone methylation, cerebral cortex, hypothalamus, passive optical networks, brain regions, rats, epigenetics
- MeSH terms
- Animals, Cannabidiol, Cerebral Cortex, Histones, Hypothalamus, Pons, Protein Processing, Post-Translational, Rats
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