Molecular Typing of mcr-1 Escherichia Coli Isolates From Pigs and Farm Environment Based on fumC and fimH Alleles
Abstract
Background: The dissemination of polymyxin resistance represents a significant threat to public health. Materials & methods: Sequence-based typing was performed by 53 mcr-1 Escherichia coli isolates using fumC/fimH (CH) genes to characterize clones spreading from pig farming. Furthermore, 12 isolates had their whole genome sequenced for phylogenetic study. Results: The isolates were classified into 22 distinct CH types, and two novel CH types (CH41-1578 and CH4-1579) and one sequence type (ST12652) was also described. According to phylogenetic study, both multilocus sequence typing and CH methods grouped the isolates similarly. Conclusion: Our findings suggest that the dissemination of the mcr-1 gene in pig farming has occurred mainly by horizontal gene transfer, and CH typing proved to be a good tool to characterize E. coli clones.
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Phylogenetic placement analysis on genes fumC and fimH confirmed that E. coli 5C belongs to the E. coli species.
“Phylogenetic placement analysis performed on genes fumC and fimH, both in strain 5C and in strain Nissle 1917, used as control (Online Resource 4), confirmed that the strain E. coli 5C belongs to the E. coli species [114].”
From Selection, Comparative Genomics, and Potential Probiotic Features of Escherichia coli 5C, a pks-Negative Strain Isolated from Healthy Infant Donor Feces (2026) · cites this paper as [114]
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References 49 · Cited by 3
- Authors
- Oliveira, G. S., Lentz, S. A., Wink, P. L., & Martins, A. F.
- Affiliations
- Universidade Federal do Rio Grande do Sul (BR)
- Programa de Pós-graduação em Microbiologia Agrícola e do Ambiente, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, 90050-170, Brazil
- Hospital de Clínicas de Porto Alegre (BR)
- Venue
- Future Microbiology
- Publisher
- Informa UK Limited
- Published
- 2023-05
- Volume
- 18
- Issue
- 8
- Pages
- 481-488
- ISSN
- 1746-0913 (ISSN-L)
- 1746-0921
- OpenAlex
- W4378782645
- Cited by
- 3
- References
- 49
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1
- FWCI
- 0.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Future Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Antimicrobial ((MCTI/CNPq/CAPES/FAPs no. 16/2014))
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Life Sciences › Agricultural and Biological Sciences › Food Science › Salmonella and Campylobacter epidemiology
- Keywords
- mcr-1, Escherichia coli, polymyxin resistance, molecular typing, pigs, farm environment, horizontal gene transfer, phylogenetic analysis, novel sequence types, antimicrobial resistance dissemination
- MeSH terms
- Farms, Alleles, Animals, Anti-Bacterial Agents, Colistin, Escherichia coli, Escherichia coli Infections, Microbial Sensitivity Tests, Phylogeny, Swine, Adhesins, Escherichia coli, Escherichia coli Proteins, Fimbriae Proteins, Multilocus Sequence Typing
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