High cell density cultivation of Bacillus subtilis NCIM 2063: Modeling, optimization and a scale-up procedure
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Bacillus subtilis is a non-pathogenic, sporulating, Gram-positive bacteria with pronounced antimicrobial and metabolic activity and great potential for wide application in various fields. The aim of this paper was to determine the optimum B. subtilis NCIM 2063 growth conditions and to scale up biomass production from shake flasks to a bioreactor level. The critical growth parameters and their interaction effects were studied using Box?Behnken experimental design and response surface methodology. Developed model equations were statistically significant with good prediction capability. It was found that during shake flask cultivation glucose should be added in concentration up to 5 g L-1 in DSM medium, OTR at 10 mol m-3 h-1 and temperature of 33?C, to achieve the maximum number of viable cells and spores. To scale up the process from shake flasks to the bioreactor level kLa was used as a main criterion. Scale up effect was evaluated by comparing the growth kinetics in the shake flasks and in a laboratory bioreactor. The total number of cells obtained in the bioreactor was 4.57x109 CFU mL-1 which is 1.41 times higher than the number of cells in the shake flasks (3.24x109 CFU mL-1), proving that the scale-up procedure was conducted successfully.
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Previous studies suggest that optimal temperature and agitation ranges for B. subtilis strains are 30°C-37°C and 200-250 rpm, respectively.
“Previous studies on B. subtilis strains suggest that their optimal temperature and agitation ranges are 30°C-37°C and 200-250 rpm, respectively [34, 39 ,40].”
From Optimization of Bacillus subtilis PW12 biomass production using RSM: a preliminary study towards single-cell protein production for aquaculture (2024) · cites this paper as [34]
Glucose is a preferred carbon source for B. subtilis and other Bacilli.
“Previous studies also show that glucose is a preferred carbon source for B. subtilis and other Bacilli [32-34].”
From Optimization of Bacillus subtilis PW12 biomass production using RSM: a preliminary study towards single-cell protein production for aquaculture (2024) · cites this paper as [34]
Cites
A Procedure for High-Yield Spore Production by Bacillus s ubtilis
Monteiro, S. M., Clemente, J. J., Henriques, A. O., Gomes, R. J., Carrondo, M. J., & Cunha, A. E. (2008) · Biotechnology Progress · doi:10.1021/bp050062z · Cited by 119 · see citing works
Bacillus Based Microbial Formulations: Optimization of the Production Process
Stamenkovic-Stojanovic, S., Karabegovic, I., Beskoski, V., Nikolic, N., & Lazic, M. (2019) · Hemijska industrija · doi:10.2298/hemind190214014s · Cited by 37 · see citing works
Khardziani, T., Kachlishvili, E., Sokhadze, K., Elisashvili, V., Weeks, R., Chikindas, M. L., & Chistyakov, V. (2017) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-017-9303-9 · Cited by 35 · see citing works
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Gracious, M., & Nandakumar, S. (2024) · Journal of Applied Biology & Biotechnology · doi:10.7324/jabb.2024.177031 · Cited by 2 · see citing works
“Previous studies on B. subtilis strains suggest that their optimal temperature and agitation ranges are 30°C-37°C and 200–250 rpm, respectively [34, 39 ,40].”
- Authors
- Stamenkovic-Stojanovic, S., Karabegovic, I., Danilovic, B., Mancic, S., & Lazic, M.
- Affiliations
- Faculty of Technology, University of Niš, Leskovac, Serbia
- Venue
- Journal of the Serbian Chemical Society
- Publisher
- National Library of Serbia
- Published
- 2023
- Volume
- 88
- Issue
- 11
- Pages
- 1103-1117
- ISSN
- 0352-5139 (ISSN-L)
- 1820-7421
- OpenAlex
- W4384135155
- Cited by
- 5
- References
- 3
- Citations by year
- 2026: 5
- FWCI
- 0.40
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Journal of the Serbian Chemical Society (publishedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Ministry of Education, Science and Technological Development of the Republic of Serbia (451-03-47/2023-01/200133)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Fluid Dynamics and Mixing
- Life Sciences › Agricultural and Biological Sciences › Food Science › Advanced Scientific Research Methods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Bacillus subtilis, high cell density culture, biomass production, scale-up, bioreactors, shake flask, glucose concentration, oxygen transfer rate, temperature, growth kinetics, viable cell count, sporulation, oxygen transfer coefficient
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