Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle
Open accessAbstract
A single bout of aerobic exercise can enhance insulin action, but whether a similar effect occurs after resistance exercise is unknown. Hyperinsulinemic-euglycemic clamps were performed on eight male subjects at rest and after a single bout and three repeated bouts of resistance exercise over 7 days. Skeletal muscle biopsies were taken before and after the clamp and immediately after a single exercise bout. Whole-body insulin action measured by glucose infusion rate decreased (P < 0.05) after a single exercise bout, whereas in response to repeated bouts of resistance exercise, the glucose infusion rate was similar to the rest trial. In skeletal muscle, Akt substrate of 160 kDa (AS160) phosphorylation, an Akt substrate implicated in the regulation of GLUT4 translocation, and its interaction with 14-3-3 was decreased (P < 0.05) only after a single exercise bout. Insulin increased (P < 0.05) phosphorylation of AS160 and its interaction with 14-3-3, but the insulin response was not influenced by resistance exercise. Phosphorylation of insulin receptor substrate-1 and Akt were similar to changes in AS160 phosphorylation after exercise and/or insulin. In conclusion, a single bout of resistance exercise impairs whole-body insulin action. Regulation of AS160 and interaction with 14-3-3 in skeletal muscle are influenced by resistance exercise and insulin but do not fully explain the effect of resistance exercise on whole-body insulin action.
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Exercise-induced AMPK is thought to phosphorylate and inhibit the Rab-GAP proteins TBC1D1/TBC1D4.
“In addition to this event exercise induced AMPK is also thought to phosphorylate and inhibit the Rab-GAP proteins TBC1D1/TBC1D4 [16][17][18].”
From New strategies in sport nutrition to increase exercise performance (2016) · cites this paper as [18]
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References 39 · Cited by 37
- Authors
- Howlett, K. F., Sakamoto, K., Garnham, A., Cameron-Smith, D., & Hargreaves, M.
- Affiliations
- Deakin University (AU)
- University of Dundee (GB)
- School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria, Australia
- The University of Melbourne (AU)
- Venue
- Diabetes
- Publisher
- American Diabetes Association
- Published
- 2007-06-01
- Volume
- 56
- Issue
- 6
- Pages
- 1608-1614
- ISSN
- 0012-1797 (ISSN-L)
- 1939-327X
- OpenAlex
- W2126014959
- Cited by
- 37
- References
- 39
- Citations by year
- 2023: 2 · 2022: 2 · 2020: 2 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 3 · 2014: 4 · 2013: 2 · 2012: 3
- FWCI
- 1.99
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Diabetes (publishedVersion)
- PubMed (publishedVersion)
- Victoria University Research Repository (Victoria University) (submittedVersion)
- Funders
- Diabetes Australia Research Trust
- Medical Research Council
- Australian Research Council (DP0450338)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Keywords
- resistance training, insulin action, AS160, skeletal muscle, GLUT4 translocation, IRS-1, Akt, exercise training, hyperinsulinemic-euglycemic clamp, glucose infusion rate
- MeSH terms
- Adenylate Kinase, Adult, Aerobiosis, Physical Exertion, Humans, Hyperinsulinism, Insulin, Male, Oxygen Consumption, Reference Values, Glucose Clamp Technique, Exercise, Muscle, Skeletal, GTPase-Activating Proteins, 14-3-3 Proteins
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