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Cited by 67Diabetes Management and ResearchPancreatic function and diabetesDiabetes, Cardiovascular Risks, and Lipoproteins

Application of Isotopic Techniques Using Constant Specific Activity or Enrichment to the Study of Carbohydrate Metabolism

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Vella, Adrian, Rizza, Robert A.·2009·Diabetes
DOI 10.2337/db09-0318PMC PMC2750215
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Abstract

Tracer techniques have become an establishedpart of metabolic research and indeed are afundamental part of our understanding of carbo-hydrate metabolism in humans. This review is meant to provide a basic overview of the problems and potential pitfalls associated with these techniques in the hope that they are reliably applied to newer areas of research. A quantitative measurement of the substrate fluxes that determine fasting or postprandial glucose concentrations is central to the determination of the pathophysiology of disease states and of measuring the effect of therapeutic interventions on the disease state. During the fasting state, glucose concentrations are dependent on the rate of endogenous glucose production (EGP) relative to the rate of glucose disappearance; measurement using tracer tech-niques is quite straightforward. The situation is more complex in the postprandial state, where gastric emptying,

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  • Tracee infusion that mimics glucose appearance in blood to maintain a constant tracer-to-tracee ratio ensures accurate measures of glucose fluxes.

    “Tracee infusion that mimics glucose appearance in blood to maintain a constant tracer-to-tracee ratio ensures accurate measures of glucose fluxes [24].”

    From Modeling the effect of glucagon on endogenous glucose production in healthy individuals under meal-like conditions (2025) · cites this paper as [24]

    The sections “Measurement of glucose turnover using a clamp” and “Glucose turnover in meal studies: triple-tracer methodology” (page numbers unavailable) say that maintaining a relatively constant tracer-to-tracee ratio enables accurate glucose-turnover measurements. But the infusion adjusted to mimic glucose appearance is a tracer infusion, not a tracee infusion as the claim states. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

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References 42 · Cited by 67

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Authors
Vella, A., & Rizza, R. A.
Affiliations
Mayo Clinic (US)
Corresponding author
Vella, Adrian
ORCID
Vella, Adrian
Venue
Diabetes
Publisher
American Diabetes Association
Published
2009-10-01
Volume
58
Issue
10
Pages
2168-2174
ISSN
  • 0012-1797 (ISSN-L)
  • 1939-327X
DOI
10.2337/db09-0318
PMC
PMC2750215
OpenAlex
W2102006343
Cited by
67
References
42
Citations by year
2025: 5 · 2024: 1 · 2023: 4 · 2022: 6 · 2021: 3 · 2020: 1 · 2019: 5 · 2018: 12 · 2017: 2 · 2016: 7 · 2015: 6 · 2014: 7 · 2013: 3 · 2012: 3
FWCI
1.21
Type
journal article
Language
en
Open access
Licence
Locations
  • Diabetes (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
  • Mayo Clinic (UL1 RR024150, RR024150)
  • Università degli Studi di Padova
  • National Institutes of Health (RR024150, UL1-RR-024150-01, RR024150-01)
  • National Institute of Diabetes and Digestive and Kidney Diseases (R01DK029953, DK 29953, R01DK082396, DK078646, R37DK029953, R01DK078646)
  • National Center for Research Resources (RR-024150, RR‐024150‐01, UL1RR024150)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Management and Research
  • Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
Keywords
isotope tracers, carbohydrate metabolism, tracer techniques, endogenous glucose production, glucose disappearance, postprandial blood glucose, fasting blood glucose, gastric emptying
MeSH terms
Blood Glucose, Carbohydrates, Dietary Carbohydrates, Glucose, Humans, Infusions, Intravenous, Isotope Labeling, Isotopes, Kinetics, Models, Biological, Neutrons, Radioisotopes, Scintillation Counting, Sensitivity and Specificity, Splanchnic Circulation

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