Reduced Lipid Oxidation in Skeletal Muscle From Type 2 Diabetic Subjects May Be of Genetic Origin
Open accessAbstract
Insulin resistance in skeletal muscle in vivo is associated with reduced lipid oxidation and lipid accumulation. It is still uncertain whether changes in lipid metabolism represent an adaptive compensation at the cellular level or a direct expression of a genetic trait. Studies of palmitate metabolism in human myotubes established from control and type 2 diabetic subjects may solve this problem, as genetic defects are preserved and expressed in vitro. In this study, total uptake of palmitic acid was similar in myotubes established from both control and type 2 diabetic subjects under basal conditions and acute insulin stimulation. Myotubes established from diabetic subjects expressed a primary reduced palmitic acid oxidation to carbon dioxide with a concomitantly increased esterification of palmitic acid into phospholipids compared with control myotubes under basal conditions. Triacylglycerol (TAG) content and the incorporation of palmitic acid into diacylglycerol (DAG) and TAG at basal conditions did not vary between the groups. Acute insulin treatment significantly increased palmitate uptake and incorporation of palmitic acid into DAG and TAG in myotubes established from both study groups, but no difference was found in myotubes established from control and diabetic subjects. These results indicate that the reduced lipid oxidation in diabetic skeletal muscle in vivo may be of genetic origin; it also appears that TAG metabolism is not primarily affected in diabetic muscles under basal physiological conditions.
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Intrinsic defects in fat oxidation are present in the skeletal muscle of insulin-resistant subjects.
“Together, these data suggest that intrinsic defects in fat oxidation are present in the skeletal muscle of insulin-resistant subjects.”
From Metabolic flexibility and insulin resistance (2008) · cites this paper as [16]
Fat oxidation in response to palmitate is lower in myotubes from insulin-resistant vs. insulin-sensitive individuals.
“Such muscle mitochondrial dysfunction was suggested to be an intrinsic characteristic of muscle cells, since fat oxidation in response to palmitate was lower in myotubes from insulin-resistant vs. insulin-sensitive individuals ([16](#r16), [61](#r61)).”
From Metabolic flexibility and insulin resistance (2008) · cites this paper as [16]
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References 40 · Cited by 176
- Authors
- Gaster, M., Rustan, A. C., Aas, V., & Beck-Nielsen, H.
- Affiliations
- Odense University Hospital (DK)
- University of Oslo (NO)
- Department of Endocrinology, Odense University Hospital, Odense, Denmark
- Venue
- Diabetes
- Publisher
- American Diabetes Association
- Published
- 2004-03-01
- Volume
- 53
- Issue
- 3
- Pages
- 542-548
- ISSN
- 0012-1797 (ISSN-L)
- 1939-327X
- OpenAlex
- W2158254429
- Cited by
- 176
- References
- 40
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 2 · 2023: 4 · 2022: 5 · 2021: 8 · 2020: 10 · 2019: 6 · 2018: 8 · 2017: 7 · 2016: 7 · 2015: 9 · 2014: 5 · 2013: 13 · 2012: 13
- FWCI
- 4.50
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Diabetes (publishedVersion)
- PubMed (publishedVersion)
- University of Southern Denmark Research Portal (University of Southern Denmark) (submittedVersion)
- Funders
- Sundhed og Sygdom, Det Frie Forskningsråd
- Odense Universitetshospital
- Norges Forskningsråd
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Keywords
- type 2 diabetes, skeletal muscle, lipid oxidation, insulin resistance, palmitate metabolism, myotubes, palmitate uptake, triglycerides, diacylglycerol, triglyceride metabolism, genetic susceptibility
- MeSH terms
- Blood Glucose, Cells, Cultured, Diabetes Mellitus, Type 2, Diglycerides, Glycated Hemoglobin, Humans, Insulin, Middle Aged, Oxidation-Reduction, Reference Values, Triglycerides, Muscle, Skeletal, Muscle Fibers, Skeletal, Lipid Metabolism
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