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Effect of Hematocrit Concentration on Blood Glucose Value Determined on Glucometer II
Abstract
Nineteen patient blood samples each with modified hematocrit concentrations of approximately 20, 30, 40, 50, and 60%, were assayed for their glucose concentration by the Glucometer II. Blood removal from the test strip was by the one- and two-blot techniques. The reference method was the Yellow Springs Instruments (YSI) blood glucose analyzer. Glucometer II results were falsely high for the single blot (13-59%, mean 33%) and double blot (12-41%, mean 19%) at 20% hematocrit and falsely low at 60% hematocrit for the single blot (22-44%, mean 37%) and the double blot (26-49%, mean 43%). At 40-50% hematocrit, Glucometer II and YSI results agreed only for the one-blot technique.
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“In strip-based glucose assays, high hematocrit values, due to increased blood viscosity, can reduce the diffusion of plasma and determine underestimated results, whereas low hematocrit levels, like in anemia, are associated with overestimated readings 101,102.”
From Glucose biosensors in clinical practice: principles, limits and perspectives of currently used devices (2022) · cites this paper as [101]
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Cited by 38
Metadata
- Authors
- Barreau, P. B., & Buttery, J. E.
- Affiliations
- Queen Elizabeth Hospital (AU)
- Venue
- Diabetes Care
- Publisher
- American Diabetes Association
- Published
- 1988-02-01
- Volume
- 11
- Issue
- 2
- Pages
- 116-118
- ISSN
- 0149-5992 (ISSN-L)
- 1935-5548
- OpenAlex
- W1974416649
- Cited by
- 38
- References
- 0
- Citations by year
- 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2015: 2 · 2014: 1 · 2013: 2 · 2012: 3
- FWCI
- 0.61
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Diabetes Care (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Health Professions › Medical Laboratory Technology › Clinical Laboratory Practices and Quality Control
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Management and Research
- Physical Sciences › Chemistry › Analytical Chemistry › Electrochemical sensors and biosensors
- Keywords
- hematocrit, blood glucose measurement, blood glucose meters, blood sampling technique, point-of-care glucose testing
- MeSH terms
- Blood Glucose, Colorimetry, Diabetes Mellitus, False Negative Reactions, False Positive Reactions, Hematocrit, Humans, Reagent Strips
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- CoChat. (2026). Citation check: Effect of Hematocrit Concentration on Blood Glucose Value Determined on Glucometer II. https://app.cochat.ai/papers/10.2337/diacare.11.2.116
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- Barreau, P. B., & Buttery, J. E. (1988). Effect of Hematocrit Concentration on Blood Glucose Value Determined on Glucometer II. Diabetes Care. https://doi.org/10.2337/diacare.11.2.116
