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Cited by 32Dermatology and Skin DiseasesAntimicrobial Resistance in StaphylococcusContact Dermatitis and Allergies

An in vitro model for bacterial growth on human stratum corneum

Open access
Krieken, D, Ederveen, T, Hijum, S, Jansen, P, Melchers, W, Scheepers, P, Schalkwijk, J, Zeeuwen, P·2016·Acta Dermato Venereologica
DOI 10.2340/00015555-2401
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Abstract

The diversity and dynamics of the skin microbiome in health and disease have been studied recently, but adequate model systems to study skin microbiotas in vitro are largely lacking. We developed an in vitro system that mimics human stratum corneum, using human callus as substrate and nutrient source for bacterial growth. The growth of several commensal and pathogenic bacterial strains was measured for up to one week by counting colony-forming units or by quantitative PCR with strain-specific primers. Human skin pathogens were found to survive amidst a minimal microbiome consisting of 2 major skin commensals: Staphylococcus epidermidis and Propionibacterium acnes. In addition, complete microbiomes, taken from the backs of healthy volunteers, were inoculated and maintained using this system. This model may enable the modulation of skin microbiomes in vitro and allow testing of pathogens, biological agents and antibiotics in a medium-throughput format.

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  • van Der Krieken et al. developed a simplified human skin model consisting of the stratum corneum only (human callus on top of agar in phosphate-buffered saline) and planted the complete microbiota from the back of healthy volunteers onto it; the model supported growth of the most relevant strains and the complete set of skin microbes normally found in vivo, which remained relatively stable for 7天.

    “An interesting study was performed by van Der Krieken et al. [140], who planted the complete microbiota taken from the back of healthy volunteers onto an originally developed simplified human skin model, which consisted in the reproduction of the stratum corneum only by using human callus on top of agar in phosphate-buffered saline. Since in vivo skin bacteria live attached to the dead corneocytes”

    From Recreating Human Skin In Vitro: Should the Microbiota Be Taken into Account? (2024) · cites this paper as [140]

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Authors
Krieken, D., Ederveen, T., Hijum, S., Jansen, P., Melchers, W., Scheepers, P., Schalkwijk, J., & Zeeuwen, P.
Affiliations
  • Radboud University Medical Center (NL)
  • Radboud University Nijmegen (NL)
ORCID
Venue
Acta Dermato Venereologica
Publisher
MJS Publishing, Medical Journals Sweden AB
Published
2016
Volume
96
Issue
7
Pages
873-879
ISSN
0001-5555 (ISSN-L)
DOI
10.2340/00015555-2401
OpenAlex
W2441681023
Cited by
32
References
0
Citations by year
2026: 4 · 2025: 3 · 2024: 3 · 2023: 12 · 2022: 2 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 2
FWCI
1.20
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • Acta Dermato Venereologica (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • Socio-Environmental Systems Modeling (acceptedVersion, cc-by)
  • Radboud Repository (Radboud University) (submittedVersion)
  • Radboud Repository (Radboud University) (submittedVersion, other-oa)
  • Data Archiving and Networked Services (DANS) (submittedVersion, cc-by)
Funders
Nederlandse Brandwonden Stichting
Topics
  • Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
  • Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
  • Health Sciences › Medicine › Dermatology › Contact Dermatitis and Allergies
Keywords
skin microbiome, stratum corneum, in vitro skin model, bacterial growth, Staphylococcus epidermidis, Propionibacterium acnes, skin commensals, skin pathogens, bacterial survival, antibiotic testing, medium-throughput screening
MeSH terms
Bony Callus, Humans, Microbial Sensitivity Tests, Propionibacterium acnes, Pseudomonas aeruginosa, Skin, Staphylococcus aureus, Staphylococcus epidermidis, Stem Cells, Streptococcus pyogenes, Tetracycline, Polymerase Chain Reaction, Microbiota, In Vitro Techniques

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