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Immunohistochemical diagnosis of Neospora caninum in tissue sections
Abstract
SUMMARY: An avidin-biotin-peroxidase complex immunoperoxidase staining method was developed to detect Neospora caninum in formalin-fixed, paraffin-embedded tissue sections. Specific antiserum to N caninum was made in rabbits and used to probe tissues from dogs naturally and experimentally infected with N caninum. The test detected tachyzoites and bradyzoites of N caninum. A reaction was not observed to Toxoplasma gondii, Hammondia, hammondi, Sarcocystis cruzi, S capricanis, S tenella, Besnoitia jellisoni, Caryospora bigenetica, Hepatazoon canis, Atoxoplasma sp, or the organism causing canine dermal coccidiosis. When antiserum made in rabbits to T gondii was used in the test, reaction to N caninum was not observed.
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Cited by
Recent advances in Neospora and neosporosis
Dubey, J. P. (1999) · Veterinary Parasitology · doi:10.1016/s0304-4017(99)00044-8 · Cited by 414
Self-citationRedescription of Neospora caninum and its differentiation from related coccidia
Dubey, J. P., Barr, B. C., Barta, J. R., Bjerkås, I., Björkman, C., Blagburn, B. L., Bowman, D. D., Buxton, D., Ellis, J. T., Gottstein, B., et al. (2002) · International Journal for Parasitology · doi:10.1016/s0020-7519(02)00094-2 · Cited by 270
Self-citationCanine neosporosis: perspectives on pathogenesis and management
Silva, R., & Machado, G. (2016) · Veterinary Medicine: Research and Reports · doi:10.2147/vmrr.s76969 · Cited by 62
“Direct methods consist in the visualization of oocysts, tissue cysts, or tachyzoites, stained or not, by light microscopy, histopathology, immunohistochemistry, in vitro cell culture and in vivo isolation by gerbil and/or mouse bio assay, fecal flotation of dog feces, and molecular methods, ie, polymerase chain reacti…”
Metadata
- Authors
- Lindsay, D. S., & Dubey, J. P.
- Venue
- American Journal of Veterinary Research
- Published
- 1989-11-01
- Volume
- 50
- Issue
- 11
- Pages
- 1981-1983
- ISSN
- 0002-9645 (ISSN-L)
- OpenAlex
- W1577827349
- Cited by
- 376
- References
- 0
- FWCI
- 19.03
- Type
- journal article
- Language
- en
- Open access
- closed
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- Lindsay, D. S., & Dubey, J. P. (1989). Immunohistochemical diagnosis of Neospora caninum in tissue sections. American Journal of Veterinary Research. https://doi.org/10.2460/ajvr.1989.50.11.1981
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