The DOI matches its Crossref record.
The collagen scaffold supports hiPSC-derived NSC growth and restricts hiPSC
Abstract
The human induced pluripotent stem cells (hiPSC) are one of the promising candidates as patient specific cell source for autologous transplantation or modeling of diseases. The collagen (Col) scaffolds have been shown suitable to create in vitro biomimetic microenvironment for human neural stem cells, but their ability to accommodate stem cells at different stages of neural differentiation has not been verified yet. In this paper we compare lineage related hiPSC during neural differentiation for their ability to colonize Col scaffold. We have also focused on modification of collagen physicochemical properties with improved mechanical and thermal stability, without loss of its biological activity. The hiPSC expressing markers of pluripotency (OCT4, SOX2, NANOG) after neural commitment are NESTIN, GFAP, PDGFR alpha, beta-TUBULIN III, MAP-2, DCX, GalC positive. We have shown, that Col scaffold was not preferable for hiPSC culture, while the neurally committed population after seeding on Col scaffolds revealed good adhesion, viability, proliferation, along with sustaining markers of neuronal and glial differentiation. The Col scaffold-based 3D culture of hiPSC-NSCs may serve as a research tool for further translational studies.
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“This shift demonstrates that UV-induced covalent crosslinking dominates the high-temperature thermal response, stabilizing the material at the macroscopic level despite partial loss of native collagen organization [44].”
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Ylä‐Outinen, L., Harju, V., Joki, T., et al. (2019) · Macromolecular Bioscience · doi:10.1002/mabi.201900096 · Cited by 20
Bayram, K. K., Fitremann, J., Bayram, A., Yilmaz, Z., Mehmetbeyoğlu, E., Özkul, Y., & Rassoulzadegan, M. (2021) · Processes · doi:10.3390/pr9040716 · Cited by 8
“Highlighting the impact of the scaffold on the gene expression of the cultivated cells is thus very important in order to better select the right scaffold for a given application [9,10].”
BackgroundDąbrowska-Gralak, M., Sadło, J., Głuszewski, W., Łyczko, K., Przybytniak, G. K., & Lewandowska, H. (2022) · Materials Today Communications · doi:10.1016/j.mtcomm.2022.103255 · Cited by 11
Zaszczyńska, A., Zychowicz, M., Kołbuk, D., et al. (2025) · Molecules · doi:10.3390/molecules30051041 · Cited by 8
“Neural stem cells were derived from a human induced pluripotent stem cell (hiPSC) line following the protocol described by Buzanska et al. [93], with some modifications.”
Impact of Collagen on the Rheological and Transport Properties of Agarose Hydrogels
Richterová, V., Gjevik, A., Vaculík, O., Vejrosta, J., & Pekař, M. (2025) · Gels · doi:10.3390/gels11060396 · Cited by 6
Human Neural Stem Cell Expansion in Natural Polymer Scaffolds Under Chemically Defined Condition
Chang, F., James, M. M., Zhou, Y., Ando, Y., Zareie, H. M., Yang, J., & Zhang, M. (2024) · Advanced Biology · doi:10.1002/adbi.202400224 · Cited by 2
Whalen, M. S., Daso, R. E., Thomas, M. F., et al. (2020) · Journal of Sol-Gel Science and Technology · doi:10.1007/s10971-020-05434-5 · Cited by 2
Augustyniak, J., Kozłowska, H., & Bużańska, L. (2023) · Cells · doi:10.3390/cells12030390 · Cited by 1
“Analysis of EB Morphology Changes under MeHgCl Treatment Newly formed EBs were induced for spontaneous differentiation by Essential E6 Medium (Thermo Fisher Scientific, Waltham, MA, USA) for 14 days in 21% O2 [17] and simultaneously treated with MeHgCl (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) at different conce…”
MethodInfluentialSelf-citationAntunes, B. S. L., Pedrini, F., Komatsu, D., Asami, J., Dicko, C., & Duek, E. A. R. (2026) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2026.154129
Properties study of fish collagen and gelatin blend for high-temperature food applications
Chay, J. C. (2022) · DR-NTU (Nanyang Technological University) · doi:10.32657/10356/163018
Metadata
- Authors
- Buzanska, L.
- Venue
- Frontiers in Bioscience
- Publisher
- IMR Press
- Published
- 2019
- Volume
- 11
- Issue
- 1
- Pages
- 105-121
- ISSN
- 1944-7906 (ISSN-L)
- 1945-0516
- 1945-0524
- DOI
- 10.2741/s529
- OpenAlex
- W2922371500
- Cited by
- 10
- References
- 0
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 1
- FWCI
- 0.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Frontiers in Bioscience-Scholar (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- collagen scaffolds, human induced pluripotent stem cells, neural stem cells, neuronal differentiation, 3D cell culture, scaffold modification, mechanical and thermal stability, glial differentiation, cell adhesion and proliferation, stem cell markers, autologous transplantation
- MeSH terms
- Animals, Biocompatible Materials, Calorimetry, Differential Scanning, Cell Differentiation, Collagen, Humans, Neurons, Swine, Tendons, Porosity, Spectroscopy, Fourier Transform Infrared, Microscopy, Confocal, Coculture Techniques, Tissue Scaffolds, Induced Pluripotent Stem Cells, Neural Stem Cells
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