Optimization of Peptone Production from Soybeans
Open accessAbstract
Soybeans are the most popular source of vegetable protein for the people of Indonesia, having protein content ranging from 35%-38%.In the protein analysis of 100 mesh size soybean powder using the Kjeldahl method with the Kjeltec 2100 Distilling Unit, the protein content is 35.95.The high protein content of soybeans can be used for the manufacture of Peptone by hydrolysis process using the papain enzyme.This study used a ratio of 1:2 substrate concentration, 0.3% enzyme concentration, pH 5-7, and a temperature of 40 °C-60 °C.The results of this study obtained the highest total dissolved nitrogen value of 14.7264%, at a temperature of 50 °C, pH of solution 5 and hydrolysis time of 7 h.The total dissolved nitrogen value was compared with the total nitrogen value of the material as a value indicating the result of enzymatic hydrolysis.Peptone-hydrolyzed soybeans are used as an alternative medium for the growth of Escherichia coli ATCC 25922 and Candida albicans ATCC 1023.
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Cost issues associated with soya peptone have been addressed in prior research, and cost-effective methods of soya peptone synthesis using enzymatic hydrolysis of soya beans have been proposed.
“However, cost issues associated with this nitrogen source have been addressed in some prior research, and cost-effective methods of soya peptone synthesis using enzymatic hydrolysis of soya beans have been proposed [55,56].”
From Optimization of Bacillus subtilis PW12 biomass production using RSM: a preliminary study towards single-cell protein production for aquaculture (2024) · cites this paper as [56]
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Gracious, M., & Nandakumar, S. (2024) · Journal of Applied Biology & Biotechnology · doi:10.7324/jabb.2024.177031 · Cited by 2 · see citing works
“However, cost issues associated with this nitrogen source have been addressed in some prior research, and cost-effective methods of soya peptone synthesis using enzymatic hydrolysis of soya beans have been proposed [55,56].”
- Authors
- Rochmaningsih, Harismah, K., & Mulyaningtyas, A.
- Affiliations
- Muhammadiyah University of Surakarta (ID)
- Corresponding author
- Rochmaningsih
- ORCID
- Harismah, Kun
- Venue
- Atlantis Highlights in Chemistry and Pharmaceutical Sciences
- Publisher
- Atlantis Press
- Published
- 2022
- ISSN
- 2590-3195 (ISSN-L)
- OpenAlex
- W4318475406
- Cited by
- 0
- References
- 0
- FWCI
- 0.00
- Type
- conference paper
- Language
- en
- Open access
- hybrid
- dx.doi.org
- Licence
- cc-by-nc
- Locations
- Atlantis Highlights in Chemistry and Pharmaceutical Sciences/Atlantis highlights in chemistry and pharmaceutical sciences (publishedVersion, cc-by-nc)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Keywords
- soybean, enzymatic hydrolysis, papain, proteolysis, total dissolved nitrogen, hydrolysis temperature, hydrolysis pH, hydrolysis time, plant proteins
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